Restriction map for
pF1KB5024
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BciVI BfrI BglII BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HindIII
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AvaI 343 CTCGGG C^[CT]CG[GA]G
BcgI 1200 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1256 TGATCA T^GATCA
BlpI 1012 GCTGAGC GC^T[ACGT]AGC
BpmI 319 CTGGAG CTGGAG(16/14)
Bpu1102I 1012 GCTGAGC GC^T[ACGT]AGC
BsaAI 79 TACGTG [CT]AC^GT[GA]
BseRI 682 GAGGAG GAGGAG(10/8)
BsiEI 182 CGGCCG CG[GA][CT]^CG
BsiWI 829 CGTACG C^GTACG
BsmBI 178 GAGACG CGTCTC(1/5)
BsoBI 343 CTCGGG C^[CT]CG[GA]G
BspHI 230 TCATGA T^CATGA
BsrBI 1015 GAGCGG CCGCTC(-3/-3)
BsrFI 860 GCCGGC [GA]^CCGG[CT]
CelII 1012 GCTGAGC GC^T[ACGT]AGC
Cfr10I 860 GCCGGC [GA]^CCGG[CT]
DraIII 13 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 182 CGGCCG C^GGCCG
EarI 763 GAAGAG CTCTTC(1/4)
EclXI 182 CGGCCG C^GGCCG
Eco52I 182 CGGCCG C^GGCCG
FbaI 1256 TGATCA T^GATCA
HaeII 33 GGCGCT [GA]GCGC^[CT]
HincII 688 GTTAAC GT[CT]^[GA]AC
HindII 688 GTTAAC GT[CT]^[GA]AC
HinfI 640 GACTC G^A[ACGT]TC
HpaI 688 GTTAAC GTT^AAC
Ksp632I 763 GAAGAG CTCTTC(1/4)
MfeI 6 CAATTG C^AATTG
MunI 6 CAATTG C^AATTG
NaeI 860 GCCGGC GCC^GGC
NgoMIV 860 GCCGGC G^CCGGC
NspI 1218 GCATGC [GA]CATG^[CT]
PflMI 12 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 640 GACTC GAGTC(4/5)
PshAI 207 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 701 CAGCTG CAG^CTG
RcaI 230 TCATGA T^CATGA
SfiI 708 GGCCCAGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1027 CTCAAG C^T[CT][GA]AG
SphI 1218 GCATGC GCATG^C
Tsp509I 7 AATT ^AATT
Van91I 12 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 78 GTAC GT^AC
830 GTAC
AflIII 80 ACGTGT A^C[GA][CT]GT
467 ACGTGT
ApaI 22 GGGCCC GGGCC^C
734 GGGCCC
AspHI 492 GAGCTC G[AT]GC[AT]^C
841 GAGCTC
BbvCI 447 CCTCAGC CCTCAGC(-5/-2)
703 GCTGAGG
BcnI 378 CCGGG CC^[GC]GG
576 CCGGG
BfaI 139 CTAG C^TAG
1081 CTAG
BsiHKAI 492 GAGCTC G[AT]GC[AT]^C
841 GAGCTC
BsmAI 178 GAGAC GTCTC(1/5)
1157 GAGAC
BspMI 921 GCAGGT ACCTGC(4/8)
1278 GCAGGT
EcoT14I 1073 CCTTGG C^C[AT][AT]GG
1127 CCAAGG
FauI 161 CCCGC CCCGC(4/6)
402 GCGGG
HgaI 507 GACGC GACGC(5/10)
565 GACGC
MaeI 139 CTAG C^TAG
1081 CTAG
MaeIII 205 GTGAC ^GT[ACGT]AC
1171 GTCAC
MseI 199 TTAA T^TAA
689 TTAA
MspA1I 162 CCGCTG C[AC]G^C[GT]G
701 CAGCTG
NciI 378 CCGGG CC^[GC]GG
576 CCGGG
PpuMI 346 GGGACCC [GA]G^G[AT]CC[CT]
923 AGGTCCT
PstI 310 CTGCAG CTGCA^G
727 CTGCAG
RsaI 78 GTAC GT^AC
830 GTAC
SacI 492 GAGCTC GAGCT^C
841 GAGCTC
StyI 1073 CCTTGG C^C[AT][AT]GG
1127 CCAAGG
Tru9I 199 TTAA T^TAA
689 TTAA
Tsp45I 205 GTGAC ^GT[GC]AC
1171 GTCAC
XcmI 26 CCAACGAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1064 CCAAGATGGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BglI BmyI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI TspRI TthHB8I
XhoII