Restriction map for
pF1KE0693
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# NO CUT ENZYMES ( 166 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsmI Bsp1286I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MluNI MroI MscI MseI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tsp45I
TspRI Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 695 GACGTC GACGT^C
AcsI 566 GAATTC [GA]^AATT[CT]
AcyI 695 GACGTC G[GA]^CG[CT]C
AfaI 384 GTAC GT^AC
AluI 551 AGCT AG^CT
ApoI 566 GAATTC [GA]^AATT[CT]
Asp700I 290 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 476 CTCGAG C^[CT]CG[GA]G
AvaII 427 GGACC G^G[AT]CC
BbvI 329 GCAGC GCAGC(8/12)
BclI 456 TGATCA T^GATCA
BglII 817 AGATCT A^GATCT
BsaHI 695 GACGTC G[GA]^CG[CT]C
BsaJI 360 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 311 ACCGGA [AT]^CCGG[AT]
BseRI 659 GAGGAG GAGGAG(10/8)
BsmBI 237 GAGACG CGTCTC(1/5)
BsoBI 476 CTCGAG C^[CT]CG[GA]G
Bsp1407I 383 TGTACA T^GTACA
BspMI 95 GCAGGT ACCTGC(4/8)
BsrFI 272 ACCGGC [GA]^CCGG[CT]
BsrGI 383 TGTACA T^GTACA
BssSI 77 CTCGTG CACGAG(-5/-1)
BstF5I 555 GGATG GGATG(2/0)
CfoI 598 GCGC GCG^C
Cfr10I 272 ACCGGC [GA]^CCGG[CT]
Cfr13I 427 GGACC G^G[ACGT]CC
EcoRI 566 GAATTC G^AATTC
EcoT14I 360 CCTTGG C^C[AT][AT]GG
FbaI 456 TGATCA T^GATCA
FokI 555 GGATG GGATG(9/13)
HaeIII 373 GGCC GG^CC
HgaI 839 GACGC GACGC(5/10)
HhaI 598 GCGC GCG^C
Hin1I 695 GACGTC G[GA]^CG[CT]C
HinP1I 598 GCGC G^CGC
HphI 778 TCACC GGTGA(8/7)
MaeIII 200 GTTAC ^GT[ACGT]AC
NlaIII 624 CATG CATG^
NlaIV 284 GGAGCC GG[ACGT]^[ACGT]CC
PaeR7I 476 CTCGAG C^TCGAG
PflMI 430 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 384 GTAC GT^AC
Sau96I 427 GGACC G^G[ACGT]CC
SfaNI 617 GCATC GCATC(5/9)
SfcI 828 CTACAG C^T[GA][CT]AG
SmlI 476 CTCGAG C^T[CT][GA]AG
SspBI 383 TGTACA T^GTACA
StyI 360 CCTTGG C^C[AT][AT]GG
TseI 329 GCAGC G^C[AT]GC
Tsp509I 567 AATT ^AATT
Van91I 430 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 476 CTCGAG C^TCGAG
XmnI 290 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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BsrI 325 ACTGG ACTGG(1/-1)
466 ACTGG
BssKI 167 CCAGG ^CC[ACGT]GG
613 CCAGG
BstNI 167 CCAGG CC^[AT]GG
613 CCAGG
DdeI 47 CTCAG C^T[ACGT]AG
376 CTCAG
EcoRII 167 CCAGG ^CC[AT]GG
613 CCAGG
HapII 273 CCGG C^CGG
312 CCGG
HpaII 273 CCGG C^CGG
312 CCGG
MaeII 198 ACGT A^CGT
696 ACGT
MslI 618 CATCTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
640 CATAGTGGTG
MspI 273 CCGG C^CGG
312 CCGG
MvaI 167 CCAGG CC^[AT]GG
613 CCAGG
MwoI 462 GCGAACTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
833 GCCCAAGACGC
PleI 514 GACTC GAGTC(4/5)
37 GAGTC
ScrFI 167 CCAGG CC^[ACGT]GG
613 CCAGG
TfiI 139 GAATC G^A[AT]TC
703 GATTC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AlwI BbsI BpuAI BsaBI
BsiYI BslI BsmAI BstYI DpnII
Fnu4HI HinfI ItaI MamI MboI
MboII MflI MnlI NdeII Sau3AI
TaqI TthHB8I XhoII