Restriction map for
pF1KB0401
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MfeI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TspRI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 453 GAATTT [GA]^AATT[CT]
AfaI 423 GTAC GT^AC
AlwNI 287 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 453 GAATTT [GA]^AATT[CT]
BanI 343 GGCACC G^G[CT][GA]CC
BbvCI 40 CCTCAGC CCTCAGC(-5/-2)
BbvI 372 GCTGC GCAGC(8/12)
BclI 120 TGATCA T^GATCA
BfaI 205 CTAG C^TAG
BpmI 227 CTCCAG CTGGAG(16/14)
BsaBI 196 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 383 CCATGG C^C[ACGT][ACGT]GG
Bsp1407I 422 TGTACA T^GTACA
BsrGI 422 TGTACA T^GTACA
BssKI 229 CCAGG ^CC[ACGT]GG
BstAPI 159 GCATGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 229 CCAGG CC^[AT]GG
BstYI 288 AGATCC [GA]^GATC[CT]
CfoI 36 GCGC GCG^C
Cfr13I 232 GGCCC G^G[ACGT]CC
DdeI 41 CTCAG C^T[ACGT]AG
DraII 231 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 383 CCATGG C^C[GA][CT]GG
EcoO109I 231 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 229 CCAGG ^CC[AT]GG
EcoT14I 383 CCATGG C^C[AT][AT]GG
FbaI 120 TGATCA T^GATCA
Fnu4HI 372 GCTGC GC^[ACGT]GC
HaeII 35 AGCGCC [GA]GCGC^[CT]
HhaI 36 GCGC GCG^C
HinP1I 36 GCGC G^CGC
ItaI 372 GCTGC GC^[ACGT]GC
MaeI 205 CTAG C^TAG
MaeIII 81 GTCAC ^GT[ACGT]AC
MamI 196 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 288 AGATCC [GA]^GATC[CT]
MseI 277 TTAA T^TAA
MvaI 229 CCAGG CC^[AT]GG
NcoI 383 CCATGG C^CATGG
RsaI 423 GTAC GT^AC
Sau96I 232 GGCCC G^G[ACGT]CC
ScrFI 229 CCAGG CC^[ACGT]GG
SmlI 363 CTTGAG C^T[CT][GA]AG
SspBI 422 TGTACA T^GTACA
StuI 104 AGGCCT AGG^CCT
StyI 383 CCATGG C^C[AT][AT]GG
Tru9I 277 TTAA T^TAA
TseI 372 GCTGC G^C[AT]GC
Tsp45I 81 GTCAC ^GT[GC]AC
XbaI 204 TCTAGA T^CTAGA
XhoII 288 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 8 enzymes) #
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AluI 371 AGCT AG^CT
419 AGCT
AlwI 289 GATCC GGATC(4/5)
390 GGATC
BsrI 340 ACTGG ACTGG(1/-1)
78 CCAGT
HaeIII 105 GGCC GG^CC
232 GGCC
HinfI 87 GATTC G^A[ACGT]TC
186 GAATC
MwoI 36 GCGCCCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
159 GCATGATATGC
SfaNI 208 GATGC GCATC(5/9)
479 GCATC
TfiI 87 GATTC G^A[AT]TC
186 GAATC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BstF5I Cac8I DpnII FokI HphI
MboI MboII MnlI NdeII NlaIII
NlaIV Sau3AI TaqI Tsp509I TthHB8I