Restriction map for
pF1KB3477
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
Acc65I AccI AccII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BseRI
BsgI BsiHKAI BsiWI BsmI Bsp1286I
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MluNI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuII RcaI
RsaI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 265 GACGTC GACGT^C
AccIII 140 TCCGGA T^CCGGA
AcsI 409 AAATTC [GA]^AATT[CT]
AcyI 265 GACGTC G[GA]^CG[CT]C
AlwI 138 GATCC GGATC(4/5)
Aor51HI 203 AGCGCT AGC^GCT
ApoI 409 AAATTC [GA]^AATT[CT]
AvaI 5 CCCGGG C^[CT]CG[GA]G
BglII 179 AGATCT A^GATCT
BsaAI 225 CACGTA [CT]AC^GT[GA]
BsaBI 423 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 265 GACGTC G[GA]^CG[CT]C
BsaI 442 GAGACC GGTCTC(1/5)
BsaWI 140 TCCGGA [AT]^CCGG[AT]
BseAI 140 TCCGGA T^CCGGA
BsiEI 119 CGATCG CG[GA][CT]^CG
BsiYI 141 CCGGACCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 141 CCGGACCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 391 GAGACG CGTCTC(1/5)
BsoBI 5 CCCGGG C^[CT]CG[GA]G
BspEI 140 TCCGGA T^CCGGA
BsrBI 376 GAGCGG CCGCTC(-3/-3)
BstF5I 429 CATCC GGATG(2/0)
BstNI 525 CCTGG CC^[AT]GG
CpoI 142 CGGACCG CG^G[AT]CCG
DraII 238 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco47III 203 AGCGCT AGC^GCT
EcoO109I 238 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 525 CCTGG ^CC[AT]GG
EcoT14I 452 CCAAGG C^C[AT][AT]GG
FauI 193 GCGGG CCCGC(4/6)
FokI 429 CATCC GGATG(9/13)
HaeIII 3 GGCC GG^CC
Hin1I 265 GACGTC G[GA]^CG[CT]C
HinfI 280 GAGTC G^A[ACGT]TC
MaeIII 268 GTCAC ^GT[ACGT]AC
MamI 423 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 140 TCCGGA T^CCGGA
MvaI 525 CCTGG CC^[AT]GG
MwoI 50 GCGGTGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 280 GAGTC GAGTC(4/5)
PpuMI 238 GGGACCC [GA]G^G[AT]CC[CT]
PstI 175 CTGCAG CTGCA^G
PvuI 119 CGATCG CGAT^CG
RsrII 142 CGGACCG CG^G[AT]CCG
SfcI 175 CTGCAG C^T[GA][CT]AG
SmaI 5 CCCGGG CCC^GGG
SspI 345 AATATT AAT^ATT
StyI 452 CCAAGG C^C[AT][AT]GG
TaqI 440 TCGA T^CGA
Tsp45I 268 GTCAC ^GT[GC]AC
Tsp509I 410 AATT ^AATT
TspRI 273 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 440 TCGA T^CGA
XcmI 116 CCACGATCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BsmAI 391 GAGAC GTCTC(1/5)
442 GAGAC
BsrI 272 CCAGT ACTGG(1/-1)
432 CCAGT
BstYI 137 AGATCC [GA]^GATC[CT]
179 AGATCT
CfoI 204 GCGC GCG^C
449 GCGC
DdeI 99 CTCAG C^T[ACGT]AG
374 CTGAG
DsaI 146 CCGTGG C^C[GA][CT]GG
243 CCACGG
EarI 22 CTCTTC CTCTTC(1/4)
472 GAAGAG
HaeII 203 AGCGCT [GA]GCGC^[CT]
448 AGCGCC
HhaI 204 GCGC GCG^C
449 GCGC
HinP1I 204 GCGC G^CGC
449 GCGC
HincII 289 GTCAAC GT[CT]^[GA]AC
463 GTCAAC
HindII 289 GTCAAC GT[CT]^[GA]AC
463 GTCAAC
Ksp632I 22 CTCTTC CTCTTC(1/4)
472 GAAGAG
MflI 137 AGATCC [GA]^GATC[CT]
179 AGATCT
MspA1I 48 CAGCGG C[AC]G^C[GT]G
191 CAGCGG
NlaIV 238 GGGACC GG[ACGT]^[ACGT]CC
239 GGACCC
XhoII 137 AGATCC [GA]^GATC[CT]
179 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsmFI BssKI Cfr13I DpnII
Fnu4HI HapII HpaII HphI ItaI
MaeII MboI MboII MnlI MspI
NciI NdeII Sau3AI Sau96I ScrFI
SfaNI TseI