Restriction map for
pF1KE1090
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbvCI BcgI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmI BspDI BspHI BspLU11I BsrBI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccIII 1730 TCCGGA T^CCGGA
Asp700I 297 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 358 CCCGAG C^[CT]CG[GA]G
BanII 1591 GAGCCC G[GA]GC[CT]^C
BbrPI 100 CACGTG CAC^GTG
BciVI 363 GTATCC GTATCC(6/5)
BclI 104 TGATCA T^GATCA
BglII 764 AGATCT A^GATCT
BsaAI 100 CACGTG [CT]AC^GT[GA]
BseAI 1730 TCCGGA T^CCGGA
BsmBI 40 GAGACG CGTCTC(1/5)
BsoBI 358 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1004 TGTACA T^GTACA
BspEI 1730 TCCGGA T^CCGGA
BspMI 1293 ACCTGC ACCTGC(4/8)
BsrGI 1004 TGTACA T^GTACA
BssSI 612 CACGAG CACGAG(-5/-1)
EcoRI 437 GAATTC G^AATTC
EcoT14I 188 CCAAGG C^C[AT][AT]GG
FbaI 104 TGATCA T^GATCA
HgaI 378 GACGC GACGC(5/10)
HincII 1135 GTCAAC GT[CT]^[GA]AC
HindII 1135 GTCAAC GT[CT]^[GA]AC
MroI 1730 TCCGGA T^CCGGA
PmaCI 100 CACGTG CAC^GTG
PmlI 100 CACGTG CAC^GTG
ScaI 890 AGTACT AGT^ACT
SfaNI 1517 GCATC GCATC(5/9)
SspBI 1004 TGTACA T^GTACA
StyI 188 CCAAGG C^C[AT][AT]GG
XmnI 297 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 437 GAATTC [GA]^AATT[CT]
979 AAATTT
AflIII 851 ACGTGT A^C[GA][CT]GT
1560 ACACGT
Alw44I 181 GTGCAC G^TGCAC
250 GTGCAC
AlwNI 1077 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
1710 CAGCTGCTG
ApaLI 181 GTGCAC G^TGCAC
250 GTGCAC
ApoI 437 GAATTC [GA]^AATT[CT]
979 AAATTT
AvaII 420 GGACC G^G[AT]CC
1548 GGTCC
BbsI 477 GAAGAC GAAGAC(2/6)
934 GTCTTC
BpuAI 477 GAAGAC GAAGAC(2/6)
934 GTCTTC
BsrFI 3 GCCGGC [GA]^CCGG[CT]
1628 GCCGGC
CfoI 1603 GCGC GCG^C
1668 GCGC
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
1628 GCCGGC
DraII 524 AGGCCCC [GA]G^G[ACGT]CC[CT]
1510 GGGGCCT
EaeI 597 CGGCCA [CT]^GGCC[GA]
1037 TGGCCG
EarI 520 GAAGAG CTCTTC(1/4)
1746 GAAGAG
EcoO109I 524 AGGCCCC [GA]G^G[ACGT]CC[CT]
1510 GGGGCCT
FauI 371 CCCGC CCCGC(4/6)
423 CCCGC
HaeII 1602 GGCGCT [GA]GCGC^[CT]
1667 GGCGCT
HhaI 1603 GCGC GCG^C
1668 GCGC
HinP1I 1603 GCGC G^CGC
1668 GCGC
Ksp632I 520 GAAGAG CTCTTC(1/4)
1746 GAAGAG
MseI 86 TTAA T^TAA
1192 TTAA
NaeI 3 GCCGGC GCC^GGC
1628 GCCGGC
NgoMIV 3 GCCGGC G^CCGGC
1628 GCCGGC
PflMI 83 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1680 CCAGGACATGG
Tru9I 86 TTAA T^TAA
1192 TTAA
Tsp45I 52 GTCAC ^GT[GC]AC
140 GTGAC
Van91I 83 CCATTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1680 CCAGGACATGG
XcmI 506 CCATCGAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1319 CCAGGCTGCCCCTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BamHI BanI BbvI BcnI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XhoII