Restriction map for
pF1KE5606
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AvaI AvaII
AviII AvrII BamHI BbrPI BbvCI
BbvI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspEI BspHI
BspMI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HgaI HincII HindII HindIII HpaI
ItaI KpnI Ksp632I KspI MamI
MluI MroI MspA1I MvnI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspI StuI SwaI TseI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 1236 CCGC CCGC(-3/-1)
AcyI 1208 GGCGCC G[GA]^CG[CT]C
AflIII 647 ACATGT A^C[GA][CT]GT
AlwI 1498 GATCC GGATC(4/5)
Aor51HI 918 AGCGCT AGC^GCT
AspI 1186 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1208 GGCGCC G^G[CT][GA]CC
BanII 41 GGGCTC G[GA]GC[CT]^C
BbeI 1208 GGCGCC GGCGC^C
BcnI 798 CCGGG CC^[GC]GG
BglII 259 AGATCT A^GATCT
BmyI 41 GGGCTC G[AGT]GC[ACT]^C
BsaAI 565 TACGTG [CT]AC^GT[GA]
BsaHI 1208 GGCGCC G[GA]^CG[CT]C
BsaI 168 GGTCTC GGTCTC(1/5)
BseRI 968 GAGGAG GAGGAG(10/8)
BsgI 368 GTGCAG GTGCAG(16/14)
BsiYI 433 CCATATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 433 CCATATTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 41 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 1004 TGTACA T^GTACA
BspDI 1202 ATCGAT AT^CGAT
BspLU11I 647 ACATGT A^CATGT
BsrGI 1004 TGTACA T^GTACA
BssSI 1052 CACGAG CACGAG(-5/-1)
BstAPI 1510 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I 1120 GGGCC G^G[ACGT]CC
ClaI 1202 ATCGAT AT^CGAT
Eco47III 918 AGCGCT AGC^GCT
EcoRI 808 GAATTC G^AATTC
EcoRV 484 GATATC GAT^ATC
EcoT14I 214 CCTTGG C^C[AT][AT]GG
HapII 798 CCGG C^CGG
Hin1I 1208 GGCGCC G[GA]^CG[CT]C
HpaII 798 CCGG C^CGG
KasI 1208 GGCGCC G^GCGCC
MfeI 1425 CAATTG C^AATTG
MslI 346 CATATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 798 CCGG C^CGG
MunI 1425 CAATTG C^AATTG
NarI 1208 GGCGCC GG^CGCC
NciI 798 CCGGG CC^[GC]GG
NdeI 346 CATATG CA^TATG
NspI 647 ACATGT [GA]CATG^[CT]
PflFI 1186 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 433 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 713 CTGCAG CTGCA^G
Sau96I 1120 GGGCC G^G[ACGT]CC
SbfI 712 CCTGCAGG CCTGCA^GG
SfcI 713 CTGCAG C^T[GA][CT]AG
SfoI 1208 GGCGCC GGC^GCC
Sse8387I 712 CCTGCAGG CCTGCA^GG
SspBI 1004 TGTACA T^GTACA
StyI 214 CCTTGG C^C[AT][AT]GG
Tth111I 1186 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 433 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 381 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
699 CAGTAACTG
BalI 1075 TGGCCA TGG^CCA
1251 TGGCCA
BbsI 536 GTCTTC GAAGAC(2/6)
1373 GTCTTC
BfaI 296 CTAG C^TAG
998 CTAG
BpuAI 536 GTCTTC GAAGAC(2/6)
1373 GTCTTC
BsaJI 214 CCTTGG C^C[ACGT][ACGT]GG
405 CCCTGG
BsmAI 659 GAGAC GTCTC(1/5)
169 GTCTC
BsmFI 416 GGGAC GGGAC(10/14)
430 GTCCC
BsrDI 1181 GCAATG GCAATG(2/0)
1238 GCAATG
BstYI 259 AGATCT [GA]^GATC[CT]
1497 AGATCC
CfoI 919 GCGC GCG^C
1209 GCGC
HaeII 918 AGCGCT [GA]GCGC^[CT]
1208 GGCGCC
HhaI 919 GCGC GCG^C
1209 GCGC
HinP1I 919 GCGC G^CGC
1209 GCGC
HinfI 1319 GATTC G^A[ACGT]TC
1522 GAATC
MaeI 296 CTAG C^TAG
998 CTAG
MaeII 566 ACGT A^CGT
1448 ACGT
MflI 259 AGATCT [GA]^GATC[CT]
1497 AGATCC
MluNI 1075 TGGCCA TGG^CCA
1251 TGGCCA
MscI 1075 TGGCCA TGG^CCA
1251 TGGCCA
TaqI 488 TCGA T^CGA
1203 TCGA
TfiI 1319 GATTC G^A[AT]TC
1522 GAATC
TspRI 551 CACTG CA[GC]TG[ACGT][ACGT]^
777 CAGTG
TthHB8I 488 TCGA T^CGA
1203 TCGA
XcmI 433 CCATATTCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1290 CCAAGTGGCTTTTGG
XhoII 259 AGATCT [GA]^GATC[CT]
1497 AGATCC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BpmI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EaeI
EcoRII FokI HaeIII HphI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PshBI
RsaI Sau3AI ScrFI SfaNI Tru9I
Tsp45I Tsp509I