Restriction map for
pF1KB6947
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI CelII ClaI CpoI
DpnII DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MboI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NciI NdeI NdeII
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 529 GTATAC GT^[AC][GT]AC
AocI 220 CCTGAGG CC^T[ACGT]AGG
AvaII 104 GGACC G^G[AT]CC
BalI 66 TGGCCA TGG^CCA
BfaI 262 CTAG C^TAG
BseRI 79 GAGGAG GAGGAG(10/8)
BsmI 286 GCATTC GAATGC(1/-1)
BsrFI 178 ACCGGC [GA]^CCGG[CT]
Bst1107I 529 GTATAC GTA^TAC
BstZ17I 529 GTATAC GTA^TAC
Bsu36I 220 CCTGAGG CC^T[ACGT]AGG
CfoI 56 GCGC GCG^C
Cfr10I 178 ACCGGC [GA]^CCGG[CT]
Cfr13I 104 GGACC G^G[ACGT]CC
DdeI 221 CTGAG C^T[ACGT]AG
DsaI 44 CCATGG C^C[GA][CT]GG
EaeI 66 TGGCCA [CT]^GGCC[GA]
Eco81I 220 CCTGAGG CC^T[ACGT]AGG
EcoT14I 44 CCATGG C^C[AT][AT]GG
HapII 179 CCGG C^CGG
HhaI 56 GCGC GCG^C
HinP1I 56 GCGC G^CGC
HindIII 200 AAGCTT A^AGCTT
HinfI 571 GAGTC G^A[ACGT]TC
HpaII 179 CCGG C^CGG
MaeI 262 CTAG C^TAG
MluNI 66 TGGCCA TGG^CCA
MscI 66 TGGCCA TGG^CCA
MslI 230 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 232 CAGCTG C[AC]G^C[GT]G
MspI 179 CCGG C^CGG
NcoI 44 CCATGG C^CATGG
NheI 261 GCTAGC G^CTAGC
NlaIV 104 GGACCC GG[ACGT]^[ACGT]CC
PleI 571 GAGTC GAGTC(4/5)
PstI 36 CTGCAG CTGCA^G
PvuII 232 CAGCTG CAG^CTG
Sau96I 104 GGACC G^G[ACGT]CC
SfcI 36 CTGCAG C^T[GA][CT]AG
SspI 325 AATATT AAT^ATT
StyI 44 CCATGG C^C[AT][AT]GG
TspRI 63 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BbsI 123 GAAGAC GAAGAC(2/6)
349 GAAGAC
BpuAI 123 GAAGAC GAAGAC(2/6)
349 GAAGAC
BsaJI 44 CCATGG C^C[ACGT][ACGT]GG
163 CCTGGG
BssKI 163 CCTGG ^CC[ACGT]GG
585 CCAGG
BstNI 163 CCTGG CC^[AT]GG
585 CCAGG
DraI 111 TTTAAA TTT^AAA
300 TTTAAA
EcoRII 163 CCTGG ^CC[AT]GG
585 CCAGG
HaeIII 67 GGCC GG^CC
342 GGCC
HphI 370 TCACC GGTGA(8/7)
611 TCACC
MseI 112 TTAA T^TAA
301 TTAA
MvaI 163 CCTGG CC^[AT]GG
585 CCAGG
NlaIII 45 CATG CATG^
625 CATG
ScrFI 163 CCTGG CC^[ACGT]GG
585 CCAGG
Tru9I 112 TTAA T^TAA
301 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsiYI
BslI Cac8I EcoRI Fnu4HI ItaI
MboII MnlI MwoI TseI Tsp509I