Restriction map for
pF1KB5937
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BciVI BfaI
BfrI BlnI BsaAI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MluNI MscI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1063 CGCG CG^CG
AccIII 1070 TCCGGA T^CCGGA
AflIII 1352 ACGTGT A^C[GA][CT]GT
AlwNI 133 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 364 GGGCCC GGGCC^C
Asp700I 1026 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 871 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 115 GGCGCC G^G[CT][GA]CC
BbeI 115 GGCGCC GGCGC^C
BbvCI 474 GCTGAGG CCTCAGC(-5/-2)
BcgI 201 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1397 TGATCA T^GATCA
BglII 707 AGATCT A^GATCT
BseAI 1070 TCCGGA T^CCGGA
BsgI 406 GTGCAG GTGCAG(16/14)
BsiEI 1059 CGACCG CG[GA][CT]^CG
BsmI 248 GCATTC GAATGC(1/-1)
BspEI 1070 TCCGGA T^CCGGA
BspMI 1448 ACCTGC ACCTGC(4/8)
BsrBI 690 CCGCTC CCGCTC(-3/-3)
BsrDI 1362 CATTGC GCAATG(2/0)
BsrFI 611 ACCGGC [GA]^CCGG[CT]
BstBI 242 TTCGAA TT^CGAA
BstUI 1063 CGCG CG^CG
BstXI 1091 CCACGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 611 ACCGGC [GA]^CCGG[CT]
DraIII 1438 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 663 CGGCCA [CT]^GGCC[GA]
FbaI 1397 TGATCA T^GATCA
KasI 115 GGCGCC G^GCGCC
MaeII 1352 ACGT A^CGT
MroI 1070 TCCGGA T^CCGGA
MvnI 1063 CGCG CG^CG
NarI 115 GGCGCC GG^CGCC
NcoI 1334 CCATGG C^CATGG
NspV 242 TTCGAA TT^CGAA
PflFI 871 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 504 GACTC GAGTC(4/5)
PvuII 421 CAGCTG CAG^CTG
SacI 759 GAGCTC GAGCT^C
SapI 123 GAAGAGC GCTCTTC(1/4)
ScaI 1046 AGTACT AGT^ACT
SfoI 115 GGCGCC GGC^GCC
SfuI 242 TTCGAA TT^CGAA
SmlI 682 CTCAAG C^T[CT][GA]AG
StuI 1272 AGGCCT AGG^CCT
TfiI 336 GATTC G^A[AT]TC
Tth111I 871 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 293 CCAGTGGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1026 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 841 GATCC GGATC(4/5)
1134 GGATC
AspHI 97 GAGCAC G[AT]GC[AT]^C
759 GAGCTC
BbsI 510 GAAGAC GAAGAC(2/6)
1309 GTCTTC
BglI 70 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1332 GCCCATGGGGC
BlpI 82 GCTGAGC GC^T[ACGT]AGC
573 GCTGAGC
Bpu1102I 82 GCTGAGC GC^T[ACGT]AGC
573 GCTGAGC
BpuAI 510 GAAGAC GAAGAC(2/6)
1309 GTCTTC
BsaI 1078 GAGACC GGTCTC(1/5)
1141 GGTCTC
BsaWI 1038 TCCGGT [AT]^CCGG[AT]
1070 TCCGGA
BseRI 202 GAGGAG GAGGAG(10/8)
465 GAGGAG
BsiHKAI 97 GAGCAC G[AT]GC[AT]^C
759 GAGCTC
BstYI 707 AGATCT [GA]^GATC[CT]
840 AGATCC
CelII 82 GCTGAGC GC^T[ACGT]AGC
573 GCTGAGC
DsaI 642 CCACGG C^C[GA][CT]GG
1334 CCATGG
HinfI 336 GATTC G^A[ACGT]TC
504 GACTC
MaeIII 502 GTGAC ^GT[ACGT]AC
812 GTGAC
MflI 707 AGATCT [GA]^GATC[CT]
840 AGATCC
MslI 729 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1248 CATGGAGGTG
PstI 856 CTGCAG CTGCA^G
1450 CTGCAG
SfcI 856 CTGCAG C^T[GA][CT]AG
1450 CTGCAG
SmaI 212 CCCGGG CCC^GGG
368 CCCGGG
Tsp45I 502 GTGAC ^GT[GC]AC
812 GTGAC
XhoII 707 AGATCT [GA]^GATC[CT]
840 AGATCC
XmaCI 212 CCCGGG C^CCGGG
368 CCCGGG
XmaI 212 CCCGGG C^CCGGG
368 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I Van91I