Restriction map for
pF1KB8193
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII AccI AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
HphI KasI Ksp632I KspI MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluI MseI MslI
MunI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
Sau3AI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 162 GGTACC G^GTACC
AccIII 315 TCCGGA T^CCGGA
AfaI 163 GTAC GT^AC
AlwNI 260 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 90 GGGCCC GGGCC^C
Asp718I 162 GGTACC G^GTACC
AspHI 307 GAGCTC G[AT]GC[AT]^C
AvaII 268 GGACC G^G[AT]CC
BalI 65 TGGCCA TGG^CCA
BanI 162 GGTACC G^G[CT][GA]CC
BbvCI 100 GCTGAGG CCTCAGC(-5/-2)
BsaWI 315 TCCGGA [AT]^CCGG[AT]
BseAI 315 TCCGGA T^CCGGA
BseRI 103 GAGGAG GAGGAG(10/8)
BsiHKAI 307 GAGCTC G[AT]GC[AT]^C
BsmFI 172 GTCCC GGGAC(10/14)
BspEI 315 TCCGGA T^CCGGA
BsrI 159 ACTGG ACTGG(1/-1)
BstF5I 294 CATCC GGATG(2/0)
DraII 89 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 89 AGGGCCC [GA]G^G[ACGT]CC[CT]
FokI 294 CATCC GGATG(9/13)
HaeII 6 GGCGCT [GA]GCGC^[CT]
HgaI 215 GCGTC GACGC(5/10)
HinfI 170 GAGTC G^A[ACGT]TC
KpnI 162 GGTACC GGTAC^C
MluNI 65 TGGCCA TGG^CCA
MroI 315 TCCGGA T^CCGGA
MscI 65 TGGCCA TGG^CCA
NlaIII 129 CATG CATG^
PflMI 259 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 170 GAGTC GAGTC(4/5)
PstI 238 CTGCAG CTGCA^G
PvuII 98 CAGCTG CAG^CTG
RsaI 163 GTAC GT^AC
SacI 307 GAGCTC GAGCT^C
SbfI 237 CCTGCAGG CCTGCA^GG
SmaI 74 CCCGGG CCC^GGG
Sse8387I 237 CCTGCAGG CCTGCA^GG
TspRI 136 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 259 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 74 CCCGGG C^CCGGG
XmaI 74 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AccII 22 CGCG CG^CG
214 CGCG
AvaI 74 CCCGGG C^[CT]CG[GA]G
174 CCCGAG
BsoBI 74 CCCGGG C^[CT]CG[GA]G
174 CCCGAG
BspMI 236 ACCTGC ACCTGC(4/8)
240 GCAGGT
BstUI 22 CGCG CG^CG
214 CGCG
EaeI 65 TGGCCA [CT]^GGCC[GA]
251 TGGCCG
FauI 146 CCCGC CCCGC(4/6)
153 GCGGG
MspA1I 98 CAGCTG C[AC]G^C[GT]G
109 CCGCTG
MvnI 22 CGCG CG^CG
214 CGCG
SfcI 208 CTACAG C^T[GA][CT]AG
238 CTGCAG
TaqI 221 TCGA T^CGA
233 TCGA
TthHB8I 221 TCGA T^CGA
233 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BlpI BmyI Bpu1102I BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CelII CfoI Cfr13I DdeI EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspI MvaI
MwoI NciI NlaIV Sau96I ScrFI
TseI