Restriction map for
pF1KB6922
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
ClaI CpoI DraI DsaI Eam1105I
EarI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AfaI 274 GTAC GT^AC
AlwNI 413 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 229 CCTCAGG CC^T[ACGT]AGG
Aor51HI 647 AGCGCT AGC^GCT
ApaI 73 GGGCCC GGGCC^C
AvaII 485 GGACC G^G[AT]CC
BanI 672 GGTGCC G^G[CT][GA]CC
BbrPI 539 CACGTG CAC^GTG
BfaI 22 CTAG C^TAG
BsaAI 539 CACGTG [CT]AC^GT[GA]
BsgI 636 CTGCAC GTGCAG(16/14)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsrBI 571 GAGCGG CCGCTC(-3/-3)
Bsu36I 229 CCTCAGG CC^T[ACGT]AGG
DdeI 230 CTCAG C^T[ACGT]AG
DraIII 277 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 391 GACGAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco47III 647 AGCGCT AGC^GCT
Eco52I 8 CGGCCG C^GGCCG
Eco81I 229 CCTCAGG CC^T[ACGT]AGG
EcoNI 552 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI 22 CTAG C^TAG
MaeIII 542 GTGAC ^GT[ACGT]AC
MboII 653 TCTTC GAAGA(8/7)
MseI 446 TTAA T^TAA
MslI 504 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 590 CAGCTG C[AC]G^C[GT]G
NlaIII 480 CATG CATG^
PaeR7I 166 CTCGAG C^TCGAG
PmaCI 539 CACGTG CAC^GTG
PmlI 539 CACGTG CAC^GTG
PstI 628 CTGCAG CTGCA^G
PvuII 590 CAGCTG CAG^CTG
RsaI 274 GTAC GT^AC
SexAI 179 ACCAGGT A^CC[AT]GGT
SfcI 628 CTGCAG C^T[GA][CT]AG
SfiI 9 GGCCGGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tru9I 446 TTAA T^TAA
Tsp45I 542 GTGAC ^GT[GC]AC
XhoI 166 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 15 CGCG CG^CG
147 CGCG
AvaI 166 CTCGAG C^[CT]CG[GA]G
427 CCCGAG
BsaI 169 GAGACC GGTCTC(1/5)
475 GAGACC
BseRI 394 GAGGAG GAGGAG(10/8)
430 GAGGAG
BsmAI 169 GAGAC GTCTC(1/5)
475 GAGAC
BsmFI 302 GGGAC GGGAC(10/14)
255 GTCCC
BsoBI 166 CTCGAG C^[CT]CG[GA]G
427 CCCGAG
BspMI 551 ACCTGC ACCTGC(4/8)
630 GCAGGT
BstUI 15 CGCG CG^CG
147 CGCG
DpnII 186 GATC ^GATC
369 GATC
DraII 72 GGGGCCC [GA]G^G[ACGT]CC[CT]
73 GGGCCCC
EaeI 8 CGGCCG [CT]^GGCC[GA]
152 CGGCCA
EcoO109I 72 GGGGCCC [GA]G^G[ACGT]CC[CT]
73 GGGCCCC
HaeII 647 AGCGCT [GA]GCGC^[CT]
665 AGCGCC
HincII 577 GTCAAC GT[CT]^[GA]AC
595 GTCAAC
HindII 577 GTCAAC GT[CT]^[GA]AC
595 GTCAAC
HinfI 253 GAGTC G^A[ACGT]TC
466 GAGTC
HphI 94 GGTGA GGTGA(8/7)
183 GGTGA
MboI 186 GATC ^GATC
369 GATC
MvnI 15 CGCG CG^CG
147 CGCG
NdeII 186 GATC ^GATC
369 GATC
PleI 253 GAGTC GAGTC(4/5)
466 GAGTC
Sau3AI 186 GATC ^GATC
369 GATC
SmlI 166 CTCGAG C^T[CT][GA]AG
643 CTCAAG
TspRI 53 CAGTG CA[GC]TG[ACGT][ACGT]^
130 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MnlI MspI MvaI
MwoI NaeI NciI NgoMIV NlaIV
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I