Restriction map for
pF1KB7863
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BanI BanII BbeI
BbvCI BcgI BclI BcnI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MflI MluI
MroI MspI MvnI MwoI NaeI
NarI NciI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 637 GTATAC GT^[AC][GT]AC
AciI 1610 GCGG CCGC(-3/-1)
AflII 481 CTTAAG C^TTAAG
AflIII 1540 ACGTGT A^C[GA][CT]GT
AlwNI 5 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 700 ATTAAT AT^TAAT
AsnI 700 ATTAAT AT^TAAT
AvaI 1044 CTCGGG C^[CT]CG[GA]G
AvaII 42 GGACC G^G[AT]CC
BalI 233 TGGCCA TGG^CCA
BfaI 650 CTAG C^TAG
BfrI 481 CTTAAG C^TTAAG
BmyI 1328 GTGCCC G[AGT]GC[ACT]^C
BsaBI 106 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 1079 GAGAC GTCTC(1/5)
BsmI 1513 GAATGC GAATGC(1/-1)
BsoBI 1044 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1328 GTGCCC G[AGT]GC[ACT]^C
Bst1107I 637 GTATAC GTA^TAC
BstBI 1640 TTCGAA TT^CGAA
BstZ17I 637 GTATAC GTA^TAC
DraII 41 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 21 CCATGG C^C[GA][CT]GG
EaeI 233 TGGCCA [CT]^GGCC[GA]
EcoO109I 41 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1559 GAATTC G^AATTC
EcoT22I 1057 ATGCAT ATGCA^T
FauI 1610 GCGGG CCCGC(4/6)
HgaI 486 GACGC GACGC(5/10)
HindIII 1616 AAGCTT A^AGCTT
MaeI 650 CTAG C^TAG
MamI 106 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 403 CAATTG C^AATTG
MluNI 233 TGGCCA TGG^CCA
MscI 233 TGGCCA TGG^CCA
MspA1I 5 CAGCTG C[AC]G^C[GT]G
MunI 403 CAATTG C^AATTG
NcoI 21 CCATGG C^CATGG
NsiI 1057 ATGCAT ATGCA^T
NspV 1640 TTCGAA TT^CGAA
PpuMI 41 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 700 ATTAAT AT^TAAT
PvuII 5 CAGCTG CAG^CTG
ScaI 1040 AGTACT AGT^ACT
SfaNI 210 GATGC GCATC(5/9)
SfuI 1640 TTCGAA TT^CGAA
SmlI 481 CTTAAG C^T[CT][GA]AG
SspI 631 AATATT AAT^ATT
TaqI 1641 TCGA T^CGA
TthHB8I 1641 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
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AluI 6 AGCT AG^CT
1617 AGCT
BbrPI 490 CACGTG CAC^GTG
1539 CACGTG
BbsI 1643 GAAGAC GAAGAC(2/6)
460 GTCTTC
BbvI 4 GCAGC GCAGC(8/12)
7 GCTGC
BciVI 398 GGATAC GTATCC(6/5)
1290 GTATCC
BpuAI 1643 GAAGAC GAAGAC(2/6)
460 GTCTTC
BsaAI 490 CACGTG [CT]AC^GT[GA]
1539 CACGTG
BseRI 160 GAGGAG GAGGAG(10/8)
311 GAGGAG
BsmFI 41 GGGAC GGGAC(10/14)
18 GTCCC
BsrDI 447 GCAATG GCAATG(2/0)
1605 GCAATG
DpnII 252 GATC ^GATC
1067 GATC
EcoT14I 21 CCATGG C^C[AT][AT]GG
321 CCAAGG
Fnu4HI 4 GCAGC GC^[ACGT]GC
7 GCTGC
HphI 577 GGTGA GGTGA(8/7)
152 TCACC
ItaI 4 GCAGC GC^[ACGT]GC
7 GCTGC
MboI 252 GATC ^GATC
1067 GATC
MslI 668 CATTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
920 CATATGAATG
NdeI 724 CATATG CA^TATG
920 CATATG
NdeII 252 GATC ^GATC
1067 GATC
NlaIV 41 GGGACC GG[ACGT]^[ACGT]CC
42 GGACCC
PmaCI 490 CACGTG CAC^GTG
1539 CACGTG
PmlI 490 CACGTG CAC^GTG
1539 CACGTG
Sau3AI 252 GATC ^GATC
1067 GATC
SfcI 198 CTACAG C^T[GA][CT]AG
864 CTATAG
StuI 1253 AGGCCT AGG^CCT
1448 AGGCCT
StyI 21 CCATGG C^C[AT][AT]GG
321 CCAAGG
TfiI 118 GATTC G^A[AT]TC
1403 GAATC
TseI 4 GCAGC G^C[AT]GC
7 GCTGC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI ApoI Asp700I BpmI
BsaJI BsiYI BslI BspHI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
EarI EcoRII FokI HaeIII HinfI
Ksp632I MaeII MaeIII MboII MnlI
MseI MvaI NlaIII NspI PleI
RcaI RsaI Sau96I ScrFI Tru9I
Tsp45I Tsp509I TspRI XmnI