Restriction map for
pF1KB6557
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 58 GTCTAC GT^[AC][GT]AC
AhdI 700 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 613 GATCC GGATC(4/5)
AspEI 700 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 71 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 114 GGGCTC G[GA]GC[CT]^C
BglI 245 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 998 AGATCT A^GATCT
BlpI 339 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 339 GCTCAGC GC^T[ACGT]AGC
BsgI 97 GTGCAG GTGCAG(16/14)
BsrBI 348 CCGCTC CCGCTC(-3/-3)
BsrDI 551 GCAATG GCAATG(2/0)
BsrFI 659 GCCGGC [GA]^CCGG[CT]
BstYI 998 AGATCT [GA]^GATC[CT]
CelII 339 GCTCAGC GC^T[ACGT]AGC
Cfr10I 659 GCCGGC [GA]^CCGG[CT]
EaeI 637 CGGCCA [CT]^GGCC[GA]
Eam1105I 700 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 107 AGCGCC [GA]GCGC^[CT]
HgaI 1058 GCGTC GACGC(5/10)
KspI 325 CCGCGG CCGC^GG
MboII 996 GAAGA GAAGA(8/7)
MflI 998 AGATCT [GA]^GATC[CT]
NaeI 659 GCCGGC GCC^GGC
NcoI 228 CCATGG C^CATGG
NgoMIV 659 GCCGGC G^CCGGC
PflFI 71 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 228 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 378 CTGCAG CTGCA^G
SacII 325 CCGCGG CCGC^GG
SfcI 378 CTGCAG C^T[GA][CT]AG
SfiI 244 GGCCACAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 688 TCGA T^CGA
Tsp509I 450 AATT ^AATT
Tth111I 71 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 688 TCGA T^CGA
Van91I 228 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 965 CCACCAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 998 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 326 CGCG CG^CG
917 CGCG
AspHI 223 GTGCTC G[AT]GC[AT]^C
337 GTGCTC
BcnI 635 CCCGG CC^[GC]GG
815 CCGGG
BsaI 949 GAGACC GGTCTC(1/5)
534 GGTCTC
BsiHKAI 223 GTGCTC G[AT]GC[AT]^C
337 GTGCTC
BspMI 200 ACCTGC ACCTGC(4/8)
287 ACCTGC
BstF5I 747 CATCC GGATG(2/0)
153 GGATG
BstUI 326 CGCG CG^CG
917 CGCG
DraII 467 AGGACCC [GA]G^G[ACGT]CC[CT]
1032 GGGACCC
EcoO109I 467 AGGACCC [GA]G^G[ACGT]CC[CT]
1032 GGGACCC
FokI 747 CATCC GGATG(9/13)
153 GGATG
HinfI 277 GACTC G^A[ACGT]TC
706 GAGTC
MslI 128 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
597 CACCATTGTG
MvnI 326 CGCG CG^CG
917 CGCG
NciI 635 CCCGG CC^[GC]GG
815 CCGGG
PleI 277 GACTC GAGTC(4/5)
706 GAGTC
PpuMI 467 AGGACCC [GA]G^G[AT]CC[CT]
1032 GGGACCC
SmlI 478 CTCAAG C^T[CT][GA]AG
718 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI