Restriction map for
pF1KB5349
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII CfoI ClaI
CpoI DraI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MluNI MroI MscI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 507 CGCG CG^CG
AfaI 10 GTAC GT^AC
AflIII 506 ACGCGT A^C[GA][CT]GT
Alw44I 1068 GTGCAC G^TGCAC
AocI 672 CCTGAGG CC^T[ACGT]AGG
ApaI 43 GGGCCC GGGCC^C
ApaLI 1068 GTGCAC G^TGCAC
Asp700I 232 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 138 GGATCC G^GATCC
BanI 65 GGCACC G^G[CT][GA]CC
BbsI 753 GAAGAC GAAGAC(2/6)
BfaI 604 CTAG C^TAG
BpuAI 753 GAAGAC GAAGAC(2/6)
BsaAI 244 CACGTA [CT]AC^GT[GA]
BsaI 163 GGTCTC GGTCTC(1/5)
BsmAI 164 GTCTC GTCTC(1/5)
BspMI 852 ACCTGC ACCTGC(4/8)
BsrBI 736 GAGCGG CCGCTC(-3/-3)
BsrFI 686 ACCGGC [GA]^CCGG[CT]
BstAPI 654 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 507 CGCG CG^CG
BstXI 153 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 672 CCTGAGG CC^T[ACGT]AGG
Cfr10I 686 ACCGGC [GA]^CCGG[CT]
DrdI 982 GACAAACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1080 GAAGAG CTCTTC(1/4)
Eco81I 672 CCTGAGG CC^T[ACGT]AGG
EcoT14I 153 CCAAGG C^C[AT][AT]GG
FauI 55 CCCGC CCCGC(4/6)
HgaI 508 GCGTC GACGC(5/10)
HincII 547 GTTGAC GT[CT]^[GA]AC
HindII 547 GTTGAC GT[CT]^[GA]AC
HindIII 172 AAGCTT A^AGCTT
Ksp632I 1080 GAAGAG CTCTTC(1/4)
MaeI 604 CTAG C^TAG
MluI 506 ACGCGT A^CGCGT
MvnI 507 CGCG CG^CG
NheI 603 GCTAGC G^CTAGC
PpuMI 377 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 106 CAGCTG CAG^CTG
RsaI 10 GTAC GT^AC
SapI 1080 GAAGAGC GCTCTTC(1/4)
SmaI 46 CCCGGG CCC^GGG
StyI 153 CCAAGG C^C[AT][AT]GG
TfiI 213 GAATC G^A[AT]TC
Tsp45I 442 GTGAC ^GT[GC]AC
XmaCI 46 CCCGGG C^CCGGG
XmaI 46 CCCGGG C^CCGGG
XmnI 232 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 196 GAATTT [GA]^AATT[CT]
812 AAATTC
ApoI 196 GAATTT [GA]^AATT[CT]
812 AAATTC
AvaI 46 CCCGGG C^[CT]CG[GA]G
1013 CCCGAG
AvaII 378 GGACC G^G[AT]CC
1050 GGACC
BseRI 646 CTCCTC GAGGAG(10/8)
76 GAGGAG
BsgI 133 GTGCAG GTGCAG(16/14)
1006 GTGCAG
BsoBI 46 CCCGGG C^[CT]CG[GA]G
1013 CCCGAG
BsrI 476 CCAGT ACTGG(1/-1)
565 CCAGT
BssSI 226 CTCGTG CACGAG(-5/-1)
299 CTCGTG
BstF5I 1119 CATCC GGATG(2/0)
1074 GGATG
BstYI 138 GGATCC [GA]^GATC[CT]
558 GGATCT
DraII 377 GGGACCT [GA]G^G[ACGT]CC[CT]
938 AGGCCCT
DraIII 205 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
655 CACCCTGTG
EcoO109I 377 GGGACCT [GA]G^G[ACGT]CC[CT]
938 AGGCCCT
FokI 1119 CATCC GGATG(9/13)
1074 GGATG
MaeIII 388 GTTAC ^GT[ACGT]AC
442 GTGAC
MfeI 350 CAATTG C^AATTG
401 CAATTG
MflI 138 GGATCC [GA]^GATC[CT]
558 GGATCT
MspA1I 56 CCGCTG C[AC]G^C[GT]G
106 CAGCTG
MunI 350 CAATTG C^AATTG
401 CAATTG
SfcI 22 CTACAG C^T[GA][CT]AG
382 CTACAG
XhoII 138 GGATCC [GA]^GATC[CT]
558 GGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI SacI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp509I TspRI