Restriction map for
pF1KE0611
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MseI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 275 GGTACC G^GTACC
AccI 235 GTAGAC GT^[AC][GT]AC
AciI 470 GCGG CCGC(-3/-1)
AgeI 377 ACCGGT A^CCGGT
AluI 463 AGCT AG^CT
AlwNI 58 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 375 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 275 GGTACC G^GTACC
AspHI 462 GAGCTC G[AT]GC[AT]^C
BalI 194 TGGCCA TGG^CCA
BanI 275 GGTACC G^G[CT][GA]CC
BanII 462 GAGCTC G[GA]GC[CT]^C
BbsI 73 GTCTTC GAAGAC(2/6)
BbvI 428 GCAGC GCAGC(8/12)
BcgI 244 GCAGGCAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 344 CTAG C^TAG
BglI 187 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 462 GAGCTC G[AGT]GC[ACT]^C
BpuAI 73 GTCTTC GAAGAC(2/6)
BsaI 124 GAGACC GGTCTC(1/5)
BsiHKAI 462 GAGCTC G[AT]GC[AT]^C
BsmFI 356 GGGAC GGGAC(10/14)
Bsp1286I 462 GAGCTC G[AGT]GC[ACT]^C
BspMI 69 GCAGGT ACCTGC(4/8)
BsrFI 377 ACCGGT [GA]^CCGG[CT]
BstF5I 183 GGATG GGATG(2/0)
Cac8I 244 GCAGGC GC[ACGT]^[ACGT]GC
Cfr10I 377 ACCGGT [GA]^CCGG[CT]
DraII 472 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 194 TGGCCA [CT]^GGCC[GA]
EcoO109I 472 GGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 470 GCGGG CCCGC(4/6)
Fnu4HI 428 GCAGC GC^[ACGT]GC
FokI 183 GGATG GGATG(9/13)
HgaI 175 GACGC GACGC(5/10)
HphI 155 TCACC GGTGA(8/7)
ItaI 428 GCAGC GC^[ACGT]GC
KpnI 275 GGTACC GGTAC^C
MaeI 344 CTAG C^TAG
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
PinAI 377 ACCGGT A^CCGGT
PpuMI 472 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 462 GAGCTC GAGCT^C
TaqI 383 TCGA T^CGA
TseI 428 GCAGC G^C[AT]GC
TspRI 453 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 383 TCGA T^CGA
XbaI 343 TCTAGA T^CTAGA
XmnI 375 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 84 GTAC GT^AC
276 GTAC
AlwI 228 GATCC GGATC(4/5)
266 GATCC
AvaI 137 CTCGGG C^[CT]CG[GA]G
465 CTCGGG
AvaII 357 GGACC G^G[AT]CC
473 GGTCC
BcnI 293 CCGGG CC^[GC]GG
300 CCGGG
BglII 92 AGATCT A^GATCT
133 AGATCT
BpmI 411 CTCCAG CTGGAG(16/14)
64 CTGGAG
BsaWI 377 ACCGGT [AT]^CCGG[AT]
458 ACCGGA
BseRI 347 GAGGAG GAGGAG(10/8)
350 GAGGAG
BsoBI 137 CTCGGG C^[CT]CG[GA]G
465 CTCGGG
BstNI 240 CCAGG CC^[AT]GG
389 CCAGG
DdeI 37 CTGAG C^T[ACGT]AG
179 CTAAG
EcoRII 240 CCAGG ^CC[AT]GG
389 CCAGG
HinfI 385 GAGTC G^A[ACGT]TC
435 GAGTC
MboII 74 TCTTC GAAGA(8/7)
308 TCTTC
MvaI 240 CCAGG CC^[AT]GG
389 CCAGG
NciI 293 CCGGG CC^[GC]GG
300 CCGGG
NlaIII 189 CATG CATG^
198 CATG
PleI 385 GAGTC GAGTC(4/5)
435 GAGTC
RsaI 84 GTAC GT^AC
276 GTAC
SfaNI 184 GATGC GCATC(5/9)
4 GCATC
Tsp45I 154 GTCAC ^GT[GC]AC
455 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
BsaJI BsiYI BslI BsmAI BssKI
BstYI Cfr13I DpnII DsaI HaeIII
HapII HpaII MaeIII MboI MflI
MnlI MspI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfcI XhoII