Restriction map for
pF1KE0214
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI AviII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 258 GTCTAC GT^[AC][GT]AC
AccII 1026 CGCG CG^CG
AciI 1025 CCGC CCGC(-3/-1)
AgeI 58 ACCGGT A^CCGGT
AlwNI 1223 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 240 ATTAAT AT^TAAT
AsnI 240 ATTAAT AT^TAAT
Asp700I 967 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 329 GTGCTC G[AT]GC[AT]^C
AvrII 936 CCTAGG C^CTAGG
BbsI 318 GTCTTC GAAGAC(2/6)
BbvI 164 GCAGC GCAGC(8/12)
BlnI 936 CCTAGG C^CTAGG
BmyI 329 GTGCTC G[AGT]GC[ACT]^C
BpuAI 318 GTCTTC GAAGAC(2/6)
BsaWI 58 ACCGGT [AT]^CCGG[AT]
BseRI 1184 GAGGAG GAGGAG(10/8)
BsiHKAI 329 GTGCTC G[AT]GC[AT]^C
BsmBI 765 GAGACG CGTCTC(1/5)
Bsp1286I 329 GTGCTC G[AGT]GC[ACT]^C
BspMI 21 ACCTGC ACCTGC(4/8)
BsrDI 828 CATTGC GCAATG(2/0)
BsrFI 58 ACCGGT [GA]^CCGG[CT]
BstBI 500 TTCGAA TT^CGAA
BstUI 1026 CGCG CG^CG
BstXI 865 CCACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 657 AGATCC [GA]^GATC[CT]
Cac8I 448 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 58 ACCGGT [GA]^CCGG[CT]
EcoT14I 936 CCTAGG C^C[AT][AT]GG
Fnu4HI 164 GCAGC GC^[ACGT]GC
HaeIII 981 GGCC GG^CC
HapII 59 CCGG C^CGG
HpaII 59 CCGG C^CGG
HphI 862 TCACC GGTGA(8/7)
ItaI 164 GCAGC GC^[ACGT]GC
MaeII 210 ACGT A^CGT
MflI 657 AGATCC [GA]^GATC[CT]
MslI 1080 CACTATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1317 CAGCTG C[AC]G^C[GT]G
MspI 59 CCGG C^CGG
MvnI 1026 CGCG CG^CG
NdeI 976 CATATG CA^TATG
NspI 737 ACATGC [GA]CATG^[CT]
NspV 500 TTCGAA TT^CGAA
PinAI 58 ACCGGT A^CCGGT
PleI 1187 GAGTC GAGTC(4/5)
PshBI 240 ATTAAT AT^TAAT
PvuII 1317 CAGCTG CAG^CTG
SfuI 500 TTCGAA TT^CGAA
SpeI 928 ACTAGT A^CTAGT
SspI 136 AATATT AAT^ATT
StyI 936 CCTAGG C^C[AT][AT]GG
TseI 164 GCAGC G^C[AT]GC
XhoII 657 AGATCC [GA]^GATC[CT]
XmnI 967 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1144 GGTACC G^GTACC
1238 GGTACC
AcsI 301 AAATTC [GA]^AATT[CT]
1214 GAATTT
AlwI 658 GATCC GGATC(4/5)
898 GGATC
ApoI 301 AAATTC [GA]^AATT[CT]
1214 GAATTT
Asp718I 1144 GGTACC G^GTACC
1238 GGTACC
AvaII 61 GGTCC G^G[AT]CC
1106 GGTCC
BclI 285 TGATCA T^GATCA
468 TGATCA
BsmAI 765 GAGAC GTCTC(1/5)
615 GTCTC
BsmI 750 GAATGC GAATGC(1/-1)
178 GCATTC
Cfr13I 61 GGTCC G^G[ACGT]CC
1106 GGTCC
FbaI 285 TGATCA T^GATCA
468 TGATCA
KpnI 1144 GGTACC GGTAC^C
1238 GGTACC
PflMI 341 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
434 CCACATTTTGG
PstI 220 CTGCAG CTGCA^G
1193 CTGCAG
Sau96I 61 GGTCC G^G[ACGT]CC
1106 GGTCC
SfaNI 1369 GATGC GCATC(5/9)
397 GCATC
SfcI 220 CTGCAG C^T[GA][CT]AG
1193 CTGCAG
TaqI 501 TCGA T^CGA
1132 TCGA
TfiI 859 GATTC G^A[AT]TC
1357 GAATC
Tsp45I 207 GTCAC ^GT[GC]AC
1189 GTCAC
TthHB8I 501 TCGA T^CGA
1132 TCGA
Van91I 341 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
434 CCACATTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BanI BfaI BsaJI
BsiYI BslI BsrI BssKI BstNI
DdeI DpnII EcoRII HinfI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI Tru9I Tsp509I
TspRI