Restriction map for
pF1KE2155
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# NO CUT ENZYMES ( 142 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MfeI MflI MluI MroI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StyI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 925 GACGTC GACGT^C
BbvCI 1038 CCTCAGC CCTCAGC(-5/-2)
BciVI 548 GGATAC GTATCC(6/5)
BclI 791 TGATCA T^GATCA
BfaI 553 CTAG C^TAG
BglI 215 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 463 TACGTG [CT]AC^GT[GA]
BsaBI 681 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1111 GAGACC GGTCTC(1/5)
BsmFI 700 GTCCC GGGAC(10/14)
Bsp1407I 584 TGTACA T^GTACA
BspMI 287 ACCTGC ACCTGC(4/8)
BsrDI 224 GCAATG GCAATG(2/0)
BsrGI 584 TGTACA T^GTACA
BstAPI 615 GCAAGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 166 TTTAAA TTT^AAA
DraII 1025 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1025 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 506 CCCGC CCCGC(4/6)
FbaI 791 TGATCA T^GATCA
HaeII 1085 GGCGCT [GA]GCGC^[CT]
HinfI 133 GAGTC G^A[ACGT]TC
MaeI 553 CTAG C^TAG
MamI 681 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 167 TTAA T^TAA
PleI 133 GAGTC GAGTC(4/5)
PpuMI 1025 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 826 CAGCTG CAG^CTG
SapI 1017 GCTCTTC GCTCTTC(1/4)
SmlI 265 CTCAAG C^T[CT][GA]AG
SphI 428 GCATGC GCATG^C
SspBI 584 TGTACA T^GTACA
StuI 653 AGGCCT AGG^CCT
TaqI 131 TCGA T^CGA
Tru9I 167 TTAA T^TAA
TthHB8I 131 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 351 CGCG CG^CG
508 CGCG
AcyI 925 GACGTC G[GA]^CG[CT]C
988 GGCGTC
BanII 577 GAGCTC G[GA]GC[CT]^C
895 GAGCTC
BpmI 595 CTCCAG CTGGAG(16/14)
751 CTCCAG
BsaHI 925 GACGTC G[GA]^CG[CT]C
988 GGCGTC
BseRI 1089 CTCCTC GAGGAG(10/8)
600 GAGGAG
BsrI 219 ACTGG ACTGG(1/-1)
881 CCAGT
BstUI 351 CGCG CG^CG
508 CGCG
BstXI 665 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1094 CCATGAGTGTGG
EcoNI 747 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1038 CCTCAGCCAGG
HapII 1056 CCGG C^CGG
1083 CCGG
Hin1I 925 GACGTC G[GA]^CG[CT]C
988 GGCGTC
HincII 240 GTTGAC GT[CT]^[GA]AC
961 GTCAAC
HindII 240 GTTGAC GT[CT]^[GA]AC
961 GTCAAC
HpaII 1056 CCGG C^CGG
1083 CCGG
MspA1I 826 CAGCTG C[AC]G^C[GT]G
951 CCGCTG
MspI 1056 CCGG C^CGG
1083 CCGG
MvnI 351 CGCG CG^CG
508 CGCG
NlaIV 176 GGTTCC GG[ACGT]^[ACGT]CC
699 GGTCCC
NspI 428 GCATGC [GA]CATG^[CT]
645 GCATGT
PflMI 356 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCAGACGGTGG
SacI 577 GAGCTC GAGCT^C
895 GAGCTC
SfcI 345 CTACAG C^T[GA][CT]AG
933 CTACAG
Van91I 356 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCAGACGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BbsI BbvI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI