Restriction map for
pF1KE1099
##################################
# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FauI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AciI 247 GCGG CCGC(-3/-1)
AcsI 885 AAATTC [GA]^AATT[CT]
AcyI 737 GGCGTC G[GA]^CG[CT]C
AlwNI 725 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 892 CCTGAGG CC^T[ACGT]AGG
ApoI 885 AAATTC [GA]^AATT[CT]
Asp700I 295 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 820 GGACC G^G[AT]CC
BbsI 740 GTCTTC GAAGAC(2/6)
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
BclI 519 TGATCA T^GATCA
BpmI 572 CTCCAG CTGGAG(16/14)
BpuAI 740 GTCTTC GAAGAC(2/6)
BsaHI 737 GGCGTC G[GA]^CG[CT]C
BsiYI 309 CCTGTGGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 309 CCTGTGGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI 162 GCAGGT ACCTGC(4/8)
BsrDI 52 GCAATG GCAATG(2/0)
BstAPI 162 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 711 GGATG GGATG(2/0)
Bsu36I 892 CCTGAGG CC^T[ACGT]AGG
Cac8I 158 GCTTGC GC[ACGT]^[ACGT]GC
DraI 832 TTTAAA TTT^AAA
Eco81I 892 CCTGAGG CC^T[ACGT]AGG
EcoRV 469 GATATC GAT^ATC
EcoT14I 223 CCTTGG C^C[AT][AT]GG
FbaI 519 TGATCA T^GATCA
FokI 711 GGATG GGATG(9/13)
HaeIII 367 GGCC GG^CC
HapII 439 CCGG C^CGG
Hin1I 737 GGCGTC G[GA]^CG[CT]C
HpaII 439 CCGG C^CGG
MaeII 243 ACGT A^CGT
MslI 861 CATACTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 728 CAGCTG C[AC]G^C[GT]G
MspI 439 CCGG C^CGG
NlaIV 570 GGCTCC GG[ACGT]^[ACGT]CC
NspI 430 GCATGT [GA]CATG^[CT]
PleI 898 GAGTC GAGTC(4/5)
PvuII 728 CAGCTG CAG^CTG
ScaI 550 AGTACT AGT^ACT
SfaNI 168 GATGC GCATC(5/9)
SfcI 663 CTACAG C^T[GA][CT]AG
SmlI 265 CTTGAG C^T[CT][GA]AG
SpeI 864 ACTAGT A^CTAGT
SspI 105 AATATT AAT^ATT
StyI 223 CCTTGG C^C[AT][AT]GG
TfiI 810 GATTC G^A[AT]TC
XmnI 295 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 191 GTAC GT^AC
551 GTAC
BsaJI 186 CCCAGG C^C[ACGT][ACGT]GG
223 CCTTGG
BseRI 73 GAGGAG GAGGAG(10/8)
895 GAGGAG
BsmFI 11 GGGAC GGGAC(10/14)
584 GTCCC
BsmI 216 GAATGC GAATGC(1/-1)
527 GCATTC
BsrI 210 CCAGT ACTGG(1/-1)
574 CCAGT
BssKI 187 CCAGG ^CC[ACGT]GG
364 CCAGG
BstNI 187 CCAGG CC^[AT]GG
364 CCAGG
Cfr13I 367 GGCCC G^G[ACGT]CC
820 GGACC
EarI 391 GAAGAG CTCTTC(1/4)
945 GAAGAG
EcoRII 187 CCAGG ^CC[AT]GG
364 CCAGG
HgaI 13 GACGC GACGC(5/10)
738 GCGTC
HinfI 810 GATTC G^A[ACGT]TC
898 GAGTC
Ksp632I 391 GAAGAG CTCTTC(1/4)
945 GAAGAG
MvaI 187 CCAGG CC^[AT]GG
364 CCAGG
RsaI 191 GTAC GT^AC
551 GTAC
Sau96I 367 GGCCC G^G[ACGT]CC
820 GGACC
ScrFI 187 CCAGG CC^[ACGT]GG
364 CCAGG
TspRI 474 CAGTG CA[GC]TG[ACGT][ACGT]^
575 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BbvI BfaI DdeI DpnII
Fnu4HI HphI ItaI MaeI MboI
MboII MnlI MseI MwoI NdeII
NlaIII Sau3AI Tru9I TseI Tsp509I