Restriction map for
pF1KB6214
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstUI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KspI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1368 GGTACC G^GTACC
AocI 391 CCTCAGG CC^T[ACGT]AGG
ApaI 193 GGGCCC GGGCC^C
Asp700I 568 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1368 GGTACC G^GTACC
AspHI 62 GTGCTC G[AT]GC[AT]^C
AvaI 422 CTCGGG C^[CT]CG[GA]G
BanI 1368 GGTACC G^G[CT][GA]CC
BanII 193 GGGCCC G[GA]GC[CT]^C
BbsI 1188 GTCTTC GAAGAC(2/6)
BbvI 714 GCAGC GCAGC(8/12)
BpmI 96 CTCCAG CTGGAG(16/14)
BpuAI 1188 GTCTTC GAAGAC(2/6)
BsaBI 1915 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1531 GAGACC GGTCTC(1/5)
BsaWI 553 ACCGGA [AT]^CCGG[AT]
BsgI 2014 GTGCAG GTGCAG(16/14)
BsiHKAI 62 GTGCTC G[AT]GC[AT]^C
BsmI 557 GAATGC GAATGC(1/-1)
BsoBI 422 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1104 TGTACA T^GTACA
BspHI 1137 TCATGA T^CATGA
BspMI 914 GCAGGT ACCTGC(4/8)
BsrGI 1104 TGTACA T^GTACA
BssSI 152 CTCGTG CACGAG(-5/-1)
BstEII 1897 GGTAACC G^GT[ACGT]ACC
BstPI 1897 GGTAACC G^GT[ACGT]ACC
BstXI 1583 CCACCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 391 CCTCAGG CC^T[ACGT]AGG
DraIII 722 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 738 GACCCGTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 391 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1897 GGTAACC G^GT[ACGT]ACC
Fnu4HI 714 GCAGC GC^[ACGT]GC
HapII 554 CCGG C^CGG
HincII 28 GTCAAC GT[CT]^[GA]AC
HindII 28 GTCAAC GT[CT]^[GA]AC
HindIII 1046 AAGCTT A^AGCTT
HpaII 554 CCGG C^CGG
ItaI 714 GCAGC GC^[ACGT]GC
KpnI 1368 GGTACC GGTAC^C
MaeII 1245 ACGT A^CGT
MamI 1915 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1733 CAGCTG C[AC]G^C[GT]G
MspI 554 CCGG C^CGG
NcoI 1892 CCATGG C^CATGG
NdeI 803 CATATG CA^TATG
PpuMI 736 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1733 CAGCTG CAG^CTG
RcaI 1137 TCATGA T^CATGA
ScaI 1879 AGTACT AGT^ACT
SspBI 1104 TGTACA T^GTACA
TseI 714 GCAGC G^C[AT]GC
XmnI 568 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1333 AAATTT [GA]^AATT[CT]
1560 AAATTC
ApoI 1333 AAATTT [GA]^AATT[CT]
1560 AAATTC
AvaII 243 GGACC G^G[AT]CC
737 GGACC
BmyI 62 GTGCTC G[AGT]GC[ACT]^C
193 GGGCCC
BseRI 1644 CTCCTC GAGGAG(10/8)
1653 CTCCTC
BsmFI 736 GGGAC GGGAC(10/14)
430 GTCCC
Bsp1286I 62 GTGCTC G[AGT]GC[ACT]^C
193 GGGCCC
BsrDI 533 CATTGC GCAATG(2/0)
1930 GCAATG
DpnII 309 GATC ^GATC
1915 GATC
DsaI 660 CCGTGG C^C[GA][CT]GG
1892 CCATGG
EcoT14I 1705 CCAAGG C^C[AT][AT]GG
1892 CCATGG
MboI 309 GATC ^GATC
1915 GATC
MwoI 582 GCCTCTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
760 GCCAAGCCTGC
NdeII 309 GATC ^GATC
1915 GATC
NspI 1055 ACATGC [GA]CATG^[CT]
1858 ACATGC
PflMI 234 CCATCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
802 CCATATGATGG
PstI 221 CTGCAG CTGCA^G
1211 CTGCAG
Sau3AI 309 GATC ^GATC
1915 GATC
SfaNI 1078 GATGC GCATC(5/9)
78 GCATC
SspI 1519 AATATT AAT^ATT
1671 AATATT
StyI 1705 CCAAGG C^C[AT][AT]GG
1892 CCATGG
Tsp45I 550 GTGAC ^GT[GC]AC
1167 GTCAC
Van91I 234 CCATCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
802 CCATATGATGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI BciVI BfaI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EarI EcoO109I EcoRII FokI
HaeIII HinfI HphI Ksp632I MaeI
MaeIII MboII MnlI MseI MslI
MvaI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfcI SmlI TfiI
Tru9I Tsp509I TspRI