Restriction map for
pF1KB6595
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BbvI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII CfoI ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I FauI FbaI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
Ksp632I KspI MaeI MamI MluI
MluNI MroI MscI MspA1I MvnI
MwoI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TseI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 718 CCGC CCGC(-3/-1)
AgeI 1075 ACCGGT A^CCGGT
AocI 103 CCTCAGG CC^T[ACGT]AGG
AvaI 740 CTCGGG C^[CT]CG[GA]G
AvaII 787 GGACC G^G[AT]CC
BamHI 123 GGATCC G^GATCC
BcgI 904 CGACAATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 130 GGATAC GTATCC(6/5)
BcnI 127 CCCGG CC^[GC]GG
BsaWI 1075 ACCGGT [AT]^CCGG[AT]
BseRI 101 CTCCTC GAGGAG(10/8)
BsmAI 171 GTCTC GTCTC(1/5)
BsmFI 786 GGGAC GGGAC(10/14)
BsmI 190 GAATGC GAATGC(1/-1)
BsoBI 740 CTCGGG C^[CT]CG[GA]G
BsrFI 1075 ACCGGT [GA]^CCGG[CT]
Bsu36I 103 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1075 ACCGGT [GA]^CCGG[CT]
Cfr13I 787 GGACC G^G[ACGT]CC
DraII 786 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 328 CCATGG C^C[GA][CT]GG
Eco81I 103 CCTCAGG CC^T[ACGT]AGG
EcoNI 475 CCTGGCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 786 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 401 GAATTC G^AATTC
EcoRV 387 GATATC GAT^ATC
EcoT22I 539 ATGCAT ATGCA^T
Fnu4HI 717 GCCGC GC^[ACGT]GC
HincII 94 GTTAAC GT[CT]^[GA]AC
HindII 94 GTTAAC GT[CT]^[GA]AC
HpaI 94 GTTAAC GTT^AAC
ItaI 717 GCCGC GC^[ACGT]GC
MaeII 1026 ACGT A^CGT
MfeI 444 CAATTG C^AATTG
MunI 444 CAATTG C^AATTG
NciI 127 CCCGG CC^[GC]GG
NcoI 328 CCATGG C^CATGG
NsiI 539 ATGCAT ATGCA^T
PinAI 1075 ACCGGT A^CCGGT
PpuMI 786 GGGACCT [GA]G^G[AT]CC[CT]
Sau96I 787 GGACC G^G[ACGT]CC
SfcI 857 CTACAG C^T[GA][CT]AG
TaqI 1009 TCGA T^CGA
Tsp45I 850 GTGAC ^GT[GC]AC
TthHB8I 1009 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 65 GTAC GT^AC
487 GTAC
AluI 692 AGCT AG^CT
764 AGCT
BbsI 243 GAAGAC GAAGAC(2/6)
1000 GAAGAC
BpuAI 243 GAAGAC GAAGAC(2/6)
1000 GAAGAC
BsiYI 99 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
475 CCTGGCATAGG
BslI 99 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
475 CCTGGCATAGG
BsrDI 153 GCAATG GCAATG(2/0)
333 GCAATG
BstF5I 25 GGATG GGATG(2/0)
586 GGATG
BstNI 365 CCTGG CC^[AT]GG
475 CCTGG
BstYI 123 GGATCC [GA]^GATC[CT]
670 AGATCC
EcoRII 365 CCTGG ^CC[AT]GG
475 CCTGG
EcoT14I 328 CCATGG C^C[AT][AT]GG
845 CCTTGG
FokI 25 GGATG GGATG(9/13)
586 GGATG
HapII 128 CCGG C^CGG
1076 CCGG
HinfI 261 GAATC G^A[ACGT]TC
1116 GATTC
HpaII 128 CCGG C^CGG
1076 CCGG
MaeIII 850 GTGAC ^GT[ACGT]AC
1072 GTAAC
MflI 123 GGATCC [GA]^GATC[CT]
670 AGATCC
MslI 329 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
542 CATTACAGTG
MspI 128 CCGG C^CGG
1076 CCGG
MvaI 365 CCTGG CC^[AT]GG
475 CCTGG
NlaIV 123 GGATCC GG[ACGT]^[ACGT]CC
786 GGGACC
RsaI 65 GTAC GT^AC
487 GTAC
StyI 328 CCATGG C^C[AT][AT]GG
845 CCTTGG
TfiI 261 GAATC G^A[AT]TC
1116 GATTC
XhoII 123 GGATCC [GA]^GATC[CT]
670 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AcsI AlwI ApoI BsaJI BsrI
BssKI DdeI DpnII HphI MboI
MboII MnlI MseI NdeII NlaIII
Sau3AI ScrFI SfaNI SspI Tru9I
Tsp509I TspRI