Restriction map for
pF1KE2761
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspHI AspI AviII AvrII BalI
BamHI BbeI BcgI BciVI BfrI
BglI BlnI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 544 GGTACC G^GTACC
AccII 679 CGCG CG^CG
AflIII 104 ACATGT A^C[GA][CT]GT
AhdI 2420 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 810 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 544 GGTACC G^GTACC
AspEI 2420 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 2055 GGGCTC G[GA]GC[CT]^C
BbrPI 1631 CACGTG CAC^GTG
BbvCI 2278 CCTCAGC CCTCAGC(-5/-2)
BclI 944 TGATCA T^GATCA
BcnI 2345 CCCGG CC^[GC]GG
BglII 125 AGATCT A^GATCT
BmyI 2055 GGGCTC G[AGT]GC[ACT]^C
BpmI 1525 CTCCAG CTGGAG(16/14)
BsaAI 1631 CACGTG [CT]AC^GT[GA]
BsgI 2223 CTGCAC GTGCAG(16/14)
Bsp1286I 2055 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 104 ACATGT A^CATGT
BspMI 1917 ACCTGC ACCTGC(4/8)
BsrDI 2124 CATTGC GCAATG(2/0)
BstAPI 421 GCAAAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 17 TTCGAA TT^CGAA
BstUI 679 CGCG CG^CG
BstYI 125 AGATCT [GA]^GATC[CT]
DraII 492 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2420 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 492 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 944 TGATCA T^GATCA
HapII 2346 CCGG C^CGG
HpaII 2346 CCGG C^CGG
KpnI 544 GGTACC GGTAC^C
MfeI 1098 CAATTG C^AATTG
MflI 125 AGATCT [GA]^GATC[CT]
MspA1I 2220 CAGCTG C[AC]G^C[GT]G
MspI 2346 CCGG C^CGG
MunI 1098 CAATTG C^AATTG
MvnI 679 CGCG CG^CG
NciI 2345 CCCGG CC^[GC]GG
NspV 17 TTCGAA TT^CGAA
PmaCI 1631 CACGTG CAC^GTG
PmlI 1631 CACGTG CAC^GTG
PpuMI 492 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1646 CTGCAG CTGCA^G
PvuII 2220 CAGCTG CAG^CTG
ScaI 1281 AGTACT AGT^ACT
SfuI 17 TTCGAA TT^CGAA
SmlI 303 CTCAAG C^T[CT][GA]AG
SphI 2317 GCATGC GCATG^C
SspI 466 AATATT AAT^ATT
XhoII 125 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 678 CCGC CCGC(-3/-1)
2114 CCGC
AlwI 826 GATCC GGATC(4/5)
259 GGATC
AvaI 668 CTCGGG C^[CT]CG[GA]G
716 CCCGAG
BanI 544 GGTACC G^G[CT][GA]CC
1573 GGTGCC
BbsI 2496 GAAGAC GAAGAC(2/6)
1625 GTCTTC
BlpI 702 GCTCAGC GC^T[ACGT]AGC
1417 GCTAAGC
Bpu1102I 702 GCTCAGC GC^T[ACGT]AGC
1417 GCTAAGC
BpuAI 2496 GAAGAC GAAGAC(2/6)
1625 GTCTTC
BseRI 833 CTCCTC GAGGAG(10/8)
1257 CTCCTC
BsmI 20 GAATGC GAATGC(1/-1)
1359 GAATGC
BsoBI 668 CTCGGG C^[CT]CG[GA]G
716 CCCGAG
BspHI 623 TCATGA T^CATGA
1461 TCATGA
CelII 702 GCTCAGC GC^T[ACGT]AGC
1417 GCTAAGC
DsaI 1802 CCATGG C^C[GA][CT]GG
2063 CCATGG
EcoT22I 22 ATGCAT ATGCA^T
207 ATGCAT
HgaI 1760 GACGC GACGC(5/10)
1623 GCGTC
HincII 251 GTCAAC GT[CT]^[GA]AC
2032 GTCAAC
HindII 251 GTCAAC GT[CT]^[GA]AC
2032 GTCAAC
HindIII 368 AAGCTT A^AGCTT
1504 AAGCTT
HphI 1287 GGTGA GGTGA(8/7)
2111 TCACC
NcoI 1802 CCATGG C^CATGG
2063 CCATGG
NsiI 22 ATGCAT ATGCA^T
207 ATGCAT
RcaI 623 TCATGA T^CATGA
1461 TCATGA
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
AvaII BbvI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BssSI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I