Restriction map for
pF1KE4516
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI BstAPI BstBI CelII
ClaI CpoI DraI DraIII Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1580 CGCG CG^CG
AcyI 740 GGCGCC G[GA]^CG[CT]C
AocI 852 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1492 AGCGCT AGC^GCT
AviII 532 TGCGCA TGC^GCA
BbeI 740 GGCGCC GGCGC^C
BciVI 1344 GTATCC GTATCC(6/5)
BsaHI 740 GGCGCC G[GA]^CG[CT]C
BsiEI 39 CGGCCG CG[GA][CT]^CG
Bsp1407I 419 TGTACA T^GTACA
BsrFI 1181 GCCGGC [GA]^CCGG[CT]
BsrGI 419 TGTACA T^GTACA
Bst1107I 1329 GTATAC GTA^TAC
BstEII 60 GGTCACC G^GT[ACGT]ACC
BstPI 60 GGTCACC G^GT[ACGT]ACC
BstUI 1580 CGCG CG^CG
BstXI 1142 CCAAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1329 GTATAC GTA^TAC
Bsu36I 852 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1181 GCCGGC [GA]^CCGG[CT]
DrdI 513 GACAGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 39 CGGCCG C^GGCCG
EclXI 39 CGGCCG C^GGCCG
Eco47III 1492 AGCGCT AGC^GCT
Eco52I 39 CGGCCG C^GGCCG
Eco81I 852 CCTGAGG CC^T[ACGT]AGG
EcoO65I 60 GGTCACC G^GT[ACGT]ACC
FspI 532 TGCGCA TGC^GCA
HgaI 1578 GACGC GACGC(5/10)
Hin1I 740 GGCGCC G[GA]^CG[CT]C
HindIII 1144 AAGCTT A^AGCTT
KasI 740 GGCGCC G^GCGCC
MseI 8 TTAA T^TAA
MvnI 1580 CGCG CG^CG
NaeI 1181 GCCGGC GCC^GGC
NarI 740 GGCGCC GG^CGCC
NgoMIV 1181 GCCGGC G^CCGGC
PflMI 1028 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1438 GAGCTC GAGCT^C
SbfI 1077 CCTGCAGG CCTGCA^GG
SfoI 740 GGCGCC GGC^GCC
SmaI 203 CCCGGG CCC^GGG
Sse8387I 1077 CCTGCAGG CCTGCA^GG
SspBI 419 TGTACA T^GTACA
StuI 1622 AGGCCT AGG^CCT
Tru9I 8 TTAA T^TAA
Tsp509I 908 AATT ^AATT
Van91I 1028 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 203 CCCGGG C^CCGGG
XmaI 203 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 685 GTCTAC GT^[AC][GT]AC
1329 GTATAC
ApaI 392 GGGCCC GGGCC^C
857 GGGCCC
BanI 667 GGCACC G^G[CT][GA]CC
740 GGCGCC
BbvCI 168 CCTCAGC CCTCAGC(-5/-2)
1625 CCTCAGC
BfaI 475 CTAG C^TAG
1046 CTAG
BglI 732 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1189 GCCCAGATGGC
BpmI 187 CTGGAG CTGGAG(16/14)
1555 CTGGAG
BsaI 1516 GAGACC GGTCTC(1/5)
759 GGTCTC
BsmAI 1516 GAGAC GTCTC(1/5)
760 GTCTC
BstYI 53 AGATCC [GA]^GATC[CT]
326 GGATCT
EarI 1012 CTCTTC CTCTTC(1/4)
1459 CTCTTC
HaeII 740 GGCGCC [GA]GCGC^[CT]
1492 AGCGCT
Ksp632I 1012 CTCTTC CTCTTC(1/4)
1459 CTCTTC
MaeI 475 CTAG C^TAG
1046 CTAG
MflI 53 AGATCC [GA]^GATC[CT]
326 GGATCT
MslI 63 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1371 CATGGGAATG
PvuII 403 CAGCTG CAG^CTG
515 CAGCTG
XhoII 53 AGATCC [GA]^GATC[CT]
326 GGATCT
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI
XcmI