Restriction map for
pF1KB7832
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRII FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI MvaI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 510 CGCG CG^CG
AfaI 77 GTAC GT^AC
AflIII 916 ACATGT A^C[GA][CT]GT
Alw44I 751 GTGCAC G^TGCAC
AlwNI 220 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1445 CCTCAGG CC^T[ACGT]AGG
ApaLI 751 GTGCAC G^TGCAC
AvaI 703 CTCGAG C^[CT]CG[GA]G
BanII 1453 GAGCTC G[GA]GC[CT]^C
BbvI 292 GCTGC GCAGC(8/12)
BciVI 1740 GGATAC GTATCC(6/5)
BclI 189 TGATCA T^GATCA
BcnI 866 CCGGG CC^[GC]GG
BsmI 615 GAATGC GAATGC(1/-1)
BsoBI 703 CTCGAG C^[CT]CG[GA]G
BspLU11I 916 ACATGT A^CATGT
BspMI 58 GCAGGT ACCTGC(4/8)
BssKI 866 CCGGG ^CC[ACGT]GG
BstF5I 1659 GGATG GGATG(2/0)
BstUI 510 CGCG CG^CG
BstXI 996 CCACTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1445 CCTCAGG CC^T[ACGT]AGG
CfoI 509 GCGC GCG^C
Eco81I 1445 CCTCAGG CC^T[ACGT]AGG
EcoNI 499 CCTAAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 94 GAATTC G^AATTC
EcoRV 384 GATATC GAT^ATC
EcoT14I 893 CCAAGG C^C[AT][AT]GG
FauI 51 GCGGG CCCGC(4/6)
FbaI 189 TGATCA T^GATCA
Fnu4HI 292 GCTGC GC^[ACGT]GC
FokI 1659 GGATG GGATG(9/13)
HapII 866 CCGG C^CGG
HgaI 836 GCGTC GACGC(5/10)
HhaI 509 GCGC GCG^C
HinP1I 509 GCGC G^CGC
HpaII 866 CCGG C^CGG
ItaI 292 GCTGC GC^[ACGT]GC
MspI 866 CCGG C^CGG
MvnI 510 CGCG CG^CG
NciI 866 CCGGG CC^[GC]GG
NlaIV 1472 GGTCCC GG[ACGT]^[ACGT]CC
PaeR7I 703 CTCGAG C^TCGAG
PvuII 1030 CAGCTG CAG^CTG
RsaI 77 GTAC GT^AC
SacI 1453 GAGCTC GAGCT^C
ScrFI 866 CCGGG CC^[ACGT]GG
SphI 1133 GCATGC GCATG^C
StyI 893 CCAAGG C^C[AT][AT]GG
TseI 292 GCTGC G^C[AT]GC
XbaI 1584 TCTAGA T^CTAGA
XhoI 703 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 51 GCGG CCGC(-3/-1)
856 GCGG
AlwI 879 GGATC GGATC(4/5)
1602 GGATC
Asp700I 161 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
549 GAAGGCTTTC
AvaII 1472 GGTCC G^G[AT]CC
1713 GGACC
BbsI 428 GTCTTC GAAGAC(2/6)
1120 GTCTTC
BpmI 1590 CTCCAG CTGGAG(16/14)
1313 CTGGAG
BpuAI 428 GTCTTC GAAGAC(2/6)
1120 GTCTTC
BsaJI 866 CCGGGG C^C[ACGT][ACGT]GG
893 CCAAGG
BsgI 1571 GTGCAG GTGCAG(16/14)
1595 GTGCAG
BsiYI 499 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
565 CCGAAAACAGG
BslI 499 CCTAAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
565 CCGAAAACAGG
BsmFI 1705 GGGAC GGGAC(10/14)
1473 GTCCC
Cfr13I 1472 GGTCC G^G[ACGT]CC
1713 GGACC
EcoT22I 617 ATGCAT ATGCA^T
1135 ATGCAT
HaeIII 126 GGCC GG^CC
897 GGCC
HincII 708 GTTGAC GT[CT]^[GA]AC
1776 GTCAAC
HindII 708 GTTGAC GT[CT]^[GA]AC
1776 GTCAAC
MaeII 777 ACGT A^CGT
989 ACGT
MspA1I 49 CAGCGG C[AC]G^C[GT]G
1030 CAGCTG
NdeI 1241 CATATG CA^TATG
1413 CATATG
NsiI 617 ATGCAT ATGCA^T
1135 ATGCAT
SapI 871 GAAGAGC GCTCTTC(1/4)
1693 GCTCTTC
Sau96I 1472 GGTCC G^G[ACGT]CC
1713 GGACC
XmnI 161 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
549 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI ApoI AspHI BfaI
BmyI BsiHKAI BsmAI Bsp1286I BsrI
Cac8I DdeI DpnII DraI EarI
HinfI HphI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIII NspI PleI
Sau3AI SfaNI SfcI SmlI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I