Restriction map for
pF1KE1046
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BsiEI BsiWI BsiYI
BslI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 225 GACGCC G[GA]^CG[CT]C
AlwI 236 GGATC GGATC(4/5)
AspHI 370 GAGCTC G[AT]GC[AT]^C
AvaI 425 CCCGGG C^[CT]CG[GA]G
AvaII 271 GGACC G^G[AT]CC
BanI 62 GGCACC G^G[CT][GA]CC
BanII 370 GAGCTC G[GA]GC[CT]^C
BbvI 288 GCTGC GCAGC(8/12)
BglI 344 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 225 GACGCC G[GA]^CG[CT]C
BsaWI 29 ACCGGA [AT]^CCGG[AT]
BsiHKAI 370 GAGCTC G[AT]GC[AT]^C
BsmAI 223 GAGAC GTCTC(1/5)
BsmBI 223 GAGACG CGTCTC(1/5)
BsoBI 425 CCCGGG C^[CT]CG[GA]G
BspHI 248 TCATGA T^CATGA
BsrBI 135 CCGCTC CCGCTC(-3/-3)
BstNI 183 CCTGG CC^[AT]GG
CfoI 307 GCGC GCG^C
DpnII 237 GATC ^GATC
DraII 270 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 124 CTCTTC CTCTTC(1/4)
EcoO109I 270 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 183 CCTGG ^CC[AT]GG
EcoT14I 89 CCAAGG C^C[AT][AT]GG
Fnu4HI 288 GCTGC GC^[ACGT]GC
HgaI 225 GACGC GACGC(5/10)
HhaI 307 GCGC GCG^C
Hin1I 225 GACGCC G[GA]^CG[CT]C
HinP1I 307 GCGC G^CGC
HphI 327 GGTGA GGTGA(8/7)
ItaI 288 GCTGC GC^[ACGT]GC
Ksp632I 124 CTCTTC CTCTTC(1/4)
MaeIII 203 GTTAC ^GT[ACGT]AC
MboI 237 GATC ^GATC
MspA1I 346 CCGCTG C[AC]G^C[GT]G
MvaI 183 CCTGG CC^[AT]GG
NdeII 237 GATC ^GATC
PleI 391 GACTC GAGTC(4/5)
PpuMI 270 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 248 TCATGA T^CATGA
SacI 370 GAGCTC GAGCT^C
SapI 123 GCTCTTC GCTCTTC(1/4)
Sau3AI 237 GATC ^GATC
SfaNI 120 GATGC GCATC(5/9)
SmaI 425 CCCGGG CCC^GGG
StyI 89 CCAAGG C^C[AT][AT]GG
TseI 288 GCTGC G^C[AT]GC
XmaCI 425 CCCGGG C^CCGGG
XmaI 425 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 18 GTAC GT^AC
114 GTAC
BcnI 425 CCCGG CC^[GC]GG
426 CCGGG
BfaI 163 CTAG C^TAG
197 CTAG
BmyI 342 GTGCCC G[AGT]GC[ACT]^C
370 GAGCTC
BsaJI 89 CCAAGG C^C[ACGT][ACGT]GG
425 CCCGGG
BseRI 94 GAGGAG GAGGAG(10/8)
97 GAGGAG
BsgI 46 CTGCAC GTGCAG(16/14)
289 CTGCAC
BsmFI 270 GGGAC GGGAC(10/14)
156 GTCCC
Bsp1286I 342 GTGCCC G[AGT]GC[ACT]^C
370 GAGCTC
BsrI 232 ACTGG ACTGG(1/-1)
387 ACTGG
Cfr13I 271 GGACC G^G[ACGT]CC
351 GGGCC
FauI 159 CCCGC CCCGC(4/6)
345 CCCGC
HaeIII 186 GGCC GG^CC
352 GGCC
HapII 30 CCGG C^CGG
426 CCGG
HpaII 30 CCGG C^CGG
426 CCGG
MaeI 163 CTAG C^TAG
197 CTAG
MnlI 94 GAGG CCTC(7/6)
97 GAGG
MspI 30 CCGG C^CGG
426 CCGG
MwoI 344 GCCCGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
363 GCAAAGTGAGC
NciI 425 CCCGG CC^[GC]GG
426 CCGGG
NlaIII 168 CATG CATG^
249 CATG
RsaI 18 GTAC GT^AC
114 GTAC
Sau96I 271 GGACC G^G[ACGT]CC
351 GGGCC
SfcI 51 CTACAG C^T[GA][CT]AG
300 CTACAG
TfiI 84 GATTC G^A[AT]TC
333 GAATC
TspRI 231 CACTG CA[GC]TG[ACGT][ACGT]^
303 CAGTG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AciI AluI BssKI BstF5I Cac8I
FokI HinfI MboII NlaIV ScrFI