Restriction map for
pF1KB7603
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KspI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 877 AAATTC [GA]^AATT[CT]
AflII 13 CTTAAG C^TTAAG
ApaI 97 GGGCCC GGGCC^C
ApoI 877 AAATTC [GA]^AATT[CT]
AspHI 461 GTGCTC G[AT]GC[AT]^C
BalI 408 TGGCCA TGG^CCA
BbsI 363 GAAGAC GAAGAC(2/6)
BfrI 13 CTTAAG C^TTAAG
BpuAI 363 GAAGAC GAAGAC(2/6)
BsaI 348 GAGACC GGTCTC(1/5)
BsiHKAI 461 GTGCTC G[AT]GC[AT]^C
BsmAI 348 GAGAC GTCTC(1/5)
BsrDI 447 CATTGC GCAATG(2/0)
BssSI 870 CTCGTG CACGAG(-5/-1)
BstAPI 187 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 866 CATCC GGATG(2/0)
BstXI 744 CCAGGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 507 AGATCC [GA]^GATC[CT]
EaeI 408 TGGCCA [CT]^GGCC[GA]
EcoT14I 686 CCATGG C^C[AT][AT]GG
EcoT22I 646 ATGCAT ATGCA^T
FauI 115 CCCGC CCCGC(4/6)
FokI 866 CATCC GGATG(9/13)
HaeII 137 AGCGCC [GA]GCGC^[CT]
MaeII 154 ACGT A^CGT
MaeIII 703 GTCAC ^GT[ACGT]AC
MflI 507 AGATCC [GA]^GATC[CT]
MluNI 408 TGGCCA TGG^CCA
MscI 408 TGGCCA TGG^CCA
NcoI 686 CCATGG C^CATGG
NsiI 646 ATGCAT ATGCA^T
NspI 533 GCATGC [GA]CATG^[CT]
PpuMI 541 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 290 GAAGAGC GCTCTTC(1/4)
SfaNI 648 GCATC GCATC(5/9)
SmaI 93 CCCGGG CCC^GGG
SmlI 13 CTTAAG C^T[CT][GA]AG
SphI 533 GCATGC GCATG^C
StyI 686 CCATGG C^C[AT][AT]GG
TfiI 285 GAATC G^A[AT]TC
Tsp45I 703 GTCAC ^GT[GC]AC
XhoII 507 AGATCC [GA]^GATC[CT]
XmaCI 93 CCCGGG C^CCGGG
XmaI 93 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 206 GGTACC G^GTACC
691 GGTACC
AfaI 207 GTAC GT^AC
692 GTAC
AlwI 508 GATCC GGATC(4/5)
628 GGATC
Asp718I 206 GGTACC G^GTACC
691 GGTACC
BanI 206 GGTACC G^G[CT][GA]CC
691 GGTACC
BanII 97 GGGCCC G[GA]GC[CT]^C
480 GAGCCC
BfaI 172 CTAG C^TAG
428 CTAG
BpmI 371 CTCCAG CTGGAG(16/14)
477 CTGGAG
BseRI 522 CTCCTC GAGGAG(10/8)
229 GAGGAG
BsgI 73 CTGCAC GTGCAG(16/14)
268 GTGCAG
BspMI 351 ACCTGC ACCTGC(4/8)
270 GCAGGT
CfoI 138 GCGC GCG^C
166 GCGC
DsaI 83 CCGTGG C^C[GA][CT]GG
686 CCATGG
EarI 525 CTCTTC CTCTTC(1/4)
290 GAAGAG
HhaI 138 GCGC GCG^C
166 GCGC
HinP1I 138 GCGC G^CGC
166 GCGC
HindIII 762 AAGCTT A^AGCTT
823 AAGCTT
HphI 651 TCACC GGTGA(8/7)
704 TCACC
KpnI 206 GGTACC GGTAC^C
691 GGTACC
Ksp632I 525 CTCTTC CTCTTC(1/4)
290 GAAGAG
MaeI 172 CTAG C^TAG
428 CTAG
MslI 216 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
866 CATCCTCGTG
PleI 45 GAGTC GAGTC(4/5)
259 GAGTC
RsaI 207 GTAC GT^AC
692 GTAC
TaqI 262 TCGA T^CGA
288 TCGA
Tsp509I 413 AATT ^AATT
878 AATT
TthHB8I 262 TCGA T^CGA
288 TCGA
XcmI 53 CCACTTCGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
782 CCACTGATTGCTTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tru9I TseI TspRI