Restriction map for
pF1KE2271
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BglI BglII
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MflI MluI MluNI
MroI MscI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 235 CGCG CG^CG
AcsI 972 AAATTT [GA]^AATT[CT]
AfaI 122 GTAC GT^AC
AflII 295 CTTAAG C^TTAAG
AflIII 1360 ACATGT A^C[GA][CT]GT
AgeI 1346 ACCGGT A^CCGGT
AlwI 752 GGATC GGATC(4/5)
AocI 1087 CCTGAGG CC^T[ACGT]AGG
ApoI 972 AAATTT [GA]^AATT[CT]
AspHI 523 GAGCTC G[AT]GC[AT]^C
AvaI 1122 CTCGGG C^[CT]CG[GA]G
BbeI 1277 GGCGCC GGCGC^C
BcgI 1045 GCAAAGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 102 GGATAC GTATCC(6/5)
BclI 281 TGATCA T^GATCA
BfrI 295 CTTAAG C^TTAAG
BlpI 1017 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1017 GCTGAGC GC^T[ACGT]AGC
BsaI 1050 GGTCTC GGTCTC(1/5)
BsaWI 1346 ACCGGT [AT]^CCGG[AT]
BsgI 1180 GTGCAG GTGCAG(16/14)
BsiHKAI 523 GAGCTC G[AT]GC[AT]^C
BsoBI 1122 CTCGGG C^[CT]CG[GA]G
Bsp1407I 121 TGTACA T^GTACA
BspLU11I 1360 ACATGT A^CATGT
BsrDI 459 GCAATG GCAATG(2/0)
BsrGI 121 TGTACA T^GTACA
BstAPI 1104 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 795 GGTTACC G^GT[ACGT]ACC
BstPI 795 GGTTACC G^GT[ACGT]ACC
BstUI 235 CGCG CG^CG
BstXI 528 CCAGGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1087 CCTGAGG CC^T[ACGT]AGG
CelII 1017 GCTGAGC GC^T[ACGT]AGC
EarI 138 GAAGAG CTCTTC(1/4)
Eco81I 1087 CCTGAGG CC^T[ACGT]AGG
EcoO65I 795 GGTTACC G^GT[ACGT]ACC
FbaI 281 TGATCA T^GATCA
HgaI 1211 GCGTC GACGC(5/10)
KasI 1277 GGCGCC G^GCGCC
Ksp632I 138 GAAGAG CTCTTC(1/4)
MfeI 355 CAATTG C^AATTG
MspA1I 517 CAGCTG C[AC]G^C[GT]G
MunI 355 CAATTG C^AATTG
MvnI 235 CGCG CG^CG
NarI 1277 GGCGCC GG^CGCC
NheI 603 GCTAGC G^CTAGC
PflMI 743 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1346 ACCGGT A^CCGGT
PvuII 517 CAGCTG CAG^CTG
RsaI 122 GTAC GT^AC
SacI 523 GAGCTC GAGCT^C
SapI 138 GAAGAGC GCTCTTC(1/4)
SexAI 938 ACCTGGT A^CC[AT]GGT
SfoI 1277 GGCGCC GGC^GCC
SspBI 121 TGTACA T^GTACA
TaqI 1054 TCGA T^CGA
Tsp45I 1118 GTGAC ^GT[GC]AC
TthHB8I 1054 TCGA T^CGA
Van91I 743 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 1210 GGCGTC G[GA]^CG[CT]C
1277 GGCGCC
BcnI 6 CCGGG CC^[GC]GG
1176 CCCGG
BsaHI 1210 GGCGTC G[GA]^CG[CT]C
1277 GGCGCC
BspMI 395 ACCTGC ACCTGC(4/8)
1330 ACCTGC
BsrBI 835 CCGCTC CCGCTC(-3/-3)
1288 GAGCGG
EcoNI 396 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
849 CCTGCCCCAGG
HaeII 48 GGCGCT [GA]GCGC^[CT]
1277 GGCGCC
Hin1I 1210 GGCGTC G[GA]^CG[CT]C
1277 GGCGCC
MaeIII 796 GTTAC ^GT[ACGT]AC
1118 GTGAC
MseI 205 TTAA T^TAA
296 TTAA
MslI 594 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1173 CACCCCGGTG
NaeI 1216 GCCGGC GCC^GGC
1265 GCCGGC
NciI 6 CCGGG CC^[GC]GG
1176 CCCGG
NgoMIV 1216 GCCGGC G^CCGGC
1265 GCCGGC
NspI 547 ACATGC [GA]CATG^[CT]
1360 ACATGT
SmlI 243 CTCAAG C^T[CT][GA]AG
295 CTTAAG
StuI 617 AGGCCT AGG^CCT
857 AGGCCT
Tru9I 205 TTAA T^TAA
296 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI TseI Tsp509I
TspRI