Restriction map for
pF1KB7508
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvrII BamHI BbrPI BbsI
BbvCI BcgI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI HincII
HindII HindIII HpaI HphI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MroI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoII
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 115 GGTACC G^GTACC
AccII 301 CGCG CG^CG
AcyI 189 GGCGCC G[GA]^CG[CT]C
AfaI 116 GTAC GT^AC
AflIII 180 ACATGT A^C[GA][CT]GT
AlwI 453 GGATC GGATC(4/5)
Asp718I 115 GGTACC G^GTACC
AviII 539 TGCGCA TGC^GCA
BanII 586 GGGCTC G[GA]GC[CT]^C
BbeI 189 GGCGCC GGCGC^C
BciVI 99 GTATCC GTATCC(6/5)
BglI 334 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 586 GGGCTC G[AGT]GC[ACT]^C
BsaHI 189 GGCGCC G[GA]^CG[CT]C
BsgI 442 CTGCAC GTGCAG(16/14)
BsmFI 123 GTCCC GGGAC(10/14)
Bsp1286I 586 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 180 ACATGT A^CATGT
BsrBI 424 GAGCGG CCGCTC(-3/-3)
BssSI 589 CTCGTG CACGAG(-5/-1)
BstF5I 45 GGATG GGATG(2/0)
BstUI 301 CGCG CG^CG
DdeI 602 CTAAG C^T[ACGT]AG
DraII 434 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 434 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 386 CCAAGG C^C[AT][AT]GG
FauI 154 GCGGG CCCGC(4/6)
FokI 45 GGATG GGATG(9/13)
FspI 539 TGCGCA TGC^GCA
Hin1I 189 GGCGCC G[GA]^CG[CT]C
KasI 189 GGCGCC G^GCGCC
KpnI 115 GGTACC GGTAC^C
MaeIII 58 GTTAC ^GT[ACGT]AC
MspA1I 168 CAGCGG C[AC]G^C[GT]G
MvnI 301 CGCG CG^CG
NarI 189 GGCGCC GG^CGCC
NlaIII 181 CATG CATG^
NspI 180 ACATGT [GA]CATG^[CT]
PaeR7I 636 CTCGAG C^TCGAG
PpuMI 434 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 116 GTAC GT^AC
SfoI 189 GGCGCC GGC^GCC
SmaI 195 CCCGGG CCC^GGG
StuI 332 AGGCCT AGG^CCT
StyI 386 CCAAGG C^C[AT][AT]GG
XhoI 636 CTCGAG C^TCGAG
XmaCI 195 CCCGGG C^CCGGG
XmaI 195 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaI 195 CCCGGG C^[CT]CG[GA]G
636 CTCGAG
BalI 362 TGGCCA TGG^CCA
559 TGGCCA
BanI 115 GGTACC G^G[CT][GA]CC
189 GGCGCC
BseRI 309 GAGGAG GAGGAG(10/8)
611 GAGGAG
BsoBI 195 CCCGGG C^[CT]CG[GA]G
636 CTCGAG
BsrFI 278 ACCGGC [GA]^CCGG[CT]
446 ACCGGC
BstAPI 245 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
432 GCAGGACCTGC
Cac8I 200 GCCTGC GC[ACGT]^[ACGT]GC
241 GCTTGC
Cfr10I 278 ACCGGC [GA]^CCGG[CT]
446 ACCGGC
HaeII 189 GGCGCC [GA]GCGC^[CT]
406 AGCGCC
HinfI 396 GATTC G^A[ACGT]TC
625 GATTC
MluNI 362 TGGCCA TGG^CCA
559 TGGCCA
MscI 362 TGGCCA TGG^CCA
559 TGGCCA
SfaNI 468 GATGC GCATC(5/9)
650 GATGC
SmlI 520 CTTGAG C^T[CT][GA]AG
636 CTCGAG
TfiI 396 GATTC G^A[AT]TC
625 GATTC
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BsaJI BsiYI BslI BspMI
BsrI BssKI BstNI CfoI Cfr13I
DpnII EaeI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIV PstI
Sau3AI Sau96I ScrFI SfcI TaqI
TseI TspRI TthHB8I