Restriction map for
pF1KB6195
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI AspEI AviII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BsaAI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI TaqI
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 117 GGTACC G^GTACC
AccII 282 CGCG CG^CG
AccIII 533 TCCGGA T^CCGGA
AcsI 1055 AAATTC [GA]^AATT[CT]
AcyI 1497 GGCGCC G[GA]^CG[CT]C
ApoI 1055 AAATTC [GA]^AATT[CT]
Asp700I 352 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 117 GGTACC G^GTACC
AspI 1791 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 147 CTCGGG C^[CT]CG[GA]G
BalI 833 TGGCCA TGG^CCA
BbeI 1497 GGCGCC GGCGC^C
BlpI 1879 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1879 GCTGAGC GC^T[ACGT]AGC
BsaHI 1497 GGCGCC G[GA]^CG[CT]C
BsaWI 533 TCCGGA [AT]^CCGG[AT]
BseAI 533 TCCGGA T^CCGGA
BsgI 374 CTGCAC GTGCAG(16/14)
BsmBI 1143 CGTCTC CGTCTC(1/5)
BsmI 465 GCATTC GAATGC(1/-1)
BsoBI 147 CTCGGG C^[CT]CG[GA]G
BspEI 533 TCCGGA T^CCGGA
BspMI 114 GCAGGT ACCTGC(4/8)
BsrDI 1560 CATTGC GCAATG(2/0)
BstUI 282 CGCG CG^CG
BstXI 881 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1879 GCTGAGC GC^T[ACGT]AGC
CfoI 1498 GCGC GCG^C
DrdI 886 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 833 TGGCCA [CT]^GGCC[GA]
EcoNI 1568 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1497 GGCGCC [GA]GCGC^[CT]
HgaI 207 GACGC GACGC(5/10)
HhaI 1498 GCGC GCG^C
Hin1I 1497 GGCGCC G[GA]^CG[CT]C
HinP1I 1498 GCGC G^CGC
HincII 276 GTCAAC GT[CT]^[GA]AC
HindII 276 GTCAAC GT[CT]^[GA]AC
KasI 1497 GGCGCC G^GCGCC
KpnI 117 GGTACC GGTAC^C
KspI 281 CCGCGG CCGC^GG
MaeII 167 ACGT A^CGT
MluNI 833 TGGCCA TGG^CCA
MroI 533 TCCGGA T^CCGGA
MscI 833 TGGCCA TGG^CCA
MslI 1965 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 282 CGCG CG^CG
NarI 1497 GGCGCC GG^CGCC
NcoI 1664 CCATGG C^CATGG
NspI 1062 GCATGT [GA]CATG^[CT]
PflFI 1791 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1255 CTGCAG CTGCA^G
SacII 281 CCGCGG CCGC^GG
ScaI 1303 AGTACT AGT^ACT
SexAI 887 ACCTGGT A^CC[AT]GGT
SfoI 1497 GGCGCC GGC^GCC
SmlI 1843 CTTGAG C^T[CT][GA]AG
SpeI 448 ACTAGT A^CTAGT
Tth111I 1791 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 352 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
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ApaI 1531 GGGCCC GGGCC^C
1658 GGGCCC
AspHI 1702 GAGCTC G[AT]GC[AT]^C
1846 GAGCTC
AvrII 1215 CCTAGG C^CTAGG
1600 CCTAGG
BanI 117 GGTACC G^G[CT][GA]CC
1497 GGCGCC
BcnI 980 CCCGG CC^[GC]GG
1494 CCCGG
BglII 335 AGATCT A^GATCT
1009 AGATCT
BlnI 1215 CCTAGG C^CTAGG
1600 CCTAGG
BsiHKAI 1702 GAGCTC G[AT]GC[AT]^C
1846 GAGCTC
EcoRV 64 GATATC GAT^ATC
316 GATATC
FauI 439 GCGGG CCCGC(4/6)
798 GCGGG
MseI 869 TTAA T^TAA
1967 TTAA
NciI 980 CCCGG CC^[GC]GG
1494 CCCGG
SacI 1702 GAGCTC GAGCT^C
1846 GAGCTC
SapI 649 GCTCTTC GCTCTTC(1/4)
1034 GCTCTTC
SfiI 945 GGCCTCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
954 GGCCTCTGTGGCC
TfiI 530 GAATC G^A[AT]TC
1443 GATTC
Tru9I 869 TTAA T^TAA
1967 TTAA
Tsp509I 1056 AATT ^AATT
1094 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AvaII BanII BbsI BbvI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TseI
Tsp45I TspRI Van91I XcmI XhoII