Restriction map for
pF1KB6974
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# NO CUT ENZYMES ( 180 enzymes) #
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Acc65I AccI AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MflI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TfiI Tth111I TthHB8I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 111 GACGTC GACGT^C
AcsI 447 GAATTT [GA]^AATT[CT]
AcyI 111 GACGTC G[GA]^CG[CT]C
AfaI 428 GTAC GT^AC
AlwI 685 GATCC GGATC(4/5)
ApoI 447 GAATTT [GA]^AATT[CT]
BbvI 21 GCAGC GCAGC(8/12)
BsaBI 568 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 111 GACGTC G[GA]^CG[CT]C
BseRI 16 GAGGAG GAGGAG(10/8)
BsmAI 238 GTCTC GTCTC(1/5)
CfoI 24 GCGC GCG^C
Cfr13I 39 GGCCC G^G[ACGT]CC
DraI 249 TTTAAA TTT^AAA
DraII 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 423 CCATGG C^C[GA][CT]GG
EarI 362 GAAGAG CTCTTC(1/4)
EcoO109I 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 589 GATATC GAT^ATC
EcoT14I 423 CCATGG C^C[AT][AT]GG
Fnu4HI 21 GCAGC GC^[ACGT]GC
HaeIII 39 GGCC GG^CC
HhaI 24 GCGC GCG^C
Hin1I 111 GACGTC G[GA]^CG[CT]C
HinP1I 24 GCGC G^CGC
ItaI 21 GCAGC GC^[ACGT]GC
Ksp632I 362 GAAGAG CTCTTC(1/4)
MamI 568 GATCATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 440 CAATTG C^AATTG
MslI 102 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 440 CAATTG C^AATTG
NcoI 423 CCATGG C^CATGG
NlaIII 424 CATG CATG^
RsaI 428 GTAC GT^AC
Sau96I 39 GGCCC G^G[ACGT]CC
SmlI 154 CTCAAG C^T[CT][GA]AG
SspI 204 AATATT AAT^ATT
StyI 423 CCATGG C^C[AT][AT]GG
TseI 21 GCAGC G^C[AT]GC
TspRI 168 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 222 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BsiYI 594 CCAGTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
645 CCAAGAAATGG
BslI 594 CCAGTTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
645 CCAAGAAATGG
BsrI 433 CCAGT ACTGG(1/-1)
594 CCAGT
BssKI 42 CCTGG ^CC[ACGT]GG
213 CCAGG
BstNI 42 CCTGG CC^[AT]GG
213 CCAGG
EcoRII 42 CCTGG ^CC[AT]GG
213 CCAGG
HphI 291 GGTGA GGTGA(8/7)
98 TCACC
MaeII 112 ACGT A^CGT
620 ACGT
MspA1I 314 CAGCTG C[AC]G^C[GT]G
722 CAGCTG
MvaI 42 CCTGG CC^[AT]GG
213 CCAGG
PflMI 594 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCAAGAAATGG
PvuII 314 CAGCTG CAG^CTG
722 CAGCTG
ScrFI 42 CCTGG CC^[ACGT]GG
213 CCAGG
SfaNI 475 GATGC GCATC(5/9)
607 GATGC
Van91I 594 CCAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
645 CCAAGAAATGG
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BfaI BpmI BsaJI BstF5I
DdeI DpnII FokI HinfI MaeI
MaeIII MboI MboII MnlI MseI
MwoI NdeII PleI Sau3AI Tru9I
Tsp45I Tsp509I