Restriction map for
pF1KB6337
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MfeI MflI
MluI MluNI MroI MscI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 331 CGCG CG^CG
AciI 701 GCGG CCGC(-3/-1)
BanII 242 GGGCTC G[GA]GC[CT]^C
BbvI 199 GCAGC GCAGC(8/12)
BmyI 242 GGGCTC G[AGT]GC[ACT]^C
BsaBI 541 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 704 GAGACC GGTCTC(1/5)
BseRI 107 CTCCTC GAGGAG(10/8)
BsmFI 225 GTCCC GGGAC(10/14)
Bsp1286I 242 GGGCTC G[AGT]GC[ACT]^C
BstEII 337 GGTGACC G^GT[ACGT]ACC
BstPI 337 GGTGACC G^GT[ACGT]ACC
BstUI 331 CGCG CG^CG
DpnII 541 GATC ^GATC
DraII 223 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 523 GACAGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 223 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 337 GGTGACC G^GT[ACGT]ACC
Fnu4HI 199 GCAGC GC^[ACGT]GC
HincII 192 GTTGAC GT[CT]^[GA]AC
HindII 192 GTTGAC GT[CT]^[GA]AC
ItaI 199 GCAGC GC^[ACGT]GC
MamI 541 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 541 GATC ^GATC
MslI 469 CACAATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 355 CAGCTG C[AC]G^C[GT]G
MvnI 331 CGCG CG^CG
MwoI 169 GCTTTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 541 GATC ^GATC
PpuMI 223 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 197 CTGCAG CTGCA^G
PvuII 355 CAGCTG CAG^CTG
SapI 262 GAAGAGC GCTCTTC(1/4)
Sau3AI 541 GATC ^GATC
TaqI 543 TCGA T^CGA
TfiI 631 GATTC G^A[AT]TC
TseI 199 GCAGC G^C[AT]GC
TspRI 205 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 543 TCGA T^CGA
XcmI 228 CCAGGTCATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 201 AGCT AG^CT
356 AGCT
BsiYI 406 CCTAAAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
565 CCTGTGATTGG
BslI 406 CCTAAAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
565 CCTGTGATTGG
BsmAI 614 GAGAC GTCTC(1/5)
704 GAGAC
BsrDI 536 GCAATG GCAATG(2/0)
623 GCAATG
BsrI 185 ACTGG ACTGG(1/-1)
755 ACTGG
BssSI 618 CTCGTG CACGAG(-5/-1)
710 CTCGTG
BstF5I 26 CATCC GGATG(2/0)
417 GGATG
EarI 262 GAAGAG CTCTTC(1/4)
611 GAAGAG
FokI 26 CATCC GGATG(9/13)
417 GGATG
HaeIII 685 GGCC GG^CC
716 GGCC
HphI 337 GGTGA GGTGA(8/7)
512 TCACC
Ksp632I 262 GAAGAG CTCTTC(1/4)
611 GAAGAG
NlaIV 224 GGTCCC GG[ACGT]^[ACGT]CC
433 GGAACC
PleI 465 GACTC GAGTC(4/5)
616 GACTC
SfaNI 25 GCATC GCATC(5/9)
478 GCATC
SfcI 12 CTATAG C^T[GA][CT]AG
197 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AvaII BsaJI BssKI BstNI Cfr13I
DdeI EcoRII HinfI MaeIII MboII
MnlI MseI MvaI NlaIII Sau96I
ScrFI Tru9I Tsp45I Tsp509I