Restriction map for
pF1KB6936
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglI BlnI BpuAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 116 GGTACC G^GTACC
AccII 524 CGCG CG^CG
AcyI 246 GACGCC G[GA]^CG[CT]C
AocI 567 CCTCAGG CC^T[ACGT]AGG
Asp718I 116 GGTACC G^GTACC
BamHI 497 GGATCC G^GATCC
BanI 116 GGTACC G^G[CT][GA]CC
BcgI 344 GCAGCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 476 AGATCT A^GATCT
BlpI 421 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 421 GCTGAGC GC^T[ACGT]AGC
BsaHI 246 GACGCC G[GA]^CG[CT]C
BsiEI 122 CGATCG CG[GA][CT]^CG
BsmAI 653 GTCTC GTCTC(1/5)
BssHII 523 GCGCGC G^CGCGC
BstUI 524 CGCG CG^CG
Bsu36I 567 CCTCAGG CC^T[ACGT]AGG
CelII 421 GCTGAGC GC^T[ACGT]AGC
DraIII 532 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 246 GACGCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 567 CCTCAGG CC^T[ACGT]AGG
EcoNI 391 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 293 CCATGG C^C[AT][AT]GG
HaeII 425 AGCGCC [GA]GCGC^[CT]
Hin1I 246 GACGCC G[GA]^CG[CT]C
HinfI 651 GAGTC G^A[ACGT]TC
HphI 317 TCACC GGTGA(8/7)
KpnI 116 GGTACC GGTAC^C
MboII 479 TCTTC GAAGA(8/7)
MvnI 524 CGCG CG^CG
NcoI 293 CCATGG C^CATGG
PflMI 531 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 651 GAGTC GAGTC(4/5)
PshAI 646 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 122 CGATCG CGAT^CG
ScaI 491 AGTACT AGT^ACT
StyI 293 CCATGG C^C[AT][AT]GG
Van91I 531 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 668 CCACAATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 498 GATCC GGATC(4/5)
497 GGATC
ApaI 67 GGGCCC GGGCC^C
541 GGGCCC
AspHI 307 GTGCTC G[AT]GC[AT]^C
370 GAGCAC
BsaAI 192 CACGTA [CT]AC^GT[GA]
304 TACGTG
BsgI 264 CTGCAC GTGCAG(16/14)
439 GTGCAG
BsiHKAI 307 GTGCTC G[AT]GC[AT]^C
370 GAGCAC
BsrDI 186 CATTGC GCAATG(2/0)
99 GCAATG
BsrI 494 ACTGG ACTGG(1/-1)
647 ACTGG
BstF5I 564 CATCC GGATG(2/0)
276 GGATG
BstYI 476 AGATCT [GA]^GATC[CT]
497 GGATCC
DsaI 128 CCGTGG C^C[GA][CT]GG
293 CCATGG
FokI 564 CATCC GGATG(9/13)
276 GGATG
HgaI 246 GACGC GACGC(5/10)
510 GCGTC
MflI 476 AGATCT [GA]^GATC[CT]
497 GGATCC
NlaIII 207 CATG CATG^
294 CATG
PpuMI 457 GGGTCCT [GA]G^G[AT]CC[CT]
617 AGGACCT
PstI 51 CTGCAG CTGCA^G
238 CTGCAG
Tsp45I 316 GTCAC ^GT[GC]AC
644 GTGAC
XhoII 476 AGATCT [GA]^GATC[CT]
497 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsoBI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmaI TseI TspRI XmaCI XmaI