Restriction map for
pF1KB4011
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AflIII 606 ACACGT A^C[GA][CT]GT
Aor51HI 1103 AGCGCT AGC^GCT
ApaI 31 GGGCCC GGGCC^C
AvaI 412 CCCGGG C^[CT]CG[GA]G
AviII 1080 TGCGCA TGC^GCA
BalI 317 TGGCCA TGG^CCA
BbeI 1335 GGCGCC GGCGC^C
BbrPI 607 CACGTG CAC^GTG
BcgI 1007 GCATGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 658 CTAG C^TAG
BglI 1069 GCCGTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 388 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 388 GCTGAGC GC^T[ACGT]AGC
BsaAI 607 CACGTG [CT]AC^GT[GA]
BsaI 10 GAGACC GGTCTC(1/5)
BsgI 1417 GTGCAG GTGCAG(16/14)
BsmAI 10 GAGAC GTCTC(1/5)
BsoBI 412 CCCGGG C^[CT]CG[GA]G
BspMI 149 GCAGGT ACCTGC(4/8)
BsrDI 789 CATTGC GCAATG(2/0)
BsrFI 170 GCCGGC [GA]^CCGG[CT]
BssSI 1537 CACGAG CACGAG(-5/-1)
BstAPI 1083 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1599 GGTCACC G^GT[ACGT]ACC
BstPI 1599 GGTCACC G^GT[ACGT]ACC
BstXI 935 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 388 GCTGAGC GC^T[ACGT]AGC
Cfr10I 170 GCCGGC [GA]^CCGG[CT]
Eco47III 1103 AGCGCT AGC^GCT
EcoNI 81 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1599 GGTCACC G^GT[ACGT]ACC
EcoT14I 113 CCAAGG C^C[AT][AT]GG
FseI 169 GGCCGGCC GGCCGG^CC
FspI 1080 TGCGCA TGC^GCA
KasI 1335 GGCGCC G^GCGCC
MaeI 658 CTAG C^TAG
MluNI 317 TGGCCA TGG^CCA
MscI 317 TGGCCA TGG^CCA
NaeI 170 GCCGGC GCC^GGC
NarI 1335 GGCGCC GG^CGCC
NgoMIV 170 GCCGGC G^CCGGC
PmaCI 607 CACGTG CAC^GTG
PmlI 607 CACGTG CAC^GTG
PvuII 163 CAGCTG CAG^CTG
SfoI 1335 GGCGCC GGC^GCC
SmaI 412 CCCGGG CCC^GGG
SphI 1007 GCATGC GCATG^C
StyI 113 CCAAGG C^C[AT][AT]GG
TfiI 272 GAATC G^A[AT]TC
Tsp509I 646 AATT ^AATT
XcmI 677 CCAGCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 412 CCCGGG C^CCGGG
XmaI 412 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 570 GACGCC G[GA]^CG[CT]C
1335 GGCGCC
AfaI 666 GTAC GT^AC
1551 GTAC
Alw44I 153 GTGCAC G^TGCAC
1534 GTGCAC
AlwNI 1084 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1243 CAGGAGCTG
ApaLI 153 GTGCAC G^TGCAC
1534 GTGCAC
Asp700I 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1320 GAAGGGCTTC
BamHI 128 GGATCC G^GATCC
840 GGATCC
BpmI 82 CTGGAG CTGGAG(16/14)
1507 CTGGAG
BsaHI 570 GACGCC G[GA]^CG[CT]C
1335 GGCGCC
BsrBI 51 CCGCTC CCGCTC(-3/-3)
1639 GAGCGG
DsaI 935 CCACGG C^C[GA][CT]GG
1340 CCGTGG
HaeII 1103 AGCGCT [GA]GCGC^[CT]
1335 GGCGCC
Hin1I 570 GACGCC G[GA]^CG[CT]C
1335 GGCGCC
MaeII 536 ACGT A^CGT
608 ACGT
PpuMI 91 GGGTCCC [GA]G^G[AT]CC[CT]
1199 AGGACCT
RsaI 666 GTAC GT^AC
1551 GTAC
SacI 310 GAGCTC GAGCT^C
519 GAGCTC
XmnI 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1320 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I XhoII