Restriction map for
pF1KE2390
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SapI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tru9I
XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 83 GTCGAC GT^[AC][GT]AC
AcsI 363 GAATTT [GA]^AATT[CT]
AlwI 667 GATCC GGATC(4/5)
AlwNI 444 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 363 GAATTT [GA]^AATT[CT]
Asp700I 96 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 148 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 670 CCTAGG C^CTAGG
BanI 42 GGCGCC G^G[CT][GA]CC
BbeI 42 GGCGCC GGCGC^C
BbsI 796 GTCTTC GAAGAC(2/6)
BglI 639 GCCCTCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1043 AGATCT A^GATCT
BlnI 670 CCTAGG C^CTAGG
BlpI 261 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 261 GCTGAGC GC^T[ACGT]AGC
BpuAI 796 GTCTTC GAAGAC(2/6)
BsaAI 1470 CACGTA [CT]AC^GT[GA]
BseRI 1311 CTCCTC GAGGAG(10/8)
BsgI 994 CTGCAC GTGCAG(16/14)
BsmBI 1308 CGTCTC CGTCTC(1/5)
BspHI 591 TCATGA T^CATGA
BsrDI 756 CATTGC GCAATG(2/0)
BstAPI 443 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 462 GGTCACC G^GT[ACGT]ACC
BstPI 462 GGTCACC G^GT[ACGT]ACC
BstXI 1391 CCATGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1043 AGATCT [GA]^GATC[CT]
CelII 261 GCTGAGC GC^T[ACGT]AGC
DrdI 382 GACATTAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 670 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 462 GGTCACC G^GT[ACGT]ACC
EcoT22I 765 ATGCAT ATGCA^T
FauI 135 GCGGG CCCGC(4/6)
KasI 42 GGCGCC G^GCGCC
KspI 63 CCGCGG CCGC^GG
MflI 1043 AGATCT [GA]^GATC[CT]
NarI 42 GGCGCC GG^CGCC
NsiI 765 ATGCAT ATGCA^T
PflFI 148 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1135 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 86 GACTC GAGTC(4/5)
PshAI 1446 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 591 TCATGA T^CATGA
SacII 63 CCGCGG CCGC^GG
SalI 83 GTCGAC G^TCGAC
SexAI 743 ACCTGGT A^CC[AT]GGT
SfoI 42 GGCGCC GGC^GCC
SmaI 429 CCCGGG CCC^GGG
SspI 1166 AATATT AAT^ATT
StuI 549 AGGCCT AGG^CCT
Tth111I 148 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1135 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1135 CCAGGAAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1043 AGATCT [GA]^GATC[CT]
XmaCI 429 CCCGGG C^CCGGG
XmaI 429 CCCGGG C^CCGGG
XmnI 96 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 64 CGCG CG^CG
134 CGCG
AccIII 1175 TCCGGA T^CCGGA
1290 TCCGGA
AspHI 219 GAGCAC G[AT]GC[AT]^C
280 GAGCAC
AvaI 429 CCCGGG C^[CT]CG[GA]G
1093 CCCGAG
BfaI 671 CTAG C^TAG
1450 CTAG
BsaWI 1175 TCCGGA [AT]^CCGG[AT]
1290 TCCGGA
BseAI 1175 TCCGGA T^CCGGA
1290 TCCGGA
BsiHKAI 219 GAGCAC G[AT]GC[AT]^C
280 GAGCAC
BsoBI 429 CCCGGG C^[CT]CG[GA]G
1093 CCCGAG
BspDI 292 ATCGAT AT^CGAT
1258 ATCGAT
BspEI 1175 TCCGGA T^CCGGA
1290 TCCGGA
BsrBI 46 CCGCTC CCGCTC(-3/-3)
1002 CCGCTC
BstUI 64 CGCG CG^CG
134 CGCG
ClaI 292 ATCGAT AT^CGAT
1258 ATCGAT
HincII 83 GTCGAC GT[CT]^[GA]AC
748 GTCAAC
HindII 83 GTCGAC GT[CT]^[GA]AC
748 GTCAAC
MaeI 671 CTAG C^TAG
1450 CTAG
MaeIII 463 GTCAC ^GT[ACGT]AC
955 GTCAC
MroI 1175 TCCGGA T^CCGGA
1290 TCCGGA
MvnI 64 CGCG CG^CG
134 CGCG
NcoI 944 CCATGG C^CATGG
1391 CCATGG
PvuII 447 CAGCTG CAG^CTG
1336 CAGCTG
TfiI 295 GATTC G^A[AT]TC
316 GAATC
Tsp45I 463 GTCAC ^GT[GC]AC
955 GTCAC
Tsp509I 364 AATT ^AATT
490 AATT
TspRI 619 CAGTG CA[GC]TG[ACGT][ACGT]^
1123 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TthHB8I