Restriction map for
pF1KB6604
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmFI BsmI BspDI
BspLU11I BspMI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MaeI MamI
MfeI MluI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PleI PmeI PpuMI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Alw44I 109 GTGCAC G^TGCAC
AlwNI 1138 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 109 GTGCAC G^TGCAC
AspI 391 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 114 CTCGAG C^[CT]CG[GA]G
BbrPI 4 CACGTG CAC^GTG
BbsI 328 GTCTTC GAAGAC(2/6)
BglI 946 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 328 GTCTTC GAAGAC(2/6)
BsaAI 4 CACGTG [CT]AC^GT[GA]
BsaI 1019 GAGACC GGTCTC(1/5)
BsgI 1135 GTGCAG GTGCAG(16/14)
BsoBI 114 CTCGAG C^[CT]CG[GA]G
Bsp1407I 257 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BsrFI 128 ACCGGC [GA]^CCGG[CT]
BsrGI 257 TGTACA T^GTACA
BstXI 266 CCATGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 128 ACCGGC [GA]^CCGG[CT]
DraIII 700 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 622 CTCTTC CTCTTC(1/4)
EcoT14I 266 CCATGG C^C[AT][AT]GG
HincII 409 GTCAAC GT[CT]^[GA]AC
HindII 409 GTCAAC GT[CT]^[GA]AC
HinfI 613 GAATC G^A[ACGT]TC
HphI 380 TCACC GGTGA(8/7)
Ksp632I 622 CTCTTC CTCTTC(1/4)
KspI 715 CCGCGG CCGC^GG
MaeIII 505 GTCAC ^GT[ACGT]AC
MspA1I 715 CCGCGG C[AC]G^C[GT]G
NcoI 266 CCATGG C^CATGG
PaeR7I 114 CTCGAG C^TCGAG
PflFI 391 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 699 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4 CACGTG CAC^GTG
PmlI 4 CACGTG CAC^GTG
PshAI 1140 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 733 CTGCAG CTGCA^G
RcaI 398 TCATGA T^CATGA
SacII 715 CCGCGG CCGC^GG
SfcI 733 CTGCAG C^T[GA][CT]AG
SgrAI 127 CACCGGCG C[GA]^CCGG[CT]G
SmlI 114 CTCGAG C^T[CT][GA]AG
SspBI 257 TGTACA T^GTACA
StuI 983 AGGCCT AGG^CCT
StyI 266 CCATGG C^C[AT][AT]GG
TfiI 613 GAATC G^A[AT]TC
Tsp45I 505 GTCAC ^GT[GC]AC
Tth111I 391 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 699 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 239 CCATGTTCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 114 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 521 TCCGGA T^CCGGA
569 TCCGGA
AcsI 433 GAATTC [GA]^AATT[CT]
517 GAATTC
ApoI 433 GAATTC [GA]^AATT[CT]
517 GAATTC
BalI 353 TGGCCA TGG^CCA
944 TGGCCA
BbeI 27 GGCGCC GGCGC^C
131 GGCGCC
BpmI 214 CTCCAG CTGGAG(16/14)
592 CTCCAG
BseAI 521 TCCGGA T^CCGGA
569 TCCGGA
BseRI 277 GAGGAG GAGGAG(10/8)
658 GAGGAG
BsmBI 562 GAGACG CGTCTC(1/5)
748 GAGACG
BspEI 521 TCCGGA T^CCGGA
569 TCCGGA
BsrBI 906 CCGCTC CCGCTC(-3/-3)
148 GAGCGG
BsrI 701 ACTGG ACTGG(1/-1)
1037 ACTGG
BssSI 997 CACGAG CACGAG(-5/-1)
415 CTCGTG
EcoRI 433 GAATTC G^AATTC
517 GAATTC
KasI 27 GGCGCC G^GCGCC
131 GGCGCC
MluNI 353 TGGCCA TGG^CCA
944 TGGCCA
MroI 521 TCCGGA T^CCGGA
569 TCCGGA
MscI 353 TGGCCA TGG^CCA
944 TGGCCA
NarI 27 GGCGCC GG^CGCC
131 GGCGCC
SfoI 27 GGCGCC GGC^GCC
131 GGCGCC
Tsp509I 434 AATT ^AATT
518 AATT
TspRI 14 CACTG CA[GC]TG[ACGT][ACGT]^
700 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaII BanI
BanII BbvI BcnI BmyI BsaHI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI MaeII MboI
MboII MflI MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TthHB8I
XhoII