Restriction map for
pF1KB6616
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AocI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 926 TCCGGA T^CCGGA
AcsI 293 AAATTC [GA]^AATT[CT]
AflIII 68 ACACGT A^C[GA][CT]GT
AhdI 709 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 517 GTGCAC G^TGCAC
AlwNI 19 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1226 GGGCCC GGGCC^C
ApaLI 517 GTGCAC G^TGCAC
ApoI 293 AAATTC [GA]^AATT[CT]
Asp700I 435 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 709 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 260 GGATCC G^GATCC
BglI 1240 GCCCGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1197 GCTGAGC GC^T[ACGT]AGC
BpmI 490 CTGGAG CTGGAG(16/14)
Bpu1102I 1197 GCTGAGC GC^T[ACGT]AGC
BseAI 926 TCCGGA T^CCGGA
BsmAI 208 GAGAC GTCTC(1/5)
BsmBI 208 GAGACG CGTCTC(1/5)
BspEI 926 TCCGGA T^CCGGA
BsrBI 841 CCGCTC CCGCTC(-3/-3)
BstBI 763 TTCGAA TT^CGAA
BstEII 726 GGTCACC G^GT[ACGT]ACC
BstPI 726 GGTCACC G^GT[ACGT]ACC
BstXI 674 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1197 GCTGAGC GC^T[ACGT]AGC
Eam1105I 709 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1155 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 726 GGTCACC G^GT[ACGT]ACC
HaeII 1145 AGCGCC [GA]GCGC^[CT]
HgaI 210 GACGC GACGC(5/10)
MroI 926 TCCGGA T^CCGGA
NaeI 1274 GCCGGC GCC^GGC
NgoMIV 1274 GCCGGC G^CCGGC
NspV 763 TTCGAA TT^CGAA
PflMI 333 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 389 AGTACT AGT^ACT
SfuI 763 TTCGAA TT^CGAA
SgrAI 555 CACCGGTG C[GA]^CCGG[CT]G
SmaI 822 CCCGGG CCC^GGG
SphI 404 GCATGC GCATG^C
StuI 1247 AGGCCT AGG^CCT
Tsp509I 294 AATT ^AATT
Van91I 333 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 822 CCCGGG C^CCGGG
XmaI 822 CCCGGG C^CCGGG
XmnI 435 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AgeI 556 ACCGGT A^CCGGT
989 ACCGGT
AspHI 517 GTGCAC G[AT]GC[AT]^C
1200 GAGCAC
AvaI 664 CCCGAG C^[CT]CG[GA]G
822 CCCGGG
BseRI 306 GAGGAG GAGGAG(10/8)
1123 GAGGAG
BsiHKAI 517 GTGCAC G[AT]GC[AT]^C
1200 GAGCAC
BsoBI 664 CCCGAG C^[CT]CG[GA]G
822 CCCGGG
BstAPI 408 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1244 GCAAGGCCTGC
DsaI 459 CCACGG C^C[GA][CT]GG
746 CCACGG
EaeI 590 TGGCCG [CT]^GGCC[GA]
881 CGGCCA
EcoT14I 983 CCAAGG C^C[AT][AT]GG
1160 CCAAGG
HinfI 88 GACTC G^A[ACGT]TC
202 GACTC
MaeII 70 ACGT A^CGT
281 ACGT
MslI 319 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1002 CATGACTGTG
NspI 29 GCATGT [GA]CATG^[CT]
404 GCATGC
PinAI 556 ACCGGT A^CCGGT
989 ACCGGT
PleI 88 GACTC GAGTC(4/5)
202 GACTC
SfaNI 864 GATGC GCATC(5/9)
83 GCATC
SmlI 238 CTCAAG C^T[CT][GA]AG
868 CTTGAG
StyI 983 CCAAGG C^C[AT][AT]GG
1160 CCAAGG
Tsp45I 727 GTCAC ^GT[GC]AC
1133 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsaWI BsgI BsiYI
BslI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI TaqI TseI TspRI
TthHB8I XhoII