Restriction map for
pF1KB6664
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AviII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BlpI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AclI 184 AACGTT AA^CGTT
AcyI 1242 GACGCC G[GA]^CG[CT]C
AflII 163 CTTAAG C^TTAAG
AseI 127 ATTAAT AT^TAAT
AsnI 127 ATTAAT AT^TAAT
AspHI 1119 GAGCTC G[AT]GC[AT]^C
AspI 1279 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 27 CCCGGG C^[CT]CG[GA]G
AvaII 699 GGTCC G^G[AT]CC
AvrII 567 CCTAGG C^CTAGG
BanI 37 GGCACC G^G[CT][GA]CC
BbsI 1152 GTCTTC GAAGAC(2/6)
BfaI 568 CTAG C^TAG
BfrI 163 CTTAAG C^TTAAG
BglII 441 AGATCT A^GATCT
BlnI 567 CCTAGG C^CTAGG
BpmI 346 CTGGAG CTGGAG(16/14)
BpuAI 1152 GTCTTC GAAGAC(2/6)
BsaBI 204 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1242 GACGCC G[GA]^CG[CT]C
BsaI 241 GAGACC GGTCTC(1/5)
BsiHKAI 1119 GAGCTC G[AT]GC[AT]^C
BsmAI 241 GAGAC GTCTC(1/5)
BsmFI 864 GTCCC GGGAC(10/14)
BsmI 296 GCATTC GAATGC(1/-1)
BsoBI 27 CCCGGG C^[CT]CG[GA]G
Bsp1407I 494 TGTACA T^GTACA
BspHI 425 TCATGA T^CATGA
BspMI 379 GCAGGT ACCTGC(4/8)
BsrDI 1062 CATTGC GCAATG(2/0)
BsrGI 494 TGTACA T^GTACA
BstAPI 1210 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 1132 GCAAGC GC[ACGT]^[ACGT]GC
DraII 1259 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 392 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 273 CGGCCA [CT]^GGCC[GA]
EcoO109I 1259 AGGCCCC [GA]G^G[ACGT]CC[CT]
HapII 28 CCGG C^CGG
Hin1I 1242 GACGCC G[GA]^CG[CT]C
HpaII 28 CCGG C^CGG
MaeI 568 CTAG C^TAG
MamI 204 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 870 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 28 CCGG C^CGG
NspI 339 GCATGT [GA]CATG^[CT]
PflFI 1279 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 985 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 243 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 127 ATTAAT AT^TAAT
Psp1406I 184 AACGTT AA^CGTT
RcaI 425 TCATGA T^CATGA
SacI 1119 GAGCTC GAGCT^C
SapI 1121 GCTCTTC GCTCTTC(1/4)
SexAI 906 ACCAGGT A^CC[AT]GGT
SmaI 27 CCCGGG CCC^GGG
SmlI 163 CTTAAG C^T[CT][GA]AG
SspBI 494 TGTACA T^GTACA
Tth111I 1279 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 985 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 27 CCCGGG C^CCGGG
XmaI 27 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 24 GATCC GGATC(4/5)
168 GGATC
BanII 3 GGGCTC G[GA]GC[CT]^C
1119 GAGCTC
BcnI 27 CCCGG CC^[GC]GG
28 CCGGG
BseRI 6 CTCCTC GAGGAG(10/8)
1221 CTCCTC
Cfr13I 699 GGTCC G^G[ACGT]CC
1260 GGCCC
HaeIII 274 GGCC GG^CC
1260 GGCC
HphI 733 TCACC GGTGA(8/7)
1351 TCACC
MaeII 53 ACGT A^CGT
185 ACGT
MspA1I 703 CAGCTG C[AC]G^C[GT]G
797 CAGCTG
NciI 27 CCCGG CC^[GC]GG
28 CCGGG
PvuII 703 CAGCTG CAG^CTG
797 CAGCTG
Sau96I 699 GGTCC G^G[ACGT]CC
1260 GGCCC
TfiI 355 GAATC G^A[AT]TC
888 GAATC
XcmI 256 CCAAGTAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
538 CCAAATTCTGCATGG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HgaI HinfI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII