Restriction map for
pF1KB6968
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I Van91I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 330 GCGG CCGC(-3/-1)
AcsI 3 GAATTC [GA]^AATT[CT]
AlwNI 617 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 3 GAATTC [GA]^AATT[CT]
AvaII 129 GGTCC G^G[AT]CC
BbvI 673 GCAGC GCAGC(8/12)
BciVI 247 GTATCC GTATCC(6/5)
BfaI 548 CTAG C^TAG
BsaI 19 GAGACC GGTCTC(1/5)
BsiYI 57 CCAAGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 57 CCAAGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 19 GAGAC GTCTC(1/5)
BspMI 394 ACCTGC ACCTGC(4/8)
BstF5I 149 CATCC GGATG(2/0)
Cac8I 36 GCCTGC GC[ACGT]^[ACGT]GC
DraII 88 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 23 CCATGG C^C[GA][CT]GG
EaeI 216 TGGCCG [CT]^GGCC[GA]
EcoO109I 88 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3 GAATTC G^AATTC
Fnu4HI 673 GCAGC GC^[ACGT]GC
FokI 149 CATCC GGATG(9/13)
HindIII 208 AAGCTT A^AGCTT
ItaI 673 GCAGC GC^[ACGT]GC
MaeI 548 CTAG C^TAG
MaeIII 76 GTGAC ^GT[ACGT]AC
NcoI 23 CCATGG C^CATGG
NdeI 142 CATATG CA^TATG
NlaIV 88 GGGGCC GG[ACGT]^[ACGT]CC
SexAI 447 ACCAGGT A^CC[AT]GGT
SmlI 373 CTTGAG C^T[CT][GA]AG
StuI 13 AGGCCT AGG^CCT
TaqI 135 TCGA T^CGA
TfiI 540 GAATC G^A[AT]TC
TseI 673 GCAGC G^C[AT]GC
Tsp45I 76 GTGAC ^GT[GC]AC
Tsp509I 4 AATT ^AATT
TspRI 354 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 135 TCGA T^CGA
XbaI 547 TCTAGA T^CTAGA
XcmI 345 CCAGGATTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 10 enzymes) #
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AluI 209 AGCT AG^CT
613 AGCT
AlwI 295 GATCC GGATC(4/5)
338 GATCC
BsmFI 277 GGGAC GGGAC(10/14)
454 GGGAC
BsrI 280 ACTGG ACTGG(1/-1)
355 ACTGG
Cfr13I 89 GGGCC G^G[ACGT]CC
129 GGTCC
EcoT14I 23 CCATGG C^C[AT][AT]GG
57 CCAAGG
MwoI 410 GCTGTAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
502 GCAAAGTCAGC
PleI 604 GACTC GAGTC(4/5)
619 GACTC
Sau96I 89 GGGCC G^G[ACGT]CC
129 GGTCC
StyI 23 CCATGG C^C[AT][AT]GG
57 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
BsaJI BssKI BstNI DdeI DpnII
EarI EcoRII HaeIII HinfI HphI
Ksp632I MboI MboII MnlI MvaI
NdeII NlaIII Sau3AI ScrFI