Restriction map for
pF1KB6318
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NciI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 54 CGCG CG^CG
AlwNI 731 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 100 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 49 GAGCTC G[AT]GC[AT]^C
AviII 225 TGCGCA TGC^GCA
BbsI 100 GAAGAC GAAGAC(2/6)
BpmI 637 CTCCAG CTGGAG(16/14)
BpuAI 100 GAAGAC GAAGAC(2/6)
BsaI 81 GGTCTC GGTCTC(1/5)
BseRI 196 GAGGAG GAGGAG(10/8)
BsgI 117 CTGCAC GTGCAG(16/14)
BsiEI 159 CGGCCG CG[GA][CT]^CG
BsiHKAI 49 GAGCTC G[AT]GC[AT]^C
Bsp1407I 462 TGTACA T^GTACA
BsrBI 212 CCGCTC CCGCTC(-3/-3)
BsrDI 812 CATTGC GCAATG(2/0)
BsrGI 462 TGTACA T^GTACA
BstUI 54 CGCG CG^CG
BstYI 395 AGATCC [GA]^GATC[CT]
DsaI 37 CCATGG C^C[GA][CT]GG
EagI 159 CGGCCG C^GGCCG
EclXI 159 CGGCCG C^GGCCG
Eco52I 159 CGGCCG C^GGCCG
FspI 225 TGCGCA TGC^GCA
HapII 193 CCGG C^CGG
HgaI 184 GACGC GACGC(5/10)
HindIII 290 AAGCTT A^AGCTT
HpaII 193 CCGG C^CGG
HphI 648 TCACC GGTGA(8/7)
MaeIII 136 GTGAC ^GT[ACGT]AC
MflI 395 AGATCC [GA]^GATC[CT]
MspA1I 245 CAGCTG C[AC]G^C[GT]G
MspI 193 CCGG C^CGG
MvnI 54 CGCG CG^CG
NcoI 37 CCATGG C^CATGG
NdeI 505 CATATG CA^TATG
NlaIV 207 GGAGCC GG[ACGT]^[ACGT]CC
NotI 158 GCGGCCGC GC^GGCCGC
PflMI 72 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 245 CAGCTG CAG^CTG
SacI 49 GAGCTC GAGCT^C
ScaI 327 AGTACT AGT^ACT
SspBI 462 TGTACA T^GTACA
StuI 74 AGGCCT AGG^CCT
TaqI 124 TCGA T^CGA
Tsp45I 136 GTGAC ^GT[GC]AC
TthHB8I 124 TCGA T^CGA
Van91I 72 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 389 CCAGAAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 395 AGATCC [GA]^GATC[CT]
XmnI 100 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 437 AAATTC [GA]^AATT[CT]
513 AAATTT
AlwI 396 GATCC GGATC(4/5)
493 GATCC
ApoI 437 AAATTC [GA]^AATT[CT]
513 AAATTT
AvaI 95 CCCGAG C^[CT]CG[GA]G
178 CTCGGG
AvaII 24 GGTCC G^G[AT]CC
474 GGACC
BanII 49 GAGCTC G[GA]GC[CT]^C
208 GAGCCC
BbvI 621 GCAGC GCAGC(8/12)
116 GCTGC
BciVI 384 GTATCC GTATCC(6/5)
807 GTATCC
BmyI 49 GAGCTC G[AGT]GC[ACT]^C
208 GAGCCC
BsaJI 37 CCATGG C^C[ACGT][ACGT]GG
77 CCTTGG
BsmAI 733 GAGAC GTCTC(1/5)
82 GTCTC
BsmFI 41 GGGAC GGGAC(10/14)
182 GGGAC
BsoBI 95 CCCGAG C^[CT]CG[GA]G
178 CTCGGG
Bsp1286I 49 GAGCTC G[AGT]GC[ACT]^C
208 GAGCCC
BsrI 471 ACTGG ACTGG(1/-1)
665 CCAGT
BstF5I 466 CATCC GGATG(2/0)
617 GGATG
CfoI 114 GCGC GCG^C
226 GCGC
Cfr13I 24 GGTCC G^G[ACGT]CC
474 GGACC
EaeI 159 CGGCCG [CT]^GGCC[GA]
817 CGGCCA
EcoT14I 37 CCATGG C^C[AT][AT]GG
77 CCTTGG
FokI 466 CATCC GGATG(9/13)
617 GGATG
HhaI 114 GCGC GCG^C
226 GCGC
HinP1I 114 GCGC G^CGC
226 GCGC
MaeII 375 ACGT A^CGT
758 ACGT
MboII 100 GAAGA GAAGA(8/7)
334 GAAGA
Sau96I 24 GGTCC G^G[ACGT]CC
474 GGACC
StyI 37 CCATGG C^C[AT][AT]GG
77 CCTTGG
TseI 116 GCTGC G^C[AT]GC
621 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI AseI AsnI
BsiYI BslI BssKI BstNI Cac8I
DdeI DpnII EcoRII FauI Fnu4HI
HaeIII HinfI ItaI MboI MnlI
MseI MvaI MwoI NdeII NlaIII
PshBI RsaI Sau3AI ScrFI SfaNI
TfiI Tru9I Tsp509I TspRI