Restriction map for
pF1KB3452
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BfaI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT22I
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MroI MslI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 19 CGCG CG^CG
AfaI 30 GTAC GT^AC
AflII 163 CTTAAG C^TTAAG
AlwI 124 GATCC GGATC(4/5)
Aor51HI 140 AGCGCT AGC^GCT
AspHI 621 GAGCAC G[AT]GC[AT]^C
AvaI 147 CTCGGG C^[CT]CG[GA]G
AvaII 207 GGACC G^G[AT]CC
BanI 151 GGCACC G^G[CT][GA]CC
BbrPI 511 CACGTG CAC^GTG
BclI 347 TGATCA T^GATCA
BcnI 603 CCGGG CC^[GC]GG
BfrI 163 CTTAAG C^TTAAG
BpmI 85 CTGGAG CTGGAG(16/14)
BsaAI 511 CACGTG [CT]AC^GT[GA]
BsgI 52 CTGCAC GTGCAG(16/14)
BsiHKAI 621 GAGCAC G[AT]GC[AT]^C
BsmBI 88 GAGACG CGTCTC(1/5)
BsmFI 206 GGGAC GGGAC(10/14)
BsoBI 147 CTCGGG C^[CT]CG[GA]G
BsrDI 575 CATTGC GCAATG(2/0)
BstF5I 444 GGATG GGATG(2/0)
BstUI 19 CGCG CG^CG
DraI 381 TTTAAA TTT^AAA
Eco47III 140 AGCGCT AGC^GCT
EcoNI 27 CCTGTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 241 GATATC GAT^ATC
EcoT14I 626 CCATGG C^C[AT][AT]GG
FbaI 347 TGATCA T^GATCA
FokI 444 GGATG GGATG(9/13)
HapII 603 CCGG C^CGG
HincII 559 GTCAAC GT[CT]^[GA]AC
HindII 559 GTCAAC GT[CT]^[GA]AC
HpaII 603 CCGG C^CGG
MaeII 512 ACGT A^CGT
MspI 603 CCGG C^CGG
MvnI 19 CGCG CG^CG
NciI 603 CCGGG CC^[GC]GG
NcoI 626 CCATGG C^CATGG
NruI 18 TCGCGA TCG^CGA
PleI 340 GAGTC GAGTC(4/5)
PmaCI 511 CACGTG CAC^GTG
PmlI 511 CACGTG CAC^GTG
RsaI 30 GTAC GT^AC
SfaNI 322 GATGC GCATC(5/9)
SfcI 497 CTGTAG C^T[GA][CT]AG
StyI 626 CCATGG C^C[AT][AT]GG
TaqI 260 TCGA T^CGA
TspRI 217 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 260 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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BalI 308 TGGCCA TGG^CCA
434 TGGCCA
BbvI 5 GCAGC GCAGC(8/12)
213 GCAGC
BmyI 150 GGGCAC G[AGT]GC[ACT]^C
621 GAGCAC
Bsp1286I 150 GGGCAC G[AGT]GC[ACT]^C
621 GAGCAC
BsrI 294 ACTGG ACTGG(1/-1)
590 ACTGG
DsaI 236 CCGTGG C^C[GA][CT]GG
626 CCATGG
EaeI 308 TGGCCA [CT]^GGCC[GA]
434 TGGCCA
FauI 176 CCCGC CCCGC(4/6)
42 GCGGG
HphI 450 GGTGA GGTGA(8/7)
549 TCACC
MaeIII 221 GTGAC ^GT[ACGT]AC
509 GTCAC
MluNI 308 TGGCCA TGG^CCA
434 TGGCCA
MscI 308 TGGCCA TGG^CCA
434 TGGCCA
MseI 164 TTAA T^TAA
382 TTAA
SmlI 163 CTTAAG C^T[CT][GA]AG
580 CTCAAG
TfiI 357 GATTC G^A[AT]TC
365 GAATC
Tru9I 164 TTAA T^TAA
382 TTAA
TseI 5 GCAGC G^C[AT]GC
213 GCAGC
Tsp45I 221 GTGAC ^GT[GC]AC
509 GTCAC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsmAI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeII HaeIII HhaI HinP1I HinfI
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI