Restriction map for
pF1KB9933
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglII
BpuAI BsaHI BsaI BseAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI TfiI Tru9I Van91I XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 844 GTCTAC GT^[AC][GT]AC
AccII 88 CGCG CG^CG
AcsI 31 AAATTC [GA]^AATT[CT]
AocI 1386 CCTGAGG CC^T[ACGT]AGG
ApoI 31 AAATTC [GA]^AATT[CT]
Asp700I 921 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 184 GTGCTC G[AT]GC[AT]^C
AspI 283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 599 TGCGCA TGC^GCA
AvrII 1275 CCTAGG C^CTAGG
BalI 1331 TGGCCA TGG^CCA
BbvCI 1126 GCTGAGG CCTCAGC(-5/-2)
BfaI 1276 CTAG C^TAG
BlnI 1275 CCTAGG C^CTAGG
BlpI 575 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 575 GCTCAGC GC^T[ACGT]AGC
BsaAI 1186 TACGTG [CT]AC^GT[GA]
BsaBI 1161 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 875 TCCGGT [AT]^CCGG[AT]
BsiEI 1413 CGACCG CG[GA][CT]^CG
BsiHKAI 184 GTGCTC G[AT]GC[AT]^C
BstUI 88 CGCG CG^CG
BstYI 332 AGATCC [GA]^GATC[CT]
Bsu36I 1386 CCTGAGG CC^T[ACGT]AGG
CelII 575 GCTCAGC GC^T[ACGT]AGC
DrdI 310 GACTGCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1331 TGGCCA [CT]^GGCC[GA]
EarI 1072 CTCTTC CTCTTC(1/4)
Eco81I 1386 CCTGAGG CC^T[ACGT]AGG
EcoNI 1071 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 599 TGCGCA TGC^GCA
HincII 127 GTCAAC GT[CT]^[GA]AC
HindII 127 GTCAAC GT[CT]^[GA]AC
Ksp632I 1072 CTCTTC CTCTTC(1/4)
MaeI 1276 CTAG C^TAG
MamI 1161 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 332 AGATCC [GA]^GATC[CT]
MluNI 1331 TGGCCA TGG^CCA
MscI 1331 TGGCCA TGG^CCA
MvnI 88 CGCG CG^CG
PflFI 283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 781 CTGCAG CTGCA^G
SbfI 780 CCTGCAGG CCTGCA^GG
SfcI 781 CTGCAG C^T[GA][CT]AG
SmaI 1260 CCCGGG CCC^GGG
Sse8387I 780 CCTGCAGG CCTGCA^GG
Tsp45I 863 GTGAC ^GT[GC]AC
Tsp509I 32 AATT ^AATT
Tth111I 283 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 332 AGATCC [GA]^GATC[CT]
XmaCI 1260 CCCGGG C^CCGGG
XmaI 1260 CCCGGG C^CCGGG
XmnI 921 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 923 ACACGT A^C[GA][CT]GT
1229 ACGTGT
AlwI 333 GATCC GGATC(4/5)
1161 GATCC
AlwNI 784 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1372 CAGGAGCTG
BspHI 651 TCATGA T^CATGA
1046 TCATGA
BstAPI 113 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1371 GCAGGAGCTGC
HaeII 741 GGCGCT [GA]GCGC^[CT]
1392 GGCGCT
HinfI 793 GACTC G^A[ACGT]TC
865 GACTC
MaeIII 100 GTTAC ^GT[ACGT]AC
863 GTGAC
NaeI 1220 GCCGGC GCC^GGC
1244 GCCGGC
NcoI 773 CCATGG C^CATGG
1253 CCATGG
NgoMIV 1220 GCCGGC G^CCGGC
1244 GCCGGC
PaeR7I 763 CTCGAG C^TCGAG
795 CTCGAG
PleI 793 GACTC GAGTC(4/5)
865 GACTC
PshAI 265 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
610 GACCCCCGTC
RcaI 651 TCATGA T^CATGA
1046 TCATGA
SmlI 763 CTCGAG C^T[CT][GA]AG
795 CTCGAG
SphI 580 GCATGC GCATG^C
962 GCATGC
StuI 218 AGGCCT AGG^CCT
1181 AGGCCT
XcmI 968 CCAAGCGGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1319 CCAGCGGCAGCCTGG
XhoI 763 CTCGAG C^TCGAG
795 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I