##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HphI        
KasI        KpnI        KspI        MamI        MluI        
MroI        MslI        MspA1I      MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AlwNI         301  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       343  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         500  TGCGCA               TGC^GCA
BalI          150  TGGCCA               TGG^CCA
BcnI           78  CCCGG                CC^[GC]GG
BfaI          448  CTAG                 C^TAG
BsmAI         288  GTCTC                GTCTC(1/5)
BsmFI         237  GTCCC                GGGAC(10/14)
BsrDI          13  GCAATG               GCAATG(2/0)
BsrI          261  ACTGG                ACTGG(1/-1)
BssSI         334  CACGAG               CACGAG(-5/-1)
BstYI         248  AGATCC               [GA]^GATC[CT]
Cac8I         486  GCATGC               GC[ACGT]^[ACGT]GC
CfoI          501  GCGC                 GCG^C
Cfr13I        297  GGCCC                G^G[ACGT]CC
EaeI          150  TGGCCA               [CT]^GGCC[GA]
EarI          324  GAAGAG               CTCTTC(1/4)
EcoNI         348  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          500  TGCGCA               TGC^GCA
HapII          79  CCGG                 C^CGG
HhaI          501  GCGC                 GCG^C
HinP1I        501  GCGC                 G^CGC
HpaII          79  CCGG                 C^CGG
Ksp632I       324  GAAGAG               CTCTTC(1/4)
MaeI          448  CTAG                 C^TAG
MaeIII         54  GTCAC                ^GT[ACGT]AC
MfeI          313  CAATTG               C^AATTG
MflI          248  AGATCC               [GA]^GATC[CT]
MluNI         150  TGGCCA               TGG^CCA
MscI          150  TGGCCA               TGG^CCA
MseI          425  TTAA                 T^TAA
MspI           79  CCGG                 C^CGG
MunI          313  CAATTG               C^AATTG
NciI           78  CCCGG                CC^[GC]GG
NlaIV          81  GGAACC               GG[ACGT]^[ACGT]CC
NspI          486  GCATGC               [GA]CATG^[CT]
Sau96I        297  GGCCC                G^G[ACGT]CC
SphI          486  GCATGC               GCATG^C
Tru9I         425  TTAA                 T^TAA
Tsp45I         54  GTCAC                ^GT[GC]AC
TspRI          56  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         248  AGATCC               [GA]^GATC[CT]
XmnI          343  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI          391  GCGG                 CCGC(-3/-1)
              493  GCGG                
AlwI          249  GATCC                GGATC(4/5)
              405  GATCC               
BsaJI         239  CCCTGG               C^C[ACGT][ACGT]GG
              299  CCCAGG              
BseRI         244  GAGGAG               GAGGAG(10/8)
              364  GAGGAG              
DdeI          192  CTCAG                C^T[ACGT]AG
              290  CTCAG               
MaeII         141  ACGT                 A^CGT
              465  ACGT                
NlaIII         48  CATG                 CATG^
              487  CATG                
PstI          269  CTGCAG               CTGCA^G
              469  CTGCAG              
SfcI          269  CTGCAG               C^T[GA][CT]AG
              469  CTGCAG              
Tsp509I       314  AATT                 ^AATT
              423  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        BbvI        BpmI        BsiYI       BslI        
BssKI       BstNI       DpnII       EcoRII      Fnu4HI      
HaeIII      ItaI        MboI        MboII       MnlI        
MvaI        MwoI        NdeII       Sau3AI      ScrFI       
TseI