Restriction map for
pF1KB5055
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AvaI AviII
AvrII BbeI BbrPI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI ScaI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 1504 GGCGTC G[GA]^CG[CT]C
AhdI 1345 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1345 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 211 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1214 GGATCC G^GATCC
BanI 1414 GGTGCC G^G[CT][GA]CC
BanII 1158 GGGCTC G[GA]GC[CT]^C
BbsI 547 GAAGAC GAAGAC(2/6)
BcgI 126 CGACTTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 422 GTATCC GTATCC(6/5)
BclI 857 TGATCA T^GATCA
BfaI 703 CTAG C^TAG
BglI 1132 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 477 CTCCAG CTGGAG(16/14)
BpuAI 547 GAAGAC GAAGAC(2/6)
BsaAI 34 TACGTG [CT]AC^GT[GA]
BsaHI 1504 GGCGTC G[GA]^CG[CT]C
BsaWI 1077 TCCGGT [AT]^CCGG[AT]
BsgI 1339 GTGCAG GTGCAG(16/14)
BsmAI 80 GAGAC GTCTC(1/5)
BsmBI 80 GAGACG CGTCTC(1/5)
BspMI 946 GCAGGT ACCTGC(4/8)
BsrI 192 ACTGG ACTGG(1/-1)
BstAPI 999 GCAGGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1214 GGATCC [GA]^GATC[CT]
DrdI 349 GACCCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1345 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 292 GAATTC G^AATTC
FbaI 857 TGATCA T^GATCA
HgaI 1505 GCGTC GACGC(5/10)
Hin1I 1504 GGCGTC G[GA]^CG[CT]C
MaeI 703 CTAG C^TAG
MflI 1214 GGATCC [GA]^GATC[CT]
MspA1I 727 CAGCTG C[AC]G^C[GT]G
PflFI 211 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1101 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1428 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 727 CAGCTG CAG^CTG
SapI 66 GCTCTTC GCTCTTC(1/4)
SbfI 565 CCTGCAGG CCTGCA^GG
SexAI 140 ACCAGGT A^CC[AT]GGT
SfiI 1131 GGCCCTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 565 CCTGCAGG CCTGCA^GG
Tth111I 211 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1101 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1214 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 376 CGCG CG^CG
1234 CGCG
AflII 181 CTTAAG C^TTAAG
1462 CTTAAG
AvaII 147 GGACC G^G[AT]CC
357 GGTCC
BalI 233 TGGCCA TGG^CCA
656 TGGCCA
BcnI 404 CCCGG CC^[GC]GG
668 CCCGG
BfrI 181 CTTAAG C^TTAAG
1462 CTTAAG
BmyI 24 GGGCAC G[AGT]GC[ACT]^C
1158 GGGCTC
BseRI 474 CTCCTC GAGGAG(10/8)
502 GAGGAG
Bsp1286I 24 GGGCAC G[AGT]GC[ACT]^C
1158 GGGCTC
Bsp1407I 283 TGTACA T^GTACA
1040 TGTACA
BspHI 756 TCATGA T^CATGA
1151 TCATGA
BsrGI 283 TGTACA T^GTACA
1040 TGTACA
BstUI 376 CGCG CG^CG
1234 CGCG
DraII 1138 GGGGCCT [GA]G^G[ACGT]CC[CT]
1184 AGGCCCC
DsaI 206 CCATGG C^C[GA][CT]GG
1256 CCATGG
EcoO109I 1138 GGGGCCT [GA]G^G[ACGT]CC[CT]
1184 AGGCCCC
FauI 381 GCGGG CCCGC(4/6)
963 GCGGG
HaeII 246 GGCGCT [GA]GCGC^[CT]
1064 GGCGCT
HphI 1192 GGTGA GGTGA(8/7)
89 TCACC
MaeIII 217 GTCAC ^GT[ACGT]AC
913 GTCAC
MluNI 233 TGGCCA TGG^CCA
656 TGGCCA
MscI 233 TGGCCA TGG^CCA
656 TGGCCA
MseI 182 TTAA T^TAA
1463 TTAA
MvnI 376 CGCG CG^CG
1234 CGCG
NciI 404 CCCGG CC^[GC]GG
668 CCCGG
NcoI 206 CCATGG C^CATGG
1256 CCATGG
PleI 50 GACTC GAGTC(4/5)
820 GACTC
RcaI 756 TCATGA T^CATGA
1151 TCATGA
SmlI 181 CTTAAG C^T[CT][GA]AG
1462 CTTAAG
SspBI 283 TGTACA T^GTACA
1040 TGTACA
TaqI 125 TCGA T^CGA
1250 TCGA
Tru9I 182 TTAA T^TAA
1463 TTAA
Tsp45I 217 GTCAC ^GT[GC]AC
913 GTCAC
TthHB8I 125 TCGA T^CGA
1250 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BbvCI BbvI BsaJI
BsiYI BslI BsmFI BsrDI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeII MboI MboII
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI Tsp509I TspRI
XcmI