Restriction map for
pF1KE0902
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 123 GAATTT [GA]^AATT[CT]
AlwI 380 GATCC GGATC(4/5)
AocI 232 CCTGAGG CC^T[ACGT]AGG
ApaI 297 GGGCCC GGGCC^C
ApoI 123 GAATTT [GA]^AATT[CT]
AvaI 289 CTCGGG C^[CT]CG[GA]G
AvrII 57 CCTAGG C^CTAGG
BbvCI 342 GCTGAGG CCTCAGC(-5/-2)
BcnI 251 CCGGG CC^[GC]GG
BfaI 58 CTAG C^TAG
BlnI 57 CCTAGG C^CTAGG
BsaWI 446 TCCGGT [AT]^CCGG[AT]
BseRI 309 CTCCTC GAGGAG(10/8)
BsmFI 305 GGGAC GGGAC(10/14)
BsmI 46 GCATTC GAATGC(1/-1)
BsoBI 289 CTCGGG C^[CT]CG[GA]G
Bsp1407I 83 TGTACA T^GTACA
BspDI 317 ATCGAT AT^CGAT
BsrBI 423 GAGCGG CCGCTC(-3/-3)
BsrGI 83 TGTACA T^GTACA
BstF5I 401 GGATG GGATG(2/0)
BstXI 270 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 379 AGATCC [GA]^GATC[CT]
Bsu36I 232 CCTGAGG CC^T[ACGT]AGG
CfoI 349 GCGC GCG^C
ClaI 317 ATCGAT AT^CGAT
Eco81I 232 CCTGAGG CC^T[ACGT]AGG
EcoNI 228 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 57 CCTAGG C^C[AT][AT]GG
FokI 401 GGATG GGATG(9/13)
HaeII 348 GGCGCT [GA]GCGC^[CT]
HaeIII 298 GGCC GG^CC
HhaI 349 GCGC GCG^C
HinP1I 349 GCGC G^CGC
HinfI 307 GACTC G^A[ACGT]TC
MaeI 58 CTAG C^TAG
MaeIII 437 GTAAC ^GT[ACGT]AC
MflI 379 AGATCC [GA]^GATC[CT]
MslI 18 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 384 CAGCTG C[AC]G^C[GT]G
NciI 251 CCGGG CC^[GC]GG
NlaIII 81 CATG CATG^
PleI 307 GACTC GAGTC(4/5)
PvuII 384 CAGCTG CAG^CTG
SacI 3 GAGCTC GAGCT^C
SfaNI 150 GATGC GCATC(5/9)
SspBI 83 TGTACA T^GTACA
StyI 57 CCTAGG C^C[AT][AT]GG
TaqI 318 TCGA T^CGA
Tsp509I 124 AATT ^AATT
TthHB8I 318 TCGA T^CGA
XhoII 379 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 84 GTAC GT^AC
450 GTAC
AspHI 3 GAGCTC G[AT]GC[AT]^C
184 GTGCTC
AvaII 132 GGACC G^G[AT]CC
248 GGACC
BanI 237 GGTGCC G^G[CT][GA]CC
283 GGTGCC
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
184 GTGCTC
BsrI 42 ACTGG ACTGG(1/-1)
429 ACTGG
BstNI 245 CCTGG CC^[AT]GG
302 CCTGG
Cac8I 338 GCAAGC GC[ACGT]^[ACGT]GC
371 GCCTGC
DraII 296 GGGGCCC [GA]G^G[ACGT]CC[CT]
297 GGGCCCC
EcoO109I 296 GGGGCCC [GA]G^G[ACGT]CC[CT]
297 GGGCCCC
EcoRII 245 CCTGG ^CC[AT]GG
302 CCTGG
FauI 208 GCGGG CCCGC(4/6)
294 GCGGG
HapII 251 CCGG C^CGG
447 CCGG
HpaII 251 CCGG C^CGG
447 CCGG
MspI 251 CCGG C^CGG
447 CCGG
MvaI 245 CCTGG CC^[AT]GG
302 CCTGG
RsaI 84 GTAC GT^AC
450 GTAC
TspRI 189 CAGTG CA[GC]TG[ACGT][ACGT]^
357 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
Cfr13I DdeI DpnII Fnu4HI ItaI
MboI MboII MnlI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI