##################################
# NO CUT ENZYMES ( 195 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MboI MfeI MflI MluI MroI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AciI 4 CCGC CCGC(-3/-1)
AlwNI 356 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 7 CCTAAGG CC^T[ACGT]AGG
AvaII 104 GGTCC G^G[AT]CC
BalI 248 TGGCCA TGG^CCA
BcnI 77 CCGGG CC^[GC]GG
BpmI 353 CTCCAG CTGGAG(16/14)
BsaI 216 GGTCTC GGTCTC(1/5)
BsmFI 122 GGGAC GGGAC(10/14)
Bsu36I 7 CCTAAGG CC^T[ACGT]AGG
Cfr13I 104 GGTCC G^G[ACGT]CC
Eco81I 7 CCTAAGG CC^T[ACGT]AGG
EcoT14I 338 CCAAGG C^C[AT][AT]GG
HapII 77 CCGG C^CGG
HgaI 31 GACGC GACGC(5/10)
HinfI 234 GATTC G^A[ACGT]TC
HpaII 77 CCGG C^CGG
HphI 364 GGTGA GGTGA(8/7)
MluNI 248 TGGCCA TGG^CCA
MnlI 239 GAGG CCTC(7/6)
MscI 248 TGGCCA TGG^CCA
MspI 77 CCGG C^CGG
NciI 77 CCGGG CC^[GC]GG
NlaIV 241 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 355 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 104 GGTCC G^G[ACGT]CC
SmlI 314 CTCAAG C^T[CT][GA]AG
StyI 338 CCAAGG C^C[AT][AT]GG
TaqI 237 TCGA T^CGA
TfiI 234 GATTC G^A[AT]TC
Tsp509I 321 AATT ^AATT
TspRI 65 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 237 TCGA T^CGA
Van91I 355 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 268 GAGCTC G[AT]GC[AT]^C
311 GAGCTC
BanII 268 GAGCTC G[GA]GC[CT]^C
311 GAGCTC
BbvI 256 GCAGC GCAGC(8/12)
358 GCTGC
BmyI 268 GAGCTC G[AGT]GC[ACT]^C
311 GAGCTC
BsiHKAI 268 GAGCTC G[AT]GC[AT]^C
311 GAGCTC
BsmAI 225 GAGAC GTCTC(1/5)
217 GTCTC
Bsp1286I 268 GAGCTC G[AGT]GC[ACT]^C
311 GAGCTC
BsrI 294 ACTGG ACTGG(1/-1)
64 CCAGT
BstNI 246 CCTGG CC^[AT]GG
251 CCAGG
EaeI 44 CGGCCA [CT]^GGCC[GA]
248 TGGCCA
EcoRII 246 CCTGG ^CC[AT]GG
251 CCAGG
MspA1I 209 CAGCTG C[AC]G^C[GT]G
356 CAGCTG
MvaI 246 CCTGG CC^[AT]GG
251 CCAGG
MwoI 250 GCCAGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
352 GCTCCAGCTGC
PvuII 209 CAGCTG CAG^CTG
356 CAGCTG
SacI 268 GAGCTC GAGCT^C
311 GAGCTC
SfaNI 349 GATGC GCATC(5/9)
370 GATGC
TseI 256 GCAGC G^C[AT]GC
358 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BssKI
DdeI Fnu4HI HaeIII ItaI MboII
ScrFI