Restriction map for
pF1KB6836
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstF5I BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
TspRI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AcyI 283 GACGCC G[GA]^CG[CT]C
AfaI 69 GTAC GT^AC
AlwI 336 GGATC GGATC(4/5)
AspHI 3 GAGCTC G[AT]GC[AT]^C
AvaII 366 GGACC G^G[AT]CC
BanII 3 GAGCTC G[GA]GC[CT]^C
BcnI 471 CCCGG CC^[GC]GG
BfaI 403 CTAG C^TAG
BmyI 3 GAGCTC G[AGT]GC[ACT]^C
BsaHI 283 GACGCC G[GA]^CG[CT]C
BseRI 169 GAGGAG GAGGAG(10/8)
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsmBI 193 CGTCTC CGTCTC(1/5)
Bsp1286I 3 GAGCTC G[AGT]GC[ACT]^C
BspMI 71 ACCTGC ACCTGC(4/8)
BstBI 499 TTCGAA TT^CGAA
BstEII 436 GGTGACC G^GT[ACGT]ACC
BstPI 436 GGTGACC G^GT[ACGT]ACC
BstYI 336 GGATCT [GA]^GATC[CT]
DpnII 337 GATC ^GATC
DraII 326 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 299 CCATGG C^C[GA][CT]GG
EagI 222 CGGCCG C^GGCCG
EclXI 222 CGGCCG C^GGCCG
Eco52I 222 CGGCCG C^GGCCG
EcoNI 402 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 326 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 436 GGTGACC G^GT[ACGT]ACC
EcoT14I 299 CCATGG C^C[AT][AT]GG
FauI 359 CCCGC CCCGC(4/6)
HapII 472 CCGG C^CGG
Hin1I 283 GACGCC G[GA]^CG[CT]C
HincII 49 GTCAAC GT[CT]^[GA]AC
HindII 49 GTCAAC GT[CT]^[GA]AC
HindIII 418 AAGCTT A^AGCTT
HpaII 472 CCGG C^CGG
HphI 436 GGTGA GGTGA(8/7)
MaeI 403 CTAG C^TAG
MaeII 35 ACGT A^CGT
MboI 337 GATC ^GATC
MflI 336 GGATCT [GA]^GATC[CT]
MspA1I 254 CAGCTG C[AC]G^C[GT]G
MspI 472 CCGG C^CGG
NciI 471 CCCGG CC^[GC]GG
NcoI 299 CCATGG C^CATGG
NdeII 337 GATC ^GATC
NlaIV 366 GGACCC GG[ACGT]^[ACGT]CC
NotI 221 GCGGCCGC GC^GGCCGC
NspV 499 TTCGAA TT^CGAA
PflMI 456 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 393 GACTC GAGTC(4/5)
PstI 73 CTGCAG CTGCA^G
PvuII 254 CAGCTG CAG^CTG
RsaI 69 GTAC GT^AC
SacI 3 GAGCTC GAGCT^C
Sau3AI 337 GATC ^GATC
SbfI 72 CCTGCAGG CCTGCA^GG
SfaNI 204 GATGC GCATC(5/9)
SfcI 73 CTGCAG C^T[GA][CT]AG
SfuI 499 TTCGAA TT^CGAA
SmlI 514 CTCAAG C^T[CT][GA]AG
Sse8387I 72 CCTGCAGG CCTGCA^GG
StuI 77 AGGCCT AGG^CCT
StyI 299 CCATGG C^C[AT][AT]GG
TfiI 12 GATTC G^A[AT]TC
Van91I 456 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 456 CCACAGGCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 336 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbvI 43 GCAGC GCAGC(8/12)
295 GCTGC
BpmI 307 CTGGAG CTGGAG(16/14)
388 CTGGAG
BsiEI 114 CGACCG CG[GA][CT]^CG
222 CGGCCG
BsmAI 391 GAGAC GTCTC(1/5)
194 GTCTC
BstXI 29 CCACCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
299 CCATGGCCCTGG
CfoI 177 GCGC GCG^C
227 GCGC
EaeI 164 TGGCCG [CT]^GGCC[GA]
222 CGGCCG
EarI 232 CTCTTC CTCTTC(1/4)
496 CTCTTC
HgaI 283 GACGC GACGC(5/10)
192 GCGTC
HhaI 177 GCGC GCG^C
227 GCGC
HinP1I 177 GCGC G^CGC
227 GCGC
HinfI 12 GATTC G^A[ACGT]TC
393 GACTC
Ksp632I 232 CTCTTC CTCTTC(1/4)
496 CTCTTC
MaeIII 380 GTGAC ^GT[ACGT]AC
437 GTGAC
MslI 30 CACCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
373 CATCTCTGTG
TaqI 113 TCGA T^CGA
500 TCGA
TseI 43 GCAGC G^C[AT]GC
295 GCTGC
Tsp45I 380 GTGAC ^GT[GC]AC
437 GTGAC
TthHB8I 113 TCGA T^CGA
500 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AccII AciI AluI BsaJI BsiYI
BslI BssKI BstNI BstUI Cac8I
Cfr13I EcoRII Fnu4HI HaeIII ItaI
MboII MnlI MvaI MvnI MwoI
NlaIII Sau96I ScrFI Tsp509I