Restriction map for
pF1KE1004
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaJI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DpnII DraI DraII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AspI 382 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 206 CCGGG CC^[GC]GG
BpmI 29 CTGGAG CTGGAG(16/14)
BsaI 32 GAGACC GGTCTC(1/5)
BseRI 289 GAGGAG GAGGAG(10/8)
BsgI 137 GTGCAG GTGCAG(16/14)
BsmAI 32 GAGAC GTCTC(1/5)
BstF5I 276 CATCC GGATG(2/0)
DdeI 23 CTAAG C^T[ACGT]AG
DraIII 343 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 203 TGGCCG [CT]^GGCC[GA]
FokI 276 CATCC GGATG(9/13)
HapII 206 CCGG C^CGG
HincII 226 GTCAAC GT[CT]^[GA]AC
HindII 226 GTCAAC GT[CT]^[GA]AC
HpaII 206 CCGG C^CGG
MboII 180 GAAGA GAAGA(8/7)
MspI 206 CCGG C^CGG
NciI 206 CCGGG CC^[GC]GG
NlaIV 108 GGCCCC GG[ACGT]^[ACGT]CC
PflFI 382 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 264 CTGCAG CTGCA^G
ScaI 248 AGTACT AGT^ACT
SmlI 462 CTCAAG C^T[CT][GA]AG
Tsp509I 188 AATT ^AATT
Tth111I 382 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 219 GTAC GT^AC
249 GTAC
AlwNI 63 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
128 CAGGAGCTG
AspHI 310 GTGCTC G[AT]GC[AT]^C
340 GAGCAC
BmyI 310 GTGCTC G[AGT]GC[ACT]^C
340 GAGCAC
BsiHKAI 310 GTGCTC G[AT]GC[AT]^C
340 GAGCAC
BsiYI 22 CCTAAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCAGCGGGCGG
BslI 22 CCTAAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCAGCGGGCGG
Bsp1286I 310 GTGCTC G[AGT]GC[ACT]^C
340 GAGCAC
BstAPI 303 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
409 GCAAGCGTTGC
CfoI 7 GCGC GCG^C
360 GCGC
Cfr13I 71 GGGCC G^G[ACGT]CC
108 GGCCC
FauI 374 CCCGC CCCGC(4/6)
282 GCGGG
HhaI 7 GCGC GCG^C
360 GCGC
HinP1I 7 GCGC G^CGC
360 GCGC
HinfI 368 GAGTC G^A[ACGT]TC
452 GAGTC
MaeIII 212 GTGAC ^GT[ACGT]AC
380 GTGAC
MspA1I 256 CCGCTG C[AC]G^C[GT]G
280 CAGCGG
PleI 368 GAGTC GAGTC(4/5)
452 GAGTC
RsaI 219 GTAC GT^AC
249 GTAC
Sau96I 71 GGGCC G^G[ACGT]CC
108 GGCCC
SexAI 221 ACCAGGT A^CC[AT]GGT
383 ACCAGGT
SfcI 60 CTACAG C^T[GA][CT]AG
264 CTGCAG
Tsp45I 212 GTGAC ^GT[GC]AC
380 GTGAC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI BbvI BsrI BssKI
BstNI Cac8I EcoRII Fnu4HI HaeIII
ItaI MnlI MvaI MwoI NlaIII
ScrFI TseI TspRI