Restriction map for
pF1KE1095
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
HaeII HgaI Hin1I HindIII HpaI
KasI Ksp632I KspI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 640 GGTACC G^GTACC
AflIII 1046 ACGTGT A^C[GA][CT]GT
AlwNI 608 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 640 GGTACC G^GTACC
AspHI 525 GAGCAC G[AT]GC[AT]^C
BbsI 1006 GTCTTC GAAGAC(2/6)
BbvCI 623 CCTCAGC CCTCAGC(-5/-2)
BciVI 801 GGATAC GTATCC(6/5)
BfaI 475 CTAG C^TAG
BglII 227 AGATCT A^GATCT
BpuAI 1006 GTCTTC GAAGAC(2/6)
BsaAI 868 TACGTG [CT]AC^GT[GA]
BsaBI 342 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 169 CTGCAC GTGCAG(16/14)
BsiEI 936 CGGTCG CG[GA][CT]^CG
BsiHKAI 525 GAGCAC G[AT]GC[AT]^C
BspMI 138 GCAGGT ACCTGC(4/8)
BsrDI 969 CATTGC GCAATG(2/0)
BssSI 133 CACGAG CACGAG(-5/-1)
BstXI 179 CCAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 227 AGATCT [GA]^GATC[CT]
DsaI 71 CCATGG C^C[GA][CT]GG
FauI 806 CCCGC CCCGC(4/6)
HincII 958 GTCAAC GT[CT]^[GA]AC
HindII 958 GTCAAC GT[CT]^[GA]AC
KpnI 640 GGTACC GGTAC^C
MaeI 475 CTAG C^TAG
MamI 342 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 227 AGATCT [GA]^GATC[CT]
MseI 728 TTAA T^TAA
NcoI 71 CCATGG C^CATGG
NspI 326 GCATGC [GA]CATG^[CT]
PpuMI 988 GGGACCT [GA]G^G[AT]CC[CT]
SfcI 614 CTGTAG C^T[GA][CT]AG
SmlI 796 CTCAAG C^T[CT][GA]AG
SphI 326 GCATGC GCATG^C
Tru9I 728 TTAA T^TAA
XhoII 227 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 825 CGCG CG^CG
835 CGCG
AviII 86 TGCGCA TGC^GCA
323 TGCGCA
BanI 538 GGCACC G^G[CT][GA]CC
640 GGTACC
BanII 103 GAGCCC G[GA]GC[CT]^C
436 GGGCTC
BpmI 289 CTGGAG CTGGAG(16/14)
460 CTGGAG
BseRI 744 CTCCTC GAGGAG(10/8)
20 GAGGAG
BsmAI 76 GAGAC GTCTC(1/5)
594 GTCTC
BsrBI 174 CCGCTC CCGCTC(-3/-3)
819 CCGCTC
BstUI 825 CGCG CG^CG
835 CGCG
DraII 849 GGGGCCT [GA]G^G[ACGT]CC[CT]
988 GGGACCT
EcoO109I 849 GGGGCCT [GA]G^G[ACGT]CC[CT]
988 GGGACCT
FspI 86 TGCGCA TGC^GCA
323 TGCGCA
HphI 296 TCACC GGTGA(8/7)
302 TCACC
MboII 357 GAAGA GAAGA(8/7)
1007 TCTTC
MvnI 825 CGCG CG^CG
835 CGCG
TaqI 18 TCGA T^CGA
1043 TCGA
TthHB8I 18 TCGA T^CGA
1043 TCGA
XcmI 272 CCAAGGAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
671 CCAAGGAGAAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeII MaeIII MboI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI StyI TseI Tsp45I TspRI
XmaCI XmaI