Restriction map for
pF1KE0004
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI SwaI Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 62 CGCG CG^CG
AcyI 621 GGCGTC G[GA]^CG[CT]C
AocI 1176 CCTGAGG CC^T[ACGT]AGG
ApaI 1266 GGGCCC GGGCC^C
AspHI 184 GAGCAC G[AT]GC[AT]^C
AviII 885 TGCGCA TGC^GCA
AvrII 1077 CCTAGG C^CTAGG
BamHI 794 GGATCC G^GATCC
BlnI 1077 CCTAGG C^CTAGG
BsaAI 303 CACGTA [CT]AC^GT[GA]
BsaHI 621 GGCGTC G[GA]^CG[CT]C
BsgI 529 GTGCAG GTGCAG(16/14)
BsiHKAI 184 GAGCAC G[AT]GC[AT]^C
BsmI 847 GAATGC GAATGC(1/-1)
BspLU11I 1289 ACATGT A^CATGT
BsrDI 359 CATTGC GCAATG(2/0)
BsrFI 1222 GCCGGC [GA]^CCGG[CT]
BstUI 62 CGCG CG^CG
Bsu36I 1176 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1222 GCCGGC [GA]^CCGG[CT]
DsaI 509 CCATGG C^C[GA][CT]GG
EaeI 425 TGGCCG [CT]^GGCC[GA]
EarI 741 CTCTTC CTCTTC(1/4)
Eco81I 1176 CCTGAGG CC^T[ACGT]AGG
FspI 885 TGCGCA TGC^GCA
HaeII 1213 AGCGCC [GA]GCGC^[CT]
HgaI 622 GCGTC GACGC(5/10)
Hin1I 621 GGCGTC G[GA]^CG[CT]C
Ksp632I 741 CTCTTC CTCTTC(1/4)
MseI 110 TTAA T^TAA
MslI 187 CACTCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 62 CGCG CG^CG
NaeI 1222 GCCGGC GCC^GGC
NcoI 509 CCATGG C^CATGG
NgoMIV 1222 GCCGGC G^CCGGC
SbfI 933 CCTGCAGG CCTGCA^GG
Sse8387I 933 CCTGCAGG CCTGCA^GG
TfiI 3 GATTC G^A[AT]TC
Tru9I 110 TTAA T^TAA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AvaI 181 CCCGAG C^[CT]CG[GA]G
468 CTCGGG
BanI 928 GGCACC G^G[CT][GA]CC
940 GGCACC
BclI 570 TGATCA T^GATCA
860 TGATCA
BsaWI 455 TCCGGT [AT]^CCGG[AT]
1110 ACCGGA
BseRI 440 CTCCTC GAGGAG(10/8)
68 GAGGAG
BsoBI 181 CCCGAG C^[CT]CG[GA]G
468 CTCGGG
BspMI 173 ACCTGC ACCTGC(4/8)
1376 GCAGGT
BstYI 794 GGATCC [GA]^GATC[CT]
1436 AGATCC
FauI 1275 CCCGC CCCGC(4/6)
1415 CCCGC
FbaI 570 TGATCA T^GATCA
860 TGATCA
HincII 759 GTTGAC GT[CT]^[GA]AC
1285 GTCAAC
HindII 759 GTTGAC GT[CT]^[GA]AC
1285 GTCAAC
HphI 198 GGTGA GGTGA(8/7)
726 GGTGA
MaeIII 727 GTGAC ^GT[ACGT]AC
1316 GTCAC
MflI 794 GGATCC [GA]^GATC[CT]
1436 AGATCC
NspI 755 GCATGT [GA]CATG^[CT]
1289 ACATGT
PflMI 693 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
837 CCAGCCTGTGG
PpuMI 142 AGGTCCT [GA]G^G[AT]CC[CT]
1181 GGGTCCC
PstI 503 CTGCAG CTGCA^G
934 CTGCAG
SfaNI 436 GATGC GCATC(5/9)
499 GATGC
StuI 314 AGGCCT AGG^CCT
1005 AGGCCT
Tsp45I 727 GTGAC ^GT[GC]AC
1316 GTCAC
Van91I 693 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
837 CCAGCCTGTGG
XcmI 944 CCAAGCTACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1326 CCAGCAGGCCCCTGG
XhoII 794 GGATCC [GA]^GATC[CT]
1436 AGATCC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MaeII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TseI TspRI TthHB8I