##################################
# NO CUT ENZYMES (  211 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoRV       EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MspI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AciI          103  GCGG                 CCGC(-3/-1)
AcsI          256  GAATTC               [GA]^AATT[CT]
AfaI           88  GTAC                 GT^AC
AluI           69  AGCT                 AG^CT
ApoI          256  GAATTC               [GA]^AATT[CT]
BanI           78  GGCACC               G^G[CT][GA]CC
BsaJI         201  CCAAGG               C^C[ACGT][ACGT]GG
BsiYI         245  CCATGTATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          245  CCATGTATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI         220  GCAATG               GCAATG(2/0)
BstAPI        115  GCAATTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI          62  TTCGAA               TT^CGAA
DdeI          272  CTGAG                C^T[ACGT]AG
EarI          144  GAAGAG               CTCTTC(1/4)
EcoRI         256  GAATTC               G^AATTC
EcoT14I       201  CCAAGG               C^C[AT][AT]GG
FauI          103  GCGGG                CCCGC(4/6)
HincII          3  GTCAAC               GT[CT]^[GA]AC
HindII          3  GTCAAC               GT[CT]^[GA]AC
Ksp632I       144  GAAGAG               CTCTTC(1/4)
MfeI          116  CAATTG               C^AATTG
MunI          116  CAATTG               C^AATTG
NlaIV          78  GGCACC               GG[ACGT]^[ACGT]CC
NspI          185  ACATGC               [GA]CATG^[CT]
NspV           62  TTCGAA               TT^CGAA
RsaI           88  GTAC                 GT^AC
SapI          144  GAAGAGC              GCTCTTC(1/4)
SfuI           62  TTCGAA               TT^CGAA
StyI          201  CCAAGG               C^C[AT][AT]GG
TaqI           63  TCGA                 T^CGA
TthHB8I        63  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AlwI          180  GATCC                GGATC(4/5)
               49  GGATC               
BbvI          112  GCAGC                GCAGC(8/12)
              196  GCAGC               
Fnu4HI        112  GCAGC                GC^[ACGT]GC
              196  GCAGC               
ItaI          112  GCAGC                GC^[ACGT]GC
              196  GCAGC               
MboII          40  GAAGA                GAAGA(8/7)
              144  GAAGA               
MnlI           37  GAGG                 CCTC(7/6)
               76  GAGG                
MslI          184  CACATGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              186  CATGCGTGTG          
NdeI           32  CATATG               CA^TATG
              125  CATATG              
NlaIII        186  CATG                 CATG^
              246  CATG                
TseI          112  GCAGC                G^C[AT]GC
              196  GCAGC               
Tsp509I       117  AATT                 ^AATT
              257  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

DpnII       MboI        MwoI        NdeII       Sau3AI