Restriction map for
pF1KB6016
##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccI AgeI AhdI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AviII AvrII BbvCI BlnI
BlpI Bpu1102I BsmBI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoO65I FseI FspI HgaI HindIII
HpaI MfeI MluI MunI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 872 GGTACC G^GTACC
AccIII 1993 TCCGGA T^CCGGA
AclI 548 AACGTT AA^CGTT
AcyI 90 GGCGCC G[GA]^CG[CT]C
AflII 2785 CTTAAG C^TTAAG
AflIII 2242 ACGTGT A^C[GA][CT]GT
Alw44I 2004 GTGCAC G^TGCAC
AocI 2452 CCTCAGG CC^T[ACGT]AGG
ApaLI 2004 GTGCAC G^TGCAC
Asp718I 872 GGTACC G^GTACC
BbeI 90 GGCGCC GGCGC^C
BbrPI 2241 CACGTG CAC^GTG
BciVI 2374 GGATAC GTATCC(6/5)
BclI 674 TGATCA T^GATCA
BfrI 2785 CTTAAG C^TTAAG
BglII 1173 AGATCT A^GATCT
BsaAI 2241 CACGTG [CT]AC^GT[GA]
BsaBI 1720 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 90 GGCGCC G[GA]^CG[CT]C
BsaI 2685 GAGACC GGTCTC(1/5)
BsaWI 1993 TCCGGA [AT]^CCGG[AT]
BseAI 1993 TCCGGA T^CCGGA
BseRI 1426 GAGGAG GAGGAG(10/8)
BsiEI 78 CGGCCG CG[GA][CT]^CG
BsiWI 134 CGTACG C^GTACG
BsmAI 2685 GAGAC GTCTC(1/5)
BspEI 1993 TCCGGA T^CCGGA
BsrBI 8 GAGCGG CCGCTC(-3/-3)
BstAPI 2267 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2452 CCTCAGG CC^T[ACGT]AGG
CfoI 91 GCGC GCG^C
DraI 1314 TTTAAA TTT^AAA
EagI 78 CGGCCG C^GGCCG
EclXI 78 CGGCCG C^GGCCG
Eco52I 78 CGGCCG C^GGCCG
Eco81I 2452 CCTCAGG CC^T[ACGT]AGG
EcoNI 1281 CCTAGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 674 TGATCA T^GATCA
HaeII 90 GGCGCC [GA]GCGC^[CT]
HhaI 91 GCGC GCG^C
Hin1I 90 GGCGCC G[GA]^CG[CT]C
HinP1I 91 GCGC G^CGC
HincII 223 GTTGAC GT[CT]^[GA]AC
HindII 223 GTTGAC GT[CT]^[GA]AC
KasI 90 GGCGCC G^GCGCC
KpnI 872 GGTACC GGTAC^C
MamI 1720 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1993 TCCGGA T^CCGGA
NaeI 125 GCCGGC GCC^GGC
NarI 90 GGCGCC GG^CGCC
NdeI 1203 CATATG CA^TATG
NgoMIV 125 GCCGGC G^CCGGC
PaeR7I 383 CTCGAG C^TCGAG
PmaCI 2241 CACGTG CAC^GTG
PmlI 2241 CACGTG CAC^GTG
Psp1406I 548 AACGTT AA^CGTT
PstI 2274 CTGCAG CTGCA^G
SfoI 90 GGCGCC GGC^GCC
SspI 803 AATATT AAT^ATT
XbaI 2140 TCTAGA T^CTAGA
XhoI 383 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 27 CGCG CG^CG
51 CGCG
AvaI 383 CTCGAG C^[CT]CG[GA]G
1999 CCCGAG
BalI 763 TGGCCA TGG^CCA
2802 TGGCCA
BamHI 1055 GGATCC G^GATCC
2301 GGATCC
BanII 1585 GAGCTC G[GA]GC[CT]^C
1647 GAGCTC
BbsI 1987 GAAGAC GAAGAC(2/6)
388 GTCTTC
BcnI 76 CCCGG CC^[GC]GG
81 CCGGG
BglI 4 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
46 GCCGCCGCGGC
BpuAI 1987 GAAGAC GAAGAC(2/6)
388 GTCTTC
BsoBI 383 CTCGAG C^[CT]CG[GA]G
1999 CCCGAG
BspHI 152 TCATGA T^CATGA
641 TCATGA
BsrFI 125 GCCGGC [GA]^CCGG[CT]
2688 ACCGGC
BstUI 27 CGCG CG^CG
51 CGCG
Cfr10I 125 GCCGGC [GA]^CCGG[CT]
2688 ACCGGC
DraII 719 AGGTCCC [GA]G^G[ACGT]CC[CT]
1601 AGGTCCT
EcoO109I 719 AGGTCCC [GA]G^G[ACGT]CC[CT]
1601 AGGTCCT
EcoRI 1460 GAATTC G^AATTC
2580 GAATTC
EcoRV 166 GATATC GAT^ATC
446 GATATC
EcoT14I 1627 CCATGG C^C[AT][AT]GG
2316 CCATGG
EcoT22I 421 ATGCAT ATGCA^T
2478 ATGCAT
FauI 105 CCCGC CCCGC(4/6)
28 GCGGG
KspI 26 CCGCGG CCGC^GG
50 CCGCGG
MluNI 763 TGGCCA TGG^CCA
2802 TGGCCA
MscI 763 TGGCCA TGG^CCA
2802 TGGCCA
MvnI 27 CGCG CG^CG
51 CGCG
NciI 76 CCCGG CC^[GC]GG
81 CCGGG
NcoI 1627 CCATGG C^CATGG
2316 CCATGG
NsiI 421 ATGCAT ATGCA^T
2478 ATGCAT
PflMI 1202 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1909 CCACTGAATGG
PpuMI 719 AGGTCCC [GA]G^G[AT]CC[CT]
1601 AGGTCCT
RcaI 152 TCATGA T^CATGA
641 TCATGA
SacI 1585 GAGCTC GAGCT^C
1647 GAGCTC
SacII 26 CCGCGG CCGC^GG
50 CCGCGG
SfcI 1046 CTACAG C^T[GA][CT]AG
2274 CTGCAG
SphI 1127 GCATGC GCATG^C
2210 GCATGC
StyI 1627 CCATGG C^C[AT][AT]GG
2316 CCATGG
Tsp45I 563 GTGAC ^GT[GC]AC
961 GTGAC
Van91I 1202 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1909 CCACTGAATGG
XcmI 1220 CCAAAAACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2607 CCAGATAAGTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BbvI BcgI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI