Restriction map for
pF1KF0137
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BlnI BsaBI
BsiEI BsiWI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Cfr10I CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoT22I FbaI FseI FspI KasI
KpnI MamI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SwaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 3695 TCCGGA T^CCGGA
AcyI 3848 GGCGTC G[GA]^CG[CT]C
Aor51HI 1290 AGCGCT AGC^GCT
ApaI 123 GGGCCC GGGCC^C
AseI 2458 ATTAAT AT^TAAT
AsnI 2458 ATTAAT AT^TAAT
AspI 3055 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2079 GGATCC G^GATCC
BbvCI 3078 CCTCAGC CCTCAGC(-5/-2)
BglII 2790 AGATCT A^GATCT
BsaAI 1828 TACGTG [CT]AC^GT[GA]
BsaHI 3848 GGCGTC G[GA]^CG[CT]C
BseAI 3695 TCCGGA T^CCGGA
BsmBI 951 GAGACG CGTCTC(1/5)
Bsp1407I 1611 TGTACA T^GTACA
BspDI 2268 ATCGAT AT^CGAT
BspEI 3695 TCCGGA T^CCGGA
BspMI 3585 ACCTGC ACCTGC(4/8)
BsrGI 1611 TGTACA T^GTACA
BstBI 752 TTCGAA TT^CGAA
ClaI 2268 ATCGAT AT^CGAT
DraIII 2965 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2039 GACATCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 13 CCGCGG C^C[GA][CT]GG
Eco47III 1290 AGCGCT AGC^GCT
EcoNI 2158 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3859 GAATTC G^AATTC
EcoRV 333 GATATC GAT^ATC
FauI 50 CCCGC CCCGC(4/6)
Hin1I 3848 GGCGTC G[GA]^CG[CT]C
HpaI 4299 GTTAAC GTT^AAC
KspI 13 CCGCGG CCGC^GG
MroI 3695 TCCGGA T^CCGGA
NspV 752 TTCGAA TT^CGAA
PaeR7I 356 CTCGAG C^TCGAG
PflFI 3055 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 99 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2458 ATTAAT AT^TAAT
SacII 13 CCGCGG CCGC^GG
SalI 2048 GTCGAC G^TCGAC
ScaI 4060 AGTACT AGT^ACT
SfuI 752 TTCGAA TT^CGAA
SphI 4089 GCATGC GCATG^C
SspBI 1611 TGTACA T^GTACA
SspI 1683 AATATT AAT^ATT
Tth111I 3055 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3293 CCAACAACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 356 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 2048 GTCGAC GT^[AC][GT]AC
3338 GTCTAC
Alw44I 1831 GTGCAC G^TGCAC
2119 GTGCAC
AocI 2209 CCTAAGG CC^T[ACGT]AGG
2429 CCTCAGG
ApaLI 1831 GTGCAC G^TGCAC
2119 GTGCAC
BglI 77 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4002 GCCAAACAGGC
BlpI 1322 GCTTAGC GC^T[ACGT]AGC
3636 GCTGAGC
Bpu1102I 1322 GCTTAGC GC^T[ACGT]AGC
3636 GCTGAGC
BsmI 684 GAATGC GAATGC(1/-1)
4052 GAATGC
BsrDI 927 GCAATG GCAATG(2/0)
1661 GCAATG
Bsu36I 2209 CCTAAGG CC^T[ACGT]AGG
2429 CCTCAGG
CelII 1322 GCTTAGC GC^T[ACGT]AGC
3636 GCTGAGC
Eco81I 2209 CCTAAGG CC^T[ACGT]AGG
2429 CCTCAGG
HgaI 18 GACGC GACGC(5/10)
3849 GCGTC
MaeII 1423 ACGT A^CGT
1829 ACGT
NspI 312 ACATGC [GA]CATG^[CT]
4089 GCATGC
SacI 4072 GAGCTC GAGCT^C
4462 GAGCTC
SapI 2014 GAAGAGC GCTCTTC(1/4)
3492 GAAGAGC
SmaI 70 CCCGGG CCC^GGG
126 CCCGGG
XbaI 769 TCTAGA T^CTAGA
2398 TCTAGA
XmaCI 70 CCCGGG C^CCGGG
126 CCCGGG
XmaI 70 CCCGGG C^CCGGG
126 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI